Neurofilament M antibody | 3H11

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Mouse anti Rat Neurofilament M (C-Terminal)

Product Type
Monoclonal Antibody
Clone
3H11
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA4752 IF WB 0.1 ml
Mouse anti Rat Neurofilament M antibody, clone 3H11 recognizes the carboxy terminus of rat neurofilament M (NF-M), one of three neurofilament proteins (NF-H, NF-M and NF-L). Neurofilaments are 10nm intermediate filaments found in neurons, with especially high concentrations along the axons, where they appear to control axonal diameter. It is thought that phosphorylation of NF-M results in the formation of interfilament cross-bridges that are important in the maintenance of axonal calibre.

Mouse anti Rat Neurofilament M antibody, clone 3H11 also binds to a C- terminal epitope of bovine NF-M.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Mouse
Chicken
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.065% Sodium Azide (NaN3)
Immunogen
Recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E.coli.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P12839 Related reagents
P08553 Related reagents
P07197 Related reagents
P16053 Related reagents
Entrez Gene
NEFM Related reagents
Nefm Related reagents
Nefm Related reagents
NEFM Related reagents
GO Terms
GO:0005515 protein binding
GO:0005200 structural constituent of cytoskeleton
GO:0005883 neurofilament
GO:0000226 microtubule cytoskeleton organization
GO:0005198 structural molecule activity
GO:0008088 axon cargo transport
GO:0031133 regulation of axon diameter
GO:0031594 neuromuscular junction
GO:0045110 intermediate filament bundle assembly
GO:0060052 neurofilament cytoskeleton organization
Regulatory
For research purposes only

Applications of Neurofilament M antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/100
Western Blotting 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA4572 detects a band of approximately 145kDa in rat cortex lysate.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Western Blotting

Product Specific References

References for Neurofilament M antibody

  1. Shaw, G. et al. (2002) Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells.
    FASEB J. 16: 869-71.
  2. Shaw, G. et al. (2004) Characterization of the bovine neurofilament NF-M protein and cDNA sequence, and identification of in vitro and in vivo calpain cleavage sites.
    Biochem Biophys Res Commun. 325: 619-25.

Further Reading

  1. Shaw, G. et al. (1997) Characterization of additional casein kinase I sites in the C-terminal "tail" region of chicken and rat neurofilament-M.
    J Neurochem. 69: 1729-37.