Beta Arrestin 1/2 antibody | 21-B1

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Mouse anti Beta Arrestin 1/2

Product Type
Monoclonal Antibody
Clone
21-B1
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2557 IP WB 0.1 mg
Mouse anti beta arrestin-11 antibody, clone 21-B1 recognises beta arrestin-1 and beta arrestin-2, members of the arrestin family, originally characterised as proteins involved in mediating the desensitization of G protein-coupled receptors (GPCR) by endocytosis. Beta arrestin 2 may also play a role in the down-regulation of TGF- beta receptor signalling. In addition, beta arrestins may also be involved in chemotaxis, cellular migration and metastasis.

In quiescent cells, beta arrestin is mainly located in the cytosol but translocates to the membrane as a result of GPCR receptor phosphorylation.

Product Details

Target Species
Synthetic Peptide
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Hamster
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Beta arrestin-1 peptide.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P49407 Related reagents
P32121 Related reagents
Entrez Gene
ARRB2 Related reagents
ARRB1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0000139 Golgi membrane
GO:0000785 chromatin
GO:0002031 G-protein coupled receptor internalization
GO:0005829 cytosol
GO:0004857 enzyme inhibitor activity
GO:0005096 GTPase activator activity
GO:0005159 insulin-like growth factor receptor binding
GO:0016567 protein ubiquitination
GO:0005624 membrane fraction
GO:0005625 soluble fraction
GO:0005634 nucleus
GO:0005765 lysosomal membrane
GO:0005905 coated pit
GO:0006366 transcription from RNA polymerase II promoter
GO:0006892 post-Golgi vesicle-mediated transport
GO:0007600 sensory perception
GO:0008134 transcription factor binding
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010843 promoter binding
GO:0015031 protein transport
GO:0030168 platelet activation
GO:0030659 cytoplasmic vesicle membrane
GO:0031143 pseudopodium
GO:0031397 negative regulation of protein ubiquitination
GO:0031625 ubiquitin protein ligase binding
GO:0031701 angiotensin receptor binding
GO:0032088 negative regulation of NF-kappaB transcription factor activity
GO:0032715 negative regulation of interleukin-6 production
GO:0032717 negative regulation of interleukin-8 production
GO:0035025 positive regulation of Rho protein signal transduction
GO:0035066 positive regulation of histone acetylation
GO:0043149 stress fiber assembly
GO:0043161 proteasomal ubiquitin-dependent protein catabolic process
GO:0043967 histone H4 acetylation
GO:0070374 positive regulation of ERK1 and ERK2 cascade
Regulatory
For research purposes only

Applications of Beta Arrestin 1/2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for Beta Arrestin 1/2 antibody

  1. H├╝ttenrauch, F. et al. (2005) G protein-coupled receptor kinases promote phosphorylation and beta-arrestin-mediated internalization of CCR5 homo- and hetero-oligomers.
    J Biol Chem. 280 (45): 37503-15.
  2. Ogasawara, F. et al. (2016) Concurrent Fowlpox and Candidiasis Diseases in Backyard Chickens with Unusual Pox Lesions in the Bursa of Fabricius.
    Avian Dis. 60 (3): 705-8.