MHC Class II H-2I-Ak/s antibody | OX-6

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Mouse anti MHC Class II H-2I-Ak/s

Product Type
Monoclonal Antibody
Clone
OX-6
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2687GA
Datasheet
C F P*
SDS
0.1 mg loader

MCA2687R
Datasheet
C F P* WB
SDS
0.25 mg loader

Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 recognizes a monomorphic determinant of the rat RT1B MHC class II antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells.

Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 does not react with the rat BDIX strain due to a defect in RT1B expression (Male et al. 1987).

Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 also cross reacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region (McMaster & Williams 1979) and (Male et al. 1987).

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the H-2 region. In rats, this complex is referred to as the RT1 region.

Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 is routinely tested in flow cytometry on rat splenocytes.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Rat thymocyte membrane glycoproteins.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells from the NS1 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of MHC Class II H-2I-Ak/s antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunohistology - Paraffin 1 1/50 1/100
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose. PLP fixation is recommended for optimal results
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA1209 F 0.1 mg loader

Application Based External Images

Flow Cytometry

Product Specific References

References for MHC Class II H-2I-Ak/s antibody

  1. McMaster, W.R. & Williams, A.F. (1979) Identification of Ia glycoproteins in rat thymus and purification from rat spleen.
    Eur J Immunol. 9 (6): 426-33.
  2. Fernandez, J.L. & Weeks, M. (1986) Genetic monitoring of inbred strains of mice using monoclonal antibodies to major histocompatibility haplotypes and lymphocyte alloantigens.
    Lab Anim. 20 (4): 293-7.
  3. Charteris, D.G. & Lightman, S.L. (1993) In vivo lymphokine production in experimental autoimmune uveoretinitis.
    Immunology. 78 (3): 387-92.
  4. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
    J Histochem Cytochem. 43 (3): 313-20.
  5. McKechnie, N.M. et al. (1997) Immunization with the cross-reactive antigens Ov39 from Onchocerca volvulus and hr44 from human retinal tissue induces ocular pathology and activates retinal microglia.
    J Infect Dis. 176 (5): 1334-43.
  6. Male, D.K. et al. (1987) Serological evidence for a defect in RT1.B (I-A) expression by the BDIX rat strain.
    J Immunogenet. 14 (6): 301-12.
  7. Meyer zu Hörste, G. et al. (2011) Quinpramine ameliorates rat experimental autoimmune neuritis and redistributes MHC class II molecules.
    PLoS One. 6(6): e21223.
  8. Zhang, M. et al. (2015) The distinct distributions of immunocompetent cells in rat dentin pulp after pulpotomy.
    Anat Rec (Hoboken). 298 (4): 741-9.
  9. Ledreux, A. et al. (2016) Detrimental effects of a high fat/high cholesterol diet on memory and hippocampal markers in aged rats
    Behavioural Brain Research. Jun 22 [Epub ahead of print]