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MHC Class II RT1D antibody | OX-17

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Anti Rat MHC Class II RT1D Antibody, clone OX-17 gallery image 1
Published customer image:
Mouse anti Rat MHC Class II RT1D antibody, clone OX-17 (MCA50R) used for the identification of MHC class II expressing cells in vitro by immunofluorescence.
Image caption:
Immunofluorescence staining for MHC class II. a, non-stimulated conditions; b, after IFN-γ stimulation, c, IFN-γ + 10-8 M simvastatin; d, IFN-γ + 10-8 M simvastatin + 100 μM mevalonate

From: Zeinstra E, Wilczak N, Chesik D, Glazenburg L, Kroese FG, De Keyser J.
Simvastatin inhibits interferon-gamma-induced MHC class II up-regulation in cultured astrocytes.
J Neuroinflammation. 2006 Jul 21;3:16..
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Rat MHC Class II RT1D

Product Type
Monoclonal Antibody
Clone
OX-17
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA50GA
Datasheet
C F
SDS
0.1 mg loader

MCA50R
Datasheet
C F
SDS
0.25 mg loader

Mouse anti Rat MHC Class II RT1D antibody, clone OX-17 recognizes a monomorphic determinant on rat RT1D, the rat homologue of mouse I-E, present on all rat strains.

Mouse anti Rat MHC Class II RT1D antibody, clone OX-17 does not cross-react with rat RT1B or mouse I-E antigen (Fukumoto et al. 1982).

Mouse anti Rat MHC Class II RT1D antibody, clone OX-17 is routinely tested in flow cytometry on rat splenocytes.

Product Details

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Rat spleen glycoproteins.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized mice were fused with cells from the mouse X63.Ag8.653 myeloma cell line .

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of MHC Class II RT1D antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA1209 F 0.1 mg loader

Application Based External Images

Flow Cytometry

Product Specific References

References for MHC Class II RT1D antibody

  1. Fukumoto, T. et al. (1982) Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus.
    Eur J Immunol. 12 (3): 237-43.
  2. Romaniuk, A. et al. (1995) Rejection of cartilage formed by transplanted allogeneic chondrocytes: evaluation with monoclonal antibodies.
    Transpl Immunol. 3 (3): 251-7.
  3. Volovitz, I. et al. (2011) Split immunity: immune inhibition of rat gliomas by subcutaneous exposure to unmodified live tumor cells.
    J Immunol. 187: 5452-62.
  4. Zeinstra, E. et al. (2006) Simvastatin inhibits interferon-gamma-induced MHC class II up-regulation in cultured astrocytes.
    J Neuroinflammation. 3:16.
  5. Milićević, N.M. et al. (2005) T cells are required for the peripheral phase of B-cell maturation.
    Immunology. 116: 308-17.
  6. Wildner, G. and Diedrichs-Möhring, M. (2003) Autoimmune uveitis induced by molecular mimicry of peptides from rotavirus, bovine casein and retinal S-antigen.
    Eur J Immunol. 33: 2577-87.
  7. Sawa, K. and Mochizuki, M. (1997) Effects of bucillamine and antigen-presenting cells in experimental autoimmune uveitis in rats.
    Jpn J Ophthalmol. 41: 388-95.
  8. Gurbuxani, S. et al. (2001) Selective depletion of inducible HSP70 enhances immunogenicity of rat colon cancer cells.
    Oncogene. 20: 7478-85.
  9. Volovitz, I. et al. (2010) T cell vaccination induces the elimination of EAE effector T cells: analysis using GFP-transduced, encephalitogenic T cells.
    J Autoimmun. 35: 135-44.
  10. Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and leukocyte infiltration in the transgenic rat model of tauopathy.
    J Neuroimmunol. 209: 16-25.
  11. Ghiringhelli, F. et al. (2005) Tumor cells convert immature myeloid dendritic cells into TGF-beta-secreting cells inducing CD4+CD25+ regulatory T cell proliferatio
    J Exp Med. 202: 919-29.

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