Beta Arrestin 1/2 antibody | 21-B1
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Mouse anti beta arrestin-11 antibody, clone 21-B1 recognises beta arrestin-1 and beta arrestin-2, members of the arrestin family, originally characterised as proteins involved in mediating the desensitization of G protein-coupled receptors (GPCR) by endocytosis. Beta arrestin 2 may also play a role in the down-regulation of TGF- beta receptor signalling. In addition, beta arrestins may also be involved in chemotaxis, cellular migration and metastasis.
In quiescent cells, beta arrestin is mainly located in the cytosol but translocates to the membrane as a result of GPCR receptor phosphorylation.
In quiescent cells, beta arrestin is mainly located in the cytosol but translocates to the membrane as a result of GPCR receptor phosphorylation.
Product Details
- Target Species
- Synthetic Peptide
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Hamster Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Carrier Free
- Yes
- Immunogen
- Beta arrestin-1 peptide.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
More Information
- UniProt
- P49407
- P32121
- Entrez Gene
- ARRB2
- ARRB1
- GO Terms
- GO:0005886 plasma membrane
- GO:0000139 Golgi membrane
- GO:0000785 chromatin
- GO:0002031 G-protein coupled receptor internalization
- GO:0005829 cytosol
- GO:0004857 enzyme inhibitor activity
- GO:0005096 GTPase activator activity
- GO:0005159 insulin-like growth factor receptor binding
- GO:0016567 protein ubiquitination
- GO:0005624 membrane fraction
- GO:0005625 soluble fraction
- GO:0005634 nucleus
- GO:0005765 lysosomal membrane
- GO:0005905 coated pit
- GO:0006366 transcription from RNA polymerase II promoter
- GO:0006892 post-Golgi vesicle-mediated transport
- GO:0007600 sensory perception
- GO:0008134 transcription factor binding
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010843 promoter binding
- GO:0015031 protein transport
- GO:0030168 platelet activation
- GO:0030659 cytoplasmic vesicle membrane
- GO:0031143 pseudopodium
- GO:0031397 negative regulation of protein ubiquitination
- GO:0031625 ubiquitin protein ligase binding
- GO:0031701 angiotensin receptor binding
- GO:0032088 negative regulation of NF-kappaB transcription factor activity
- GO:0032715 negative regulation of interleukin-6 production
- GO:0032717 negative regulation of interleukin-8 production
- GO:0035025 positive regulation of Rho protein signal transduction
- GO:0035066 positive regulation of histone acetylation
- GO:0043149 stress fiber assembly
- GO:0043161 proteasomal ubiquitin-dependent protein catabolic process
- GO:0043967 histone H4 acetylation
- GO:0070374 positive regulation of ERK1 and ERK2 cascade
- Regulatory
- For research purposes only
Applications of Beta Arrestin 1/2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoprecipitation | |||
| Western Blotting | 1/500 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Beta Arrestin 1/2 antibody
-
Hüttenrauch, F. et al. (2005) G protein-coupled receptor kinases promote phosphorylation and beta-arrestin-mediated internalization of CCR5 homo- and hetero-oligomers.
J Biol Chem. 280 (45): 37503-15. -
Ogasawara, F. et al. (2016) Concurrent Fowlpox and Candidiasis Diseases in Backyard Chickens with Unusual Pox Lesions in the Bursa of Fabricius.
Avian Dis. 60 (3): 705-8.
