MHC Class II I-Ab antibody | P7/7

100% Secure

Rat anti Rat MHC Class II I-Ab

Product Type
Monoclonal Antibody
Clone
P7/7
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA09
Datasheet
C F
SDS
5 ml loader

Rat anti Rat MHC Class II I-Ab antibody, clone P7/7 recognizes the rat RT1Bu/Du MHC class II antigen and is cross reactive with haplotypes c, d, l, and n. The antibody positively stains MHC class II molecules in rat strains including PVG, F334, LEW, and BN.

Clone P7/7 also recognises mouse MHC Class II, binding to a monomorphic epitope expressed by all mouse strains.

Rat anti Rat MHC Class II I-Ab antibody, clone P7/7 has been used extensively in protocols used for the isolation of murine dendritic cells from mouse bone marrow by immunomagnetic depletion (Mölzer 2019, Siepmann 2007).

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Cells from R8 strain rat
Fusion Partners
Spleen cells from an immunised DA rat were fused with cells of the mouse P3/NSI/I-Ag4.1 myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of MHC Class II I-Ab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/4
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own sytem using negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Application Based External Images

Flow Cytometry

Product Specific References

References for MHC Class II I-Ab antibody

  1. Momburg, F. et al. (1986) Differential expression of Ia and Ia-associated invariant chain in mouse tissues after in vivo treatment with IFN-gamma.
    J Immunol. 136 (3): 940-8.
  2. Jiang, H.R. et al (2003) Antigen-specific inhibition of experimental autoimmune uveoretinitis by bone marrow-derived immature dendritic cells.
    Invest Ophthalmol Vis Sci. 44: 1598-607.
  3. Taylor, N. et al. (2005) Enhanced tolerance to autoimmune uveitis in CD200-deficient mice correlates with a pronounced Th2 switch in response to antigen challenge.
    J Immunol. 174: 143-54.
  4. Krasinskas, A.M. et al. (2000) Replacement of graft-resident donor-type antigen presenting cells alters the tempo and pathogenesis of murine cardiac allograft rejection.
    Transplantation. 70: 514-21.
  5. Jiang, H.R. et al. (2002) Secretion of interleukin-10 or interleukin-12 by LPS-activated dendritic cells is critically dependent on time of stimulus relative to initiation of purified DC culture.
    J Leukoc Biol. 72: 978-85.
  6. Jiang, H.R. et al. (2006) Combination of vaccination and chimeric receptor expressing T cells provides improved active therapy of tumors.
    J Immunol. 177: 4288-98.
  7. Liu, Y.H. et al. (2018) Partial retinal photoreceptor loss in a transgenic mouse model associated with reduced levels of interphotoreceptor retinol binding protein (IRBP, RBP3).
    Exp Eye Res. 172: 54-65.
  8. Mölzer, C. et al. (2019) TGF-β1-activated type 2 dendritic cells promote wound healing and induce fibroblasts to express tenascin c following corneal full-thickness hydrogel transplantation.
    J Tissue Eng Regen Med. 13 (9): 1507-17.
  9. Christofi, M. et al. (2020) Low-dose 2-deoxy glucose stabilises tolerogenic dendritic cells and generates potent in vivo immunosuppressive effects.
    Cell Mol Life Sci. Oct 19 [Epub ahead of print].
  10. Siepmann, K. et al. (2007) CD4+CD25+ T regulatory cells induced by LPS-activated bone marrow dendritic cells suppress experimental autoimmune uveoretinitis in vivo.
    Graefes Arch Clin Exp Ophthalmol. 245 (2): 221-9.
  11. Mölzer, C. et al. (2019) Activation of dendritic cells by crosslinked collagen hydrogels (artificial corneas) varies with their composition.
    J Tissue Eng Regen Med. 13 (9): 1528-43.

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra