Reticulum Cells antibody | ED11
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Mouse anti Rat reticulum cells antibody, clone ED11 recognizes reticular elements in the T cell areas and B cell follicles of spleen, lymph nodes, Peyer's patches, bronchus and gut associated lymphoid tissues (BALT and GALT). In the thymus a reticular pattern is observed throughout the cortex capsule, the medulla is negative. In non-lymphoid organs positivity is seen only in reticular elements of the small intestine. No staining of liver, lung, skin, kidney, brain, pancreas or heart is seen.
Biochemical analysis has revealed that Mouse anti Rat reticulum cells antibody, clone ED11 recognizes complement factor C3. In vivo studies have shown that Mouse anti Rat reticulum cells antibody, clone ED11 blocks immune complex trapping on follicular dendritic cells (Van den Berg et al. 1992).
Biochemical analysis has revealed that Mouse anti Rat reticulum cells antibody, clone ED11 recognizes complement factor C3. In vivo studies have shown that Mouse anti Rat reticulum cells antibody, clone ED11 blocks immune complex trapping on follicular dendritic cells (Van den Berg et al. 1992).
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Rat spleen cell homogenate.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with the mouse SP2/0.Ag14 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Reticulum Cells antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Flow Cytometry | |||
| Immunohistology - Frozen 1 | 1/100 | 1/300 | |
| Immunohistology - Paraffin |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
|
Product Specific References
Source Reference
-
Van den Berg, T.K. et al. (1989) The heterogeneity of the reticulum of rat peripheral lymphoid organs identified by monoclonal antibodies.
Eur J Immunol. 19 (9): 1747-56.
References for Reticulum Cells antibody
-
Van den Berg, T.K. et al. (1992) Selective inhibition of immune complex trapping by follicular dendritic cells with monoclonal antibodies against rat C3.
Eur J Immunol. 22 (4): 957-62. -
Sato K et al. (2001) Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat cardiac transplants.
J Immunol. 166 (6): 4185-94. -
Lin, Y. et al. (1999) Rejection of cardiac xenografts by CD4+ or CD8+ T cells.
J Immunol. 162 (2): 1206-14. -
Sato, K. et al. (1999) Suppression of delayed xenograft rejection by specific depletion of elicited antibodies of the IgM isotype.
Transplantation. 68 (6): 844-54. -
Molleví, D.G. et al. (2001) Heart and liver xenotransplantation under low-dose tacrolimus: graft survival after withdrawal of immunosuppression.
Transplantation. 71 (2): 217-23.
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