TCR V Beta 10 antibody | G101

Mouse anti Rat TCR V Beta 10

Product Type
Monoclonal Antibody
Clone
G101
Isotype
IgG2a
Specificity
TCR V Beta 10

Product Code Applications Pack Size List Price Your Price Qty
MCA937
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.25 mg loader
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loader
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Mouse anti Rat TCR V Beta 10 antibody, clone G101 recognizes the T-cell receptor V beta 10 gene product.

Approximately 4.2% of unseparated LEW lymph node cells were detected by this clone. Reactivity is restricted to alpha/beta T cells, of which 6.7% labeled (Torres-Nagel et al. 1993).

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
T cell clone expressing the TCR V beta 10 gene product.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells form immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Goat anti Mouse IgG/A/M:Alk. Phos.
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Mouse IgG2a Negative Control

References for TCR V Beta 10 antibody

  1. Torres-Nagel, N.E. et al. (1993) Identification of rat Tcrb-V8.2, 8.5, and 10 gene products by monoclonal antibodies.
    Immunogenetics. 37 (4): 305-8.
  2. Hanaoka, M. et al. (2010) Immunomodulatory strategies prevent the development of autoimmune emphysema.
    Respir Res. 11: 179.
  3. Park, J.H. et al. (1996) Identification and cellular distribution of the rat interleukin-2 receptor beta chain: induction of the IL-2R alpha- beta+ phenotype by major histocompatibility complex class I recognition during T cell development in vivo and by T cell receptor stimulation of CD4+8+ immature thymocytes in vitro.
    Eur J Immunol. 26: 2371-5
  4. Matsumoto, Y. et al. (2000) Successful TCR-based immunotherapy for autoimmune myocarditis with DNA vaccines after rapid identification of pathogenic TCR.
    J Immunol. 164 (4): 2248-54.

RRID
AB_322469
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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