CD230 (aa151 - aa159) antibody | 2G11
Mouse anti sheep CD230 (aa151 - aa159) antibody, clone 2G11 used for the detection of prion protein in ovine brain by Paraffin-embedded tissue blot analysis.
Image caption:
PET blot analysis identifying PrPSc in the tubular structures of the cortex and medulla in naturally scrapie-sick Sarda sheep. (A) Brains of scrapie-sick (left) and healthy sheep (right) as a control for PET blot analysis procedure. Scale bar = 100μm. (B) Low magnification of transverse paraffin sections from kidneys of a naturally scrapie-sick and a healthy sheep demonstrating PrPSc in the medullary and cortical regions of the kidney. Dotted lines indicate the locus of the kidney capsule or the edge of the tissue section. The small transparent box indicates the region shown at higher magnification and resolution in figure C. Scale bar = 1cm. (C) PrPSc deposits and tubular structure fit together in the cortical part of the kidney in scrapie-sick sheep. Scale bar = 100μm. (D) PrPSc is also found to a high degree in the collecting ducts of the medulla (arrowhead) as well as in the papillae (arrow) of kidneys derived from naturally scrapie infected sheep. Scale bar = 30μm.
From: Ligios C, Cancedda GM, Margalith I, Santucciu C, Madau L, et al. (2007)
Intraepithelial and Interstitial Deposition of Pathological Prion Protein in Kidneys of Scrapie-Affected Sheep.
PLoS ONE 2(9): e859.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Sheep CD230 (aa151 - aa159) antibody, clone 2G11 (MCA2460) used for the detection of prion protein in ovine brain by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemistry of brain in donor and recipient sheep. Trigeminal nucleus from donor (A) and recipient (B) animals; intraneuronal PrPSc is evident only in the recipient animal (B). White matter from donor (C) and recipient (D) animals; globular white matter labelling is evident only in the donor animal (C). Scale bar in (A) and (B) indicates 100μm; in (C) and (D) indicates 50 μm. Antibody 2G11
From: Simmons MM, Moore SJ, Lockey R, Chaplin MJ, Konold T, et al.. (2015) Phenotype Shift from Atypical Scrapie to CH1641 following Experimental Transmission in Sheep. PLoS ONE 10(2): e0117063.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti sheep CD230 (aa151 - aa159) antibody, clone 2G11 (MCA2460GA) used for the detection of prion protein in ovine brain by immunohistochemistry and immunofluorescence on formalin fixed, paraffin embedded tisues sections.
Image caption:
PET blot analysis identifying PrPSc in the tubular structures of the cortex and medulla in naturally scrapie-sick Sarda sheep. (A) Brains of scrapie-sick (left) and healthy sheep (right) as a control for PET blot analysis procedure. Scale bar = 100 µm. (B) Low magnification of transverse paraffin sections from kidneys of a naturally scrapie-sick and a healthy sheep demonstrating PrPSc in the medullary and cortical regions of the kidney. Dotted lines indicate the locus of the kidney capsule or the edge of the tissue section. The small transparent box indicates the region shown at higher magnification and resolution in figure C. Scale bar = 1 cm. (C) PrPSc deposits and tubular structure fit together in the cortical part of the kidney in scrapie-sick sheep. Scale bar = 100 µm. (D) PrPSc is also found to a high degree in the collecting ducts of the medulla (arrowhead) as well as in the papillae (arrow) of kidneys derived from naturally scrapie infected sheep. Scale bar = 30 µm.
From: Ligios C, Cancedda GM, Margalith I, Santucciu C, Madau L, et al. (2007)
Intraepithelial and Interstitial Deposition of Pathological Prion Protein in Kidneys of Scrapie-Affected Sheep.
PLoS ONE 2(9): e859.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Sheep CD230 (aa151 - aa159) antibody, clone 2G11 used for the detection of prion protein in scrapie infected sheep by immunohistohemistry.
Image caption:
Photomicrographs of retinal cross-sections of unaffected (a, d) and naturally scrapie-affected (b, c, e) sheep. All layers of the control retina show normal organization and cytoarchitecture (a). Ganglion cell layer (GCL), inner plexiform layer (IPL), inner nuclear layer (INL), outer plexiform layer (OPL), outer nuclear layer (ONL), photoreceptor segments (PS) and choroid (CHOR). Bar: 100 μm. In the scrapie-affected retina, not only was there a disorganization and thickening of the PS layer, but also an overall decrease of cellular density within the INL and ONL (b). Arrowhead indicates displaced photoreceptor nuclei in the PS layer. Bar: 100 μm. Immnohistochemical detection of abnormal PrP deposits (peroxydase-DAB, brown deposits-2G11 monoclonal mouse antibody) in a scrapie retina (c). Asterisks indicate the amorphous material accumulated in the photoreceptor outer segment layer. Bar: 100 μm. Müller cells labelling (GFAP rabbit polyclonal antibody peroxydase-DAB, brown coloration) in the retina from a control (d) and a naturally-scrapie affected sheep (e). Bars: 300 μm.
From: Regnier A, Andreoletti O, Albaric O, Gruson DC, Schelcher F, Toutain PL. Clinical, electroretinographic and histomorphometric evaluation of the retina in sheep with natural scrapie. BMC Vet Res. 2011 Jun 6;7:25.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Sheep CD230 (aa151 - aa159)
- Product Type
- Monoclonal Antibody
- Clone
- 2G11
- Isotype
- IgG2a
- Specificity
- CD230 (aa151 - aa159)
Antibody Quality and Performance Guarantee
| Mouse anti Sheep CD230 (aa151 - aa159) antibody, clone 2G11 recognizes CD230 and can be used to detect the plaques which are formed during the progression of prion disease. Transmissible spongiform encephalopathies (TSEs) or prion diseases are fatal infectious neurodegenerative diseases of humans and animals. These diseases are biologically unique, as they are believed by some to be transmitted by an infectious agent comprised only of protein, with no nucleic acid component. Clinically, these diseases present with motor disturbances and behavioural changes. The major pathological changes seen are neuronal loss, vacuolation (spongiform change), proliferation and branching of glial cells, astrocytic proliferation and accumulation of the prion protein PrPSc, which can form amyloid plaques. CD230. also known as the prion protein (PrP) exists in two alternate forms; a normal cellular form (PrPc) and a disease-associated form (PrPSc).The normal and pathological forms of the prion protein have identical amino acid sequences and differ only in their folded tertiary structure and biochemical properties. MCA2460 was raised against a synthetic peptide (146-R154R171-182 ) of the ovine PrP peptide and specifically recognises the R151-R159 sequence. |
- Target Species
- Sheep
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Synthetic peptide 146-R154R171-182 of ovine PrP.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/50 | 1/1000 |
| Immunohistology - Paraffin 1 | |
- 1Treatment of tissue sections in 98% formic acid, for 30 minutes, is recommended prior to pre-treatment with trypsin at 37oC for 5 minutes followed by heat mediated retrieval with 10mM citrate buffer pH6.0 See Andreoletti 2000.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- ELISA
- Tested on peptide.
References for CD230 (aa151 - aa159) antibody
-
Andréoletti, O. et al. (2000) Early accumulation of PrP(Sc) in gut-associated lymphoid and nervous tissues of susceptible sheep from a Romanov flock with natural scrapie.
J Gen Virol. 81 (Pt 12): 3115-26. -
Andréoletti, O. et al. (2002) Phenotyping of protein-prion (PrPsc)-accumulating cells in lymphoid and neural tissues of naturally scrapie-affected sheep by double-labeling immunohistochemistry.
J Histochem Cytochem. 50 (10): 1357-70. -
Borner, R. et al. (2011) Early behavioral changes and quantitative analysis of neuropathological features in murine prion disease: stereological analysis in the albino Swiss mice model.
Prion. 5 (3): 215-27. -
Ortiz-Pelaez, A. et al. (2015) Allelic variants at codon 146 in the PRNP gene show significant differences in the risk for natural scrapie in Cypriot goats.
Epidemiol Infect. 143 (6): 1304-10. -
Simmons, M.M. et al. (2015) Phenotype shift from atypical scrapie to CH1641 following experimental transmission in sheep.
PLoS One. 10 (2): e0117063.
- Synonyms
- Prion Protein
- RRID
- AB_808711
- UniProt
- P23907
- Entrez Gene
- PRNP
- GO Terms
- GO:0005886 plasma membrane
- GO:0005507 copper ion binding
- GO:0005794 Golgi apparatus
- GO:0008017 microtubule binding
- GO:0031225 anchored to membrane
- GO:0051260 protein homooligomerization
MCA2460
152956
164444
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