MHC Class I Monomorphic antibody | 41.17


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F FN ResetMouse anti Sheep MHC Class I Monomorphic
- Product Type
- Monoclonal Antibody
- Clone
- 41.17
- Isotype
- IgG1
- Specificity
- MHC Class I Monomorphic
Mouse anti Sheep MHC Class I antibody, clone 41.17 recognizes a monomorphic determinant on the class I MHC. The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In sheep, this complex is referred to as the ovine leukocyte antigen (OLA) region. Ovine MHC class I molecules are expressed at varying levels on the majority of nucleated cells. Mouse anti Sheep MHC Class I antibody, clone 41.17 immunoprecipitates a band of ~44 kDa under reducing conditions and has also been reported to react with paraffin-embedded material following alcohol fixation. |
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Ovine lymphocytes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3-NS/11-Ag4-1 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | ![]() |
1/50 | 1/100 |
Immunohistology - Frozen | ![]() |
||
Immunoprecipitation | ![]() |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1 x 106 cells in 100μl
Source Reference
-
Gogolin-Ewens, K.J. et al. (1985) Sheep lymphocyte antigens (OLA). I. Major histocompatibility complex class I molecules.
Immunology 56: 717-23.
References for MHC Class I Monomorphic antibody
-
Puri, N.K. et al. (1987) Monoclonal antibodies to sheep MHC class I and class II molecules: biochemical characterization of three class I gene products and four distinct subpopulations of class II molecules.
Vet Immunol Immunopathol. 15: 59-86. -
Wooldridge A.L. et al. (2019) Maternal allergic asthma during pregnancy alters fetal lung and immune development in sheep: potential mechanisms for programming asthma and allergy.
J Physiol. 597 (16): 4251-62. -
Berger, S.T. and Griffin, F.T. (2006) A comparison of ovine monocyte-derived macrophage function following infection with Mycobacterium avium ssp. avium and Mycobacterium avium ssp. paratuberculosis.
Immunol Cell Biol. 84 (4): 349-56. -
Foulon, E. and Foucras, G. (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression.
J Immunol Methods. 339 (1): 1-10. -
Godoy, R.F. et al. (2014) Do progenitor cells from different tissue have the same phenotype?
Res Vet Sci. 96 (3): 454-9. -
Rojas, J.M. et al. (2018) Peste des Petits Ruminants Virus Fusion and Hemagglutinin Proteins Trigger Antibody-Dependent Cell-Mediated Cytotoxicity in Infected Cells.
Front Immunol. 9: 3172.
MCA2224GA

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