MHC Class II RT1D antibody | OX-17

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Mouse anti Rat MHC Class II RT1D

Product Type
Monoclonal Antibody
Clone
OX-17
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA50GA C F 0.1 mg
MCA50R C F 0.25 mg
Mouse anti Rat MHC Class II RT1D antibody, clone OX-17 recognizes a monomorphic determinant on rat RT1D, the rat homologue of mouse I-E, present on all rat strains.

Mouse anti Rat MHC Class II RT1D antibody, clone OX-17 does not cross-react with rat RT1B or mouse I-E antigen (Fukumoto et al. 1982).

Mouse anti Rat MHC Class II RT1D antibody, clone OX-17 is routinely tested in flow cytometry on rat splenocytes.

Product Details

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Rat spleen glycoproteins
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells from the mouse X63.Ag8.653 myeloma cell line .

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of MHC Class II RT1D antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA1209 0.1 mg F

Application Based External Images

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

Product Specific References

References for MHC Class II RT1D antibody

  1. Fukumoto, T. et al. (1982) Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus.
    Eur. J. Immunol. 12: 237-243.
  2. Romaniuk, A. et al. (1995) Rejection of cartilage formed by transplanted allogeneic chondrocytes: evaluation with monoclonal antibodies.
    Transplant. Immunol 3: 251-257.
  3. Volovitz, I. et al. (2011) Split immunity: immune inhibition of rat gliomas by subcutaneous exposure to unmodified live tumor cells.
    J Immunol. 187: 5452-62.
  4. Zeinstra, E. et al. (2006) Simvastatin inhibits interferon-gamma-induced MHC class II up-regulation in cultured astrocytes.
    J Neuroinflammation. 3:16.
  5. Milićević, N.M. et al. (2005) T cells are required for the peripheral phase of B-cell maturation.
    Immunology. 116: 308-17.
  6. Wildner, G. and Diedrichs-Möhring, M. (2003) Autoimmune uveitis induced by molecular mimicry of peptides from rotavirus, bovine casein and retinal S-antigen.
    Eur J Immunol. 33: 2577-87.
  7. Sawa, K. and Mochizuki, M. (1997) Effects of bucillamine and antigen-presenting cells in experimental autoimmune uveitis in rats.
    Jpn J Ophthalmol. 41: 388-95.
  8. Gurbuxani, S. et al. (2001) Selective depletion of inducible HSP70 enhances immunogenicity of rat colon cancer cells.
    Oncogene. 20: 7478-85.
  9. Volovitz, I. et al. (2010) T cell vaccination induces the elimination of EAE effector T cells: analysis using GFP-transduced, encephalitogenic T cells.
    J Autoimmun. 35: 135-44.
  10. Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and leukocyte infiltration in the transgenic rat model of tauopathy.
    J Neuroimmunol. 209: 16-25.
  11. Ghiringhelli, F. et al. (2005) Tumor cells convert immature myeloid dendritic cells into TGF-beta-secreting cells inducing CD4+CD25+ regulatory T cell proliferatio
    J Exp Med. 202: 919-29.