MHC Class II I-Ab antibody | P7/7

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Rat anti Rat MHC Class II I-Ab

Product Type
Monoclonal Antibody
Clone
P7/7
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA09
Datasheet
C F
MSDS
5 ml

Rat anti Rat MHC Class II I-Ab antibody, clone P7/7 recognizes the rat RT1Bu/Du MHC class II antigen and is cross reactive with haplotypes c, d, l, and n. The antibody positively stains MHC class II molecules in rat strains including PVG, F334, LEW, and BN.

Clone P7/7 also recognises mouse MHC Class II, binding to a monomorphic epitope expressed by all mouse strains.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Cells from R8 strain rat
Fusion Partners
Spleen cells from an immunised DA rat were fused with cells of the mouse P3/NSI/I-Ag4.1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of MHC Class II I-Ab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/4
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own sytem using negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Application Based External Images

Flow Cytometry

Product Specific References

References for MHC Class II I-Ab antibody

  1. Momburg, F. et al. (1986) Differential expression of Ia and Ia-associated invariant chain in mouse tissues after in vivo treatment with IFN-gamma.
    J Immunol. 136 (3): 940-8.
  2. Jiang, H.R. et al (2003) Antigen-specific inhibition of experimental autoimmune uveoretinitis by bone marrow-derived immature dendritic cells.
    Invest Ophthalmol Vis Sci. 44: 1598-607.
  3. Taylor, N. et al. (2005) Enhanced tolerance to autoimmune uveitis in CD200-deficient mice correlates with a pronounced Th2 switch in response to antigen challenge.
    J Immunol. 174: 143-54.
  4. Krasinskas, A.M. et al. (2000) Replacement of graft-resident donor-type antigen presenting cells alters the tempo and pathogenesis of murine cardiac allograft rejection.
    Transplantation. 70: 514-21.
  5. Jiang, H.R. et al. (2002) Secretion of interleukin-10 or interleukin-12 by LPS-activated dendritic cells is critically dependent on time of stimulus relative to initiation of purified DC culture.
    J Leukoc Biol. 72: 978-85.
  6. Jiang, H.R. et al. (2006) Combination of vaccination and chimeric receptor expressing T cells provides improved active therapy of tumors.
    J Immunol. 177: 4288-98.