MHC Class I Monomorphic antibody | 41.17

Mouse anti Sheep MHC Class I Monomorphic

Product Type
Monoclonal Antibody
Clone
41.17
Isotype
IgG1
Specificity
MHC Class I Monomorphic

Product Code Applications Pack Size List Price Your Price Qty
MCA2224GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Sheep MHC Class I antibody, clone 41.17 recognizes a monomorphic determinant on the class I MHC. The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In sheep, this complex is referred to as the ovine leukocyte antigen (OLA) region. Ovine MHC class I molecules are expressed at varying levels on the majority of nucleated cells.

Mouse anti Sheep MHC Class I antibody, clone 41.17 immunoprecipitates a band of ~44 kDa under reducing conditions and has also been reported to react with paraffin-embedded material following alcohol fixation.

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Ovine lymphocytes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3-NS/11-Ag4-1 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul

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Source Reference

  1. Gogolin-Ewens, K.J. et al. (1985) Sheep lymphocyte antigens (OLA). I. Major histocompatibility complex class I molecules.
    Immunology 56: 717-23.

References for MHC Class I Monomorphic antibody

  1. Puri, N.K. et al. (1987) Monoclonal antibodies to sheep MHC class I and class II molecules: biochemical characterization of three class I gene products and four distinct subpopulations of class II molecules.
    Vet Immunol Immunopathol. 15: 59-86.
  2. Wooldridge A.L. et al. (2019) Maternal allergic asthma during pregnancy alters fetal lung and immune development in sheep: potential mechanisms for programming asthma and allergy.
    J Physiol. 597 (16): 4251-62.
  3. Berger, S.T. and Griffin, F.T. (2006) A comparison of ovine monocyte-derived macrophage function following infection with Mycobacterium avium ssp. avium and Mycobacterium avium ssp. paratuberculosis.
    Immunol Cell Biol. 84 (4): 349-56.
  4. Foulon, E. and Foucras, G. (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression.
    J Immunol Methods. 339 (1): 1-10.
  5. Godoy, R.F. et al. (2014) Do progenitor cells from different tissue have the same phenotype?
    Res Vet Sci. 96 (3): 454-9.
  6. Rojas, J.M. et al. (2018) Peste des Petits Ruminants Virus Fusion and Hemagglutinin Proteins Trigger Antibody-Dependent Cell-Mediated Cytotoxicity in Infected Cells.
    Front Immunol. 9: 3172.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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