Mouse anti Rat reticulum cells antibody, clone ED10 recognizes reticular elements in the T cell areas of spleen, lymph nodes, Peyers patches, bronchus and gut associated lymphoid tissues (BALT and GALT). In the thymus a reticular pattern is observed in capsule and medulla (perivascular). Non-lymphoid organs: in liver, lung and small intestine, some ED10 positive reticular cells are found. No reaction is observed in skin, kidney, brain, pancreas and heart.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Rat spleen cell homogenate.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 Ag14 myeloma cell line.
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Reticulum Cells antibody
|Immunohistology - Frozen 1
|Immunohistology - Paraffin
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Product Specific References
References for Reticulum Cells antibody
Van den Berg, T.K. et al. (1989) The heterogeneity of the reticulum of rat peripheral lymphoid organs identified by monoclonal antibodies.
Eur J Immunol. 19 (9): 1747-56.
Armstrong, R.J. et al. (2001) Porcine neural xenografts in the immunocompetent rat: immune response following grafting of expanded neural precursor cells.
Neuroscience. 106 (1): 201-16.
Soesatyo, M. (1992) Ontogeny of reticulum cells in the rat intestine and their possible role in the development of the lymphoid microenvironment.
Reg Immunol. 14: 46-52.
van Poppel, M.N. et al. (1993) Reticulum cells in the ontogeny of nasal-associated lymphoid tissue (NALT) in the rat.
Cell Tissue Res. 273: 577-81.