MHC Class I Monomorphic antibody | 41.17
Mouse anti Sheep MHC Class I antibody, clone 41.17 (MCA2224GA) used as a positive control for cell lysis experiments in vitro on pprv infected cells.
Image caption:
Sera from PPRV-immune sheep can mediate ADCC against PPRV infected cells. (A) Gating strategy for flow cytometry-based ADCC assays. FSC/SSC dot plots discriminated debris and allowed for event gating. Target cells (PKH67+ events) were then gated to perform the viability analysis with propidium iodide (PI) staining. Gating for dead cells was based on PI unstained samples. Maximum target cell death was measured by addition of 0.2% saponin. (B,C) Examples of cytometry dot-plots (duplicates from three independent experiments for each sera) used to measure specific lysis of target cells by effector cells. Representative dot plots of (B) BTV- or (C) PPRV-infected B cell enriched fraction (target cells) cultured in the absence of effector cells (spontaneous cell death) or in presence of effector cells (autologous NK/T cell fraction) after incubation with anti-MHC-I antibody as positive control, no serum, naïve serum, or PPRV-immune serum. (D) Mean (±SEM) specific lysis in three independent experiments of BTV-infected cells incubated with anti-MHC-I antibody as positive control, no serum, naïve, or BTV-immune serum at different effector to target cell ratio (E: T). ***p <0.001 Two-way ANOVA with Dunnett's post-test (anti-MHC-I vs. controls (no serum or naïve serum). (E) Mean (±SEM) specific lysis in three independent experiments against PPRV-infected cells incubated with anti-MHC-I antibody as positive control, no serum, PPRV-naïve serum A, B or C, or PPRV-immune serum A, B or C at different effector to target cell ratio (E: T). **p <0.01; ***p <0.001 Two-way ANOVA with Dunnett's post-test [anti-MHC-I and immune serum vs. controls (no serum and naïve serum)].
From: Rojas JM, Rodríguez-Martín D, Avia M, Martín V, Sevilla N.
Peste des Petits Ruminants Virus Fusion and Hemagglutinin Proteins Trigger Antibody-Dependent Cell-Mediated Cytotoxicity in Infected Cells.
Front Immunol. 2019 Jan 14;9:3172.
doi: 10.3389/fimmu.2018.03172.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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F FN ResetMouse anti Sheep MHC Class I Monomorphic
- Product Type
- Monoclonal Antibody
- Clone
- 41.17
- Isotype
- IgG1
- Specificity
- MHC Class I Monomorphic
Antibody Quality and Performance Guarantee
| Mouse anti Sheep MHC Class I antibody, clone 41.17 recognizes a monomorphic determinant on the class I MHC. The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In sheep, this complex is referred to as the ovine leukocyte antigen (OLA) region. Ovine MHC class I molecules are expressed at varying levels on the majority of nucleated cells. Mouse anti Sheep MHC Class I antibody, clone 41.17 immunoprecipitates a band of ~44 kDa under reducing conditions and has also been reported to react with paraffin-embedded material following alcohol fixation. |
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Ovine lymphocytes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3-NS/11-Ag4-1 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1 x 106 cells in 100μl
Source Reference
-
Gogolin-Ewens, K.J. et al. (1985) Sheep lymphocyte antigens (OLA). I. Major histocompatibility complex class I molecules.
Immunology 56: 717-23.
References for MHC Class I Monomorphic antibody
-
Puri, N.K. et al. (1987) Monoclonal antibodies to sheep MHC class I and class II molecules: biochemical characterization of three class I gene products and four distinct subpopulations of class II molecules.
Vet Immunol Immunopathol. 15: 59-86. -
Wooldridge A.L. et al. (2019) Maternal allergic asthma during pregnancy alters fetal lung and immune development in sheep: potential mechanisms for programming asthma and allergy.
J Physiol. 597 (16): 4251-62. -
Berger, S.T. and Griffin, F.T. (2006) A comparison of ovine monocyte-derived macrophage function following infection with Mycobacterium avium ssp. avium and Mycobacterium avium ssp. paratuberculosis.
Immunol Cell Biol. 84 (4): 349-56. -
Foulon, E. and Foucras, G. (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression.
J Immunol Methods. 339 (1): 1-10. -
Godoy, R.F. et al. (2014) Do progenitor cells from different tissue have the same phenotype?
Res Vet Sci. 96 (3): 454-9. -
Rojas, J.M. et al. (2018) Peste des Petits Ruminants Virus Fusion and Hemagglutinin Proteins Trigger Antibody-Dependent Cell-Mediated Cytotoxicity in Infected Cells.
Front Immunol. 9: 3172.
MCA2224GA
1601
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