Reticulum Cells antibody | ED10
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Mouse anti Rat reticulum cells antibody, clone ED10 recognizes reticular elements in the T cell areas of spleen, lymph nodes, Peyers patches, bronchus and gut associated lymphoid tissues (BALT and GALT). In the thymus a reticular pattern is observed in capsule and medulla (perivascular). Non-lymphoid organs: in liver, lung and small intestine, some ED10 positive reticular cells are found. No reaction is observed in skin, kidney, brain, pancreas and heart.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Rat spleen cell homogenate.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 Ag14 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of Reticulum Cells antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen 1 | |||
| Immunohistology - Paraffin |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Reticulum Cells antibody
-
Van den Berg, T.K. et al. (1989) The heterogeneity of the reticulum of rat peripheral lymphoid organs identified by monoclonal antibodies.
Eur J Immunol. 19 (9): 1747-56. -
Armstrong, R.J. et al. (2001) Porcine neural xenografts in the immunocompetent rat: immune response following grafting of expanded neural precursor cells.
Neuroscience. 106 (1): 201-16. -
Soesatyo, M.
(1992) Ontogeny of reticulum cells in the rat intestine and their possible role in the development of the lymphoid microenvironment.
Reg Immunol. 14: 46-52. -
van Poppel, M.N. et al. (1993) Reticulum cells in the ontogeny of nasal-associated lymphoid tissue (NALT) in the rat.
Cell Tissue Res. 273: 577-81.
