MHC Class II I-Ab antibody | P7/7
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Rat anti Rat MHC Class II I-Ab antibody, clone P7/7 recognizes the rat RT1Bu/Du MHC class II antigen and is cross reactive with haplotypes c, d, l, and n. The antibody positively stains MHC class II molecules in rat strains including PVG, F334, LEW, and BN.
Clone P7/7 also recognises mouse MHC Class II, binding to a monomorphic epitope expressed by all mouse strains.
Rat anti Rat MHC Class II I-Ab antibody, clone P7/7 has been used extensively in protocols used for the isolation of murine dendritic cells from mouse bone marrow by immunomagnetic depletion (Mölzer 2019, Siepmann 2007).
Clone P7/7 also recognises mouse MHC Class II, binding to a monomorphic epitope expressed by all mouse strains.
Rat anti Rat MHC Class II I-Ab antibody, clone P7/7 has been used extensively in protocols used for the isolation of murine dendritic cells from mouse bone marrow by immunomagnetic depletion (Mölzer 2019, Siepmann 2007).
Product Details
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
0.1% Sodium Azide - Immunogen
- Cells from R8 strain rat
- Fusion Partners
- Spleen cells from an immunised DA rat were fused with cells of the mouse P3/NSI/I-Ag4.1 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of MHC Class II I-Ab antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/4 | |
| Immunohistology - Frozen |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own sytem using negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
|
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| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
|
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| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
|
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| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
|
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| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
|
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| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
|
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| Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) | STAR71D650 | F IF | 0.1 mg |
|
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| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
|
|
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
|
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| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
|
Product Specific References
References for MHC Class II I-Ab antibody
-
Momburg, F. et al. (1986) Differential expression of Ia and Ia-associated invariant chain in mouse tissues after in vivo treatment with IFN-gamma.
J Immunol. 136 (3): 940-8. -
Jiang, H.R. et al (2003) Antigen-specific inhibition of experimental autoimmune uveoretinitis by bone marrow-derived immature dendritic cells.
Invest Ophthalmol Vis Sci. 44: 1598-607. -
Taylor, N. et al. (2005) Enhanced tolerance to autoimmune uveitis in CD200-deficient mice correlates with a pronounced Th2 switch in response to antigen challenge.
J Immunol. 174: 143-54. -
Krasinskas, A.M. et al. (2000) Replacement of graft-resident donor-type antigen presenting cells alters the tempo and pathogenesis of murine cardiac allograft rejection.
Transplantation. 70: 514-21. -
Jiang, H.R. et al. (2002) Secretion of interleukin-10 or interleukin-12 by LPS-activated dendritic cells is critically dependent on time of stimulus relative to initiation of purified DC culture.
J Leukoc Biol. 72: 978-85. -
Jiang, H.R. et al. (2006) Combination of vaccination and chimeric receptor expressing T cells provides improved active therapy of tumors.
J Immunol. 177: 4288-98. -
Liu, Y.H. et al. (2018) Partial retinal photoreceptor loss in a transgenic mouse model associated with reduced levels of interphotoreceptor retinol binding protein (IRBP, RBP3).
Exp Eye Res. 172: 54-65. -
Mölzer, C. et al. (2019) TGF-β1-activated type 2 dendritic cells promote wound healing and induce fibroblasts to express tenascin c following corneal full-thickness hydrogel transplantation.
J Tissue Eng Regen Med. 13 (9): 1507-17. -
Christofi, M. et al. (2020) Low-dose 2-deoxy glucose stabilises tolerogenic dendritic cells and generates potent in vivo immunosuppressive effects.
Cell Mol Life Sci. Oct 19 [Epub ahead of print]. -
Siepmann, K. et al. (2007) CD4+CD25+ T regulatory cells induced by LPS-activated bone marrow dendritic cells suppress experimental autoimmune uveoretinitis in vivo.
Graefes Arch Clin Exp Ophthalmol. 245 (2): 221-9. -
Mölzer, C. et al. (2019) Activation of dendritic cells by crosslinked collagen hydrogels (artificial corneas) varies with their composition.
J Tissue Eng Regen Med. 13 (9): 1528-43.
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