Reticulum Cells antibody | ED11

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Mouse anti Rat Reticulum Cells

Product Type: Monoclonal Antibody
Clone: ED11
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA733R	C* E F			0.25 mg

Mouse anti Rat reticulum cells antibody, clone ED11 recognizes reticular elements in the T cell areas and B cell follicles of spleen, lymph nodes, Peyers patches, bronchus and gut associated lymphoid tissues (BALT and GALT). In the thymus a reticular pattern is observed throughout the cortex capsule, the medulla is negative. In non-lymphoid organs positivity is seen only in reticular elements of the small intestine. No staining of liver, lung, skin, kidney, brain, pancreas or heart is seen.

Biochemical analysis has revealed that Mouse anti Rat reticulum cells antibody, clone ED11 recognises complement factor C3. In vivo studies have shown that Mouse anti Rat reticulum cells antibody, clone ED11 blocks immune complex trapping on follicular dendritic cells (Van den Berg et al. 1992).

Product Details

Target Species

Rat

Product Form

Purified IgG - liquid

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Carrier Free

Yes

Immunogen

Rat spleen cell homogenate.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunized BALB/c mice were fused with the mouse SP2/0.Ag14 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

Regulatory: For research purposes only

Applications of Reticulum Cells antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen ¹	1/100	1/300
Immunohistology - Paraffin

¹The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control	MCA1209	0.1 mg	F

Product Specific References

Source Reference

Van den Berg, T.K. et al. (1989) The heterogeneity of the reticulum of rat peripheral lymphoid organs identified by monoclonal antibodies.
Eur J Immunol. 19 (9): 1747-56.

References for Reticulum Cells antibody

Van den Berg, T.K. et al. (1992) Selective inhibition of immune complex trapping by follicular dendritic cells with monoclonal antibodies against rat C3.
Eur J Immunol. 22 (4): 957-62.
Sato K et al. (2001) Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat cardiac transplants.
J Immunol. 166 (6): 4185-94.
Lin, Y. et al. (1999) Rejection of cardiac xenografts by CD4+ or CD8+ T cells.
J Immunol. 162 (2): 1206-14.
Sato, K. et al. (1999) Suppression of delayed xenograft rejection by specific depletion of elicited antibodies of the IgM isotype.
Transplantation. 68 (6): 844-54.
Molleví, D.G. et al. (2001) Heart and liver xenotransplantation under low-dose tacrolimus: graft survival after withdrawal of immunosuppression.
Transplantation. 71 (2): 217-23.

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