TCR V Beta 10 antibody | G101

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Mouse anti Rat TCR V Beta 10

Product Type
Monoclonal Antibody
Clone
G101
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA937 F 0.25 mg
Mouse anti Rat TCR V Beta 10 antibody, clone G101 recognizes the T-cell receptor V beta 10 gene product.

Approximately 4.2% of unseparated LEW lymph node cells were detected by this clone. Reactivity is restricted to alpha/beta T cells, of which 6.7% labeled (Torres-Nagel et al. 1993).

Product Details

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
T cell clone expressing the TCR V beta 10 gene product.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells form immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of TCR V Beta 10 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2a Negative Control MCA1210 100 Tests F

Product Specific References

References for TCR V Beta 10 antibody

  1. Torres-Nagel, N.E. et al. (1993) Identification of rat Tcrb-V8.2, 8.5, and 10 gene products by monoclonal antibodies.
    Immunogenetics. 37 (4): 305-8.
  2. Hanaoka, M. et al. (2010) Immunomodulatory strategies prevent the development of autoimmune emphysema.
    Respir Res. 11: 179.
  3. Park, J.H. et al. (1996) Identification and cellular distribution of the rat interleukin-2 receptor beta chain: induction of the IL-2R alpha- beta+ phenotype by major histocompatibility complex class I recognition during T cell development in vivo and by T cell receptor stimulation of CD4+8+ immature thymocytes in vitro.
    Eur J Immunol. 26: 2371-5
  4. Matsumoto, Y. et al. (2000) Successful TCR-based immunotherapy for autoimmune myocarditis with DNA vaccines after rapid identification of pathogenic TCR.
    J Immunol. 164 (4): 2248-54.