MHC Class II RT1D antibody | OX-17
Mouse anti Rat MHC Class II RT1D antibody, clone OX-17 (MCA50R) used for the identification of MHC class II expressing cells in vitro by immunofluorescence.
Image caption:
Immunofluorescence staining for MHC class II. a, non-stimulated conditions; b, after IFN-γ stimulation, c, IFN-γ + 10-8 M simvastatin; d, IFN-γ + 10-8 M simvastatin + 100 μM mevalonate
From: Zeinstra E, Wilczak N, Chesik D, Glazenburg L, Kroese FG, De Keyser J.
Simvastatin inhibits interferon-gamma-induced MHC class II up-regulation in cultured astrocytes.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
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IF ResetMouse anti Rat MHC Class II RT1D
- Product Type
- Monoclonal Antibody
- Clone
- OX-17
- Isotype
- IgG1
- Specificity
- MHC Class II RT1D
Quick Links:
Antibody Quality and 
Unrivaled 
| Mouse anti Rat MHC Class II RT1D antibody, clone OX-17 recognizes a monomorphic determinant on rat RT1D, the rat homologue of mouse I-E, present on all rat strains. Mouse anti Rat MHC Class II RT1D antibody, clone OX-17 does not cross-react with rat RT1B or mouse I-E antigen (Fukumoto et al. 1982). Mouse anti Rat MHC Class II RT1D antibody, clone OX-17 is routinely tested in flow cytometry on rat splenocytes. |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Rat spleen glycoproteins.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused with cells from the mouse X63.Ag8.653 myeloma cell line .
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/100 | |
| Immunohistology - Frozen | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for MHC Class II RT1D antibody
-
Fukumoto, T. et al. (1982) Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus.
Eur J Immunol. 12 (3): 237-43. -
Romaniuk, A. et al. (1995) Rejection of cartilage formed by transplanted allogeneic chondrocytes: evaluation with monoclonal antibodies.
Transpl Immunol. 3 (3): 251-7. -
Volovitz, I. et al. (2011) Split immunity: immune inhibition of rat gliomas by subcutaneous exposure to unmodified live tumor cells.
J Immunol. 187: 5452-62. -
Zeinstra, E. et al. (2006) Simvastatin inhibits interferon-gamma-induced MHC class II up-regulation in cultured astrocytes.
J Neuroinflammation. 3:16. -
Milićević, N.M. et al. (2005) T cells are required for the peripheral phase of B-cell maturation.
Immunology. 116: 308-17. -
Wildner, G. and Diedrichs-Möhring, M. (2003) Autoimmune uveitis induced by molecular mimicry of peptides from rotavirus, bovine casein and retinal S-antigen.
Eur J Immunol. 33: 2577-87. -
Sawa, K. and Mochizuki, M. (1997) Effects of bucillamine and antigen-presenting cells in experimental autoimmune uveitis in rats.
Jpn J Ophthalmol. 41: 388-95. -
Gurbuxani, S. et al. (2001) Selective depletion of inducible HSP70 enhances immunogenicity of rat colon cancer cells.
Oncogene. 20: 7478-85.
View The Latest Product References
-
Volovitz, I. et al. (2010) T cell vaccination induces the elimination of EAE effector T cells: analysis using GFP-transduced, encephalitogenic T cells.
J Autoimmun. 35: 135-44. -
Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and leukocyte infiltration in the transgenic rat model of tauopathy.
J Neuroimmunol. 209: 16-25. -
Ghiringhelli, F. et al. (2005) Tumor cells convert immature myeloid dendritic cells into TGF-beta-secreting cells inducing CD4+CD25+ regulatory T cell proliferatio
J Exp Med. 202: 919-29.
- RRID
- AB_323208
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