GM-CSF antibody | 3C2
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Mouse anti Sheep GM-CSF
- Product Type
- Monoclonal Antibody
- Clone
- 3C2
- Isotype
- IgG1
- Specificity
- GM-CSF
Antibody Quality and Performance Guarantee
| Mouse anti Sheep GM-CSF, clone 3C2 recognizes both recombinant and native ovine Granulocyte-macrophage colony-stimulating factor, also known GM-CSF or CSF, a 16.3kDa cytokine responsible for the stimulation and growth of cells from granulocyte, macrophage, eosinophil and erythrocyte hematopoietic precursor cell linages. Mouse anti Sheep GM-CSF, clone 3C2 has is of particular interest as it has been shown to reconize GM-CSF in cattle while other clones such as 8D8 do not crosss react with cattle.. Mouse anti Sheep GM-CSF, clone 3C2 has been reported to neutralise the activity of both recombinant ovine and native GM-CSF. Removal of sodium azide is recommended prior to use in functional assays. |
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- CHO expressed recombinant ovine GM-CSF.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the NSO myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This reagent was evaluated at the Moredun Research Institute and The Roslin Institute at the University of Edinburgh as part of ‘The route to identification of immunological correlates of protection in ruminants’ Industrial Partnership Award funded by BBSRC (grant numbers BB/I019863/1; BB/I020519/1) with the support of Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- Mouse anti Sheep GM-CSF antibody, clone 3C2 (MCA1924 can be used in a sandwich ELISA as a capture antibody together with Mouse anti Sheep GM-CSF antibody, clone 8D8 (MCA1923) as the detection antibody. Conjugation of the detection antibody with LYNX Rapid or Rapid Plus Kits is recommended.
References for GM-CSF antibody
-
John, H.A. et al. (1994) Generation of an ovine bone marrow-derived myelomonocyte-like cell line by retroviral-mediated transformation. Immunological characterization and the effect of cytokines and lipopolysaccharides.
J Leukoc Biol. 55: 785-92. -
Haig, D.M. et al. (1995) Haemopoietic cell responses in the blood and bone marrow of sheep infected with the abomasal nematode Telodorsagia circumcincta.
J Comp Pathol. 112: 151-64. -
Entrican, G. et al. (1996) Development of a sandwich ELISA for ovine granulocyte/macrophage colony-stimulating factor.
Vet Immunol Immunopathol. 50 (1-2): 105-15. -
Deane, D. et al. (2000) Orf virus encodes a novel secreted protein inhibitor of granulocyte-macrophage colony-stimulating factor and interleukin-2.
J Virol. 74: 1313-20. -
Kennedy, H.E. et al. (2002) Modulation of immune responses to Mycobacterium bovis in cattle depleted of WC1(+) gamma delta T cells.
Infect Immun. 70: 1488-500. -
McInnes, C.J. et al. (2005) Glycosylation, disulfide bond formation, and the presence of a WSXWS-like motif in the orf virus GIF protein are critical for maintaining the integrity of Binding to ovine granulocyte-macrophage colony-stimulating factor and interleukin-2.
J Virol. 79: 11205-13. -
Scott, J.L. et al. (2007) Granulocyte-macrophage colony stimulating factor and interleukin-8 in the reproductive tract of ewes following oestrus and mating.
Reprod Fertil Dev. 19: 585-93. -
Foulon, E. and Foucras, G. (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression.
J Immunol Methods. 339: 1-10.
View The Latest Product References
-
Scott, J.L. (2009) Spermatozoa and seminal plasma induce a greater inflammatory response in the ovine uterus at oestrus than dioestrus.
Reprod Fertil Dev. 21: 817-26. -
Schnabel, C.L. et al. (2013) Evaluation of the reactivity of commercially available monoclonal antibodies with equine cytokines.
Vet Immunol Immunopathol. 156 (1-2): 1-19.
- Synonyms
- Granulocyte Macrophage Colony Stimulating Factor
- RRID
- AB_323204
- UniProt
- P28773
- Entrez Gene
- CSF2
- GO Terms
- GO:0005125 cytokine activity
- GO:0005129 granulocyte macrophage colony-stimulating factor receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
MCA1924
153284
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