Reticulum Cells antibody | ED10

Mouse anti Rat Reticulum Cells

Product Type
Monoclonal Antibody
Clone
ED10
Isotype
IgG1
Specificity
Reticulum Cells

Product Code Applications Pack Size List Price Your Price Qty
MCA732R
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * 0.25 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Rat reticulum cells antibody, clone ED10 recognizes reticular elements in the T cell areas of spleen, lymph nodes, Peyers patches, bronchus and gut associated lymphoid tissues (BALT and GALT). In the thymus a reticular pattern is observed in capsule and medulla (perivascular). Non-lymphoid organs: in liver, lung and small intestine, some ED10 positive reticular cells are found. No reaction is observed in skin, kidney, brain, pancreas and heart.

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Rat spleen cell homogenate.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 Ag14 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1
Immunohistology - Paraffin
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

References for Reticulum Cells antibody

  1. Van den Berg, T.K. et al. (1989) The heterogeneity of the reticulum of rat peripheral lymphoid organs identified by monoclonal antibodies.
    Eur J Immunol. 19 (9): 1747-56.
  2. Armstrong, R.J. et al. (2001) Porcine neural xenografts in the immunocompetent rat: immune response following grafting of expanded neural precursor cells.
    Neuroscience. 106 (1): 201-16.
  3. Soesatyo, M. (1992) Ontogeny of reticulum cells in the rat intestine and their possible role in the development of the lymphoid microenvironment.
    Reg Immunol. 14: 46-52.
  4. van Poppel, M.N. et al. (1993) Reticulum cells in the ontogeny of nasal-associated lymphoid tissue (NALT) in the rat.
    Cell Tissue Res. 273: 577-81.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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