MHC Class II H-2I-Ak/s antibody | OX-6
Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 does not react with the rat BDIX strain due to a defect in RT1B expression (Male et al. 1987).
Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 also cross reacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region (McMaster & Williams 1979) and (Male et al. 1987).
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the H-2 region. In rats, this complex is referred to as the RT1 region.
Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 is routinely tested in flow cytometry on rat splenocytes.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Rat thymocyte membrane glycoproteins.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells from the NS1 mouse myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- For research purposes only
Applications of MHC Class II H-2I-Ak/s antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Immunohistology - Paraffin 1||1/50||1/100|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose. PLP fixation is recommended for optimal results
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Secondary Antibodies Available
Negative Isotype Controls Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Mouse IgG1 Negative Control||MCA1209||F||0.1 mg|
Product Specific References
References for MHC Class II H-2I-Ak/s antibody
McMaster, W.R. & Williams, A.F. (1979) Identification of Ia glycoproteins in rat thymus and purification from rat spleen.
Eur J Immunol. 9 (6): 426-33.
Fernandez, J.L. & Weeks, M. (1986) Genetic monitoring of inbred strains of mice using monoclonal antibodies to major histocompatibility haplotypes and lymphocyte alloantigens.
Lab Anim. 20 (4): 293-7.
Charteris, D.G. & Lightman, S.L. (1993) In vivo lymphokine production in experimental autoimmune uveoretinitis.
Immunology. 78 (3): 387-92.
Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
J Histochem Cytochem. 43 (3): 313-20.
McKechnie, N.M. et al. (1997) Immunization with the cross-reactive antigens Ov39 from Onchocerca volvulus and hr44 from human retinal tissue induces ocular pathology and activates retinal microglia.
J Infect Dis. 176 (5): 1334-43.
Male, D.K. et al. (1987) Serological evidence for a defect in RT1.B (I-A) expression by the BDIX rat strain.
J Immunogenet. 14 (6): 301-12.
Meyer zu Hörste, G. et al. (2011) Quinpramine ameliorates rat experimental autoimmune neuritis and redistributes MHC class II molecules.
PLoS One. 6(6): e21223.
Zhang, M. et al. (2015) The distinct distributions of immunocompetent cells in rat dentin pulp after pulpotomy.
Anat Rec (Hoboken). 298 (4): 741-9.
Ledreux, A. et al. (2016) Detrimental effects of a high fat/high cholesterol diet on memory and hippocampal markers in aged rats
Behavioural Brain Research. Jun 22 [Epub ahead of print]