IgG2a antibody | AbD09345

Human anti Rat IgG2a

Product Type
Monoclonal Antibody
Clone
AbD09345
Isotype
HuCAL Fab bivalent
Specificity
IgG2a

Product Code Applications Pack Size List Price Your Price Qty
HCA120
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
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loader
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Human anti Rat IgG2a antibody, clone AbD09345 recognizes Rat IgG2a. No cross reactivity is seen with other rat immunoglobulin subclasses.

Target Species
Rat
Product Form
A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
Preparation
StrepTactin affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Purified Rat monoclonal IgG2a kappa.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/20 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

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RRID
AB_2021049
UniProt
P20760
Entrez Gene
LOC679045
GO Terms
GO:0003823 antigen binding
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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