OX-62 antibody | OX-62

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MCA1029G
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SDS Safety Datasheet SDS
C F IP WB 0.25 mg loader
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loader
MCA1029GA
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SDS Safety Datasheet SDS
C F IP 0.1 mg loader
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loader
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Mouse anti Rat OX-62 antibody, clone OX-62 recognizes the OX-62 antigen, also known as rat alpha E integrin or CD103. OX-62 appears as an 1150 amino acid, ~130 kDa single pass type I transmembrane protein expressed by intestinal dendritic cells, dendritic epidermal T cells, intraepithelial lymphocytes in the small intestine and by cells of dendritic morphology in lymphoid organs, at sites where gamma delta T cells are present.

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Density gradient enriched PVG rat thoracic duct dendritic cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells of BALB/c mice were fused with cells of the mouse NS0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen 1/25 1/100
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
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Description Mouse IgG1 Negative Control

References for OX-62 antibody

  1. Brenan, M. & Puklavec, M. (1992) The MRC OX-62 antigen: a useful marker in the purification of rat veiled cells with the biochemical properties of an integrin.
    J Exp Med. 175 (6): 1457-65.
  2. Brenan, M. & Rees, D.J. (1997) Sequence analysis of rat integrin alpha E1 and alpha E2 subunits: tissue expression reveals phenotypic similarities between intraepithelial lymphocytes and dendritic cells in lymph.
    Eur J Immunol. 27 (11): 3070-9.
  3. Kostulas, N. et al. (2002) Dendritic cells are present in ischemic brain after permanent middle cerebral artery occlusion in the rat.
    Stroke. 33 (4): 1129-34.
  4. Rival, C. et al. (2006) Identification of a dendritic cell population in normal testis and in chronically inflamed testis of rats with autoimmune orchitis.
    Cell Tissue Res. 324 (2): 311-8.
  5. Tsuchiya, T. et al. (2002) Dendritic cell involvement in pulmonary granuloma formation elicited by bacillus calmette-guérin in rats.
    Am J Respir Crit Care Med. 165 (12): 1640-6.
  6. Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and leukocyte infiltration in the transgenic rat model of tauopathy.
    J Neuroimmunol. 209 (1-2): 16-25.
  7. Schwartzkopff, J. et al. (2010) NK cell depletion delays corneal allograft rejection in baby rats.
    Mol Vis. 16: 1928-35.
  8. Baca Jones, C.C. et al. (2009) Rat cytomegalovirus infection depletes MHC II in bone marrow derived dendritic cells.
    Virology. 388: 78-90.
  9. View The Latest Product References
  10. Zhou, Y.J. et al. (2010) The role of the lactadherin in promoting intestinal DCs development in vivo and vitro.
    Clin Dev Immunol. 2010: 357541.
  11. Aiello, S. et al. (2000) Thymic Dendritic Cells Express Inducible Nitric Oxide Synthase and Generate Nitric Oxide in Response to Self- and Alloantigens
    J Immunol. 164: 4649-58.
  12. Camelo, S. et al. (2003) Local retention of soluble antigen by potential antigen-presenting cells in the anterior segment of the eye.
    Invest Ophthalmol Vis Sci. 44: 5212-9.
  13. Camelo, S. et al. (2004) The distribution of antigen in lymphoid tissues following its injection into the anterior chamber of the rat eye.
    J Immunol. 172: 5388-95.
  14. Henry, F. et al. (1999) Antigen-presenting cells that phagocytose apoptotic tumor-derived cells are potent tumor vaccines.
    Cancer Res. 59: 3329-32.
  15. Liu, L. et al. (1998) Dendritic cell heterogeneity in vivo: two functionally different dendritic cell populations in rat intestinal lymph can be distinguished by CD4 expression.
    J Immunol. 161: 1146-55.
  16. O'Sullivan, N.L. et al. (2001) Lymphocyte lineages at mucosal effector sites: rat salivary glands.
    J Immunol. 166: 5522-9.
  17. Penttila, I.A. et al. (2003) Maternal milk regulation of cell infiltration and interleukin 18 in the intestine of suckling rat pups.
    Gut. 52: 1579-86.
  18. Stephens, L.A. et al. (2004) Phenotypic characterization of regulatory CD4+CD25+ T cells in rats.
    Int Immunol. 16: 365-75.
  19. Sugawara, I. et al. (2004) Pathological and immunological profiles of rat tuberculosis.
    Int J Exp Pathol. 85: 125-34.
  20. Tiurbe, G. et al. (2009) Inhibitory effects of rat bone marrow-derived dendritic cells on naïve and alloantigen-specific CD4+ T cells: a comparison between dendritic cells generated with GM-CSF plus IL-4 and dendritic cells generated with GM-CSF plus IL-10.
    BMC Res Notes. 2: 12.
  21. Chen-Woan, M. et al. (1995) A new protocol for the propagation of dendritic cells from rat bone marrow using recombinant GM-CSF, and their quantification using the mAb OX-62.
    J Immunol Methods. 178 (2): 157-71.
  22. Stojić-Vukanić, Z. et al. (2016) Estradiol enhances capacity of TLR-matured splenic dendritic cells to polarize CD4+ lymphocytes into IL-17/GM-CSF-producing cells in vitro.
    Int Immunopharmacol. 40: 244-253.
  23. Varas, A. et al. (1998) Interleukin-7 influences the development of thymic dendritic cells.
    Blood. 92: 93-100.
  24. Bufan, B. et al. (2015) Aging Impairs Endocytic Capacity Of Splenic Dendritic Cells From Dark Agouti Rats And Alters Their Response To TLR4 Stimulation
    Acta Veterinaria. Volume 65, Issue 1, Pages 30–55.
  25. Syrjälä, S. et al. (2015) Donor Heart Treatment With COMP-Ang1 Limits Ischemia-Reperfusion Injury and Rejection of Cardiac Allografts.
    Am J Transplant. 15 (8): 2075-84.
  26. Palin, N.K. et al. (2017) Activin inhibition limits early innate immune response in rat kidney allografts-a pilot study.
    Transpl Int. 30 (1): 96-107.
  27. Raissadati, A. et al. (2017) Vascular Endothelial Growth Factor-B Overexpressing Hearts Are Not Protected From Transplant-Associated Ischemia-Reperfusion Injury.
    Exp Clin Transplant. 15 (2): 203-12.
  28. Stojić-Vukanić, Z. et al. (2019) Age and sex determine CD4+ T cell stimulatory and polarizing capacity of rat splenic dendritic cells.
    Biogerontology. Oct 23 [Epub ahead of print].
  29. Lauzon-Joset, J.F. et al. (2020) Oestrogen amplifies pre-existing atopy-associated Th2 bias in an experimental asthma model.
    Clin Exp Allergy. 50 (3): 391-400.
  30. Pilipović, I. et al. (2019) Noradrenaline modulates CD4+ T cell priming in rat experimental autoimmune encephalomyelitis: a role for the α1-adrenoceptor.
    Immunol Res. 67 (2-3): 223-40.
  31. Yu, J. et al. (2022) Topical Administration of 0.3% Tofacitinib Suppresses M1 Macrophage Polarization and Allograft Corneal Rejection by Blocking STAT1 Activation in the Rat Cornea
    Transl Vis Sci Technol. 11 (3): 34
  32. Cąkała-Jakimowicz, M. & Puzianowska-Kuznicka, M. (2022) Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis..
    Biomedicines. 10 (5): 1021.

Flow Cytometry

Immunocytochemistry

Immunohistology - Frozen

Synonyms
CD103
RRID
AB_2128733
UniProt
O88341
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