IgG2a antibody | AbD09345
Human anti Rat IgG2a antibody, clone AbD09345 recognizes Rat IgG2a.
No cross reactivity is seen with other rat immunoglobulin subclasses.
Product Details
- Target Species
- Rat
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilized.
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- StrepTactin affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Purified Rat monoclonal IgG2a kappa.
- Approx. Protein Concentrations
- Total protein concentration 0.1 mg/ml after reconstitution
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P20760
- Entrez Gene
- LOC679045
- GO Terms
- GO:0003823 antigen binding
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
- Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
- Regulatory
- For research purposes only
Applications of IgG2a antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/20 | 1/200 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product is suitable for use in direct ELISA applications. Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti c-Myc:Alk.Phos. | MCA2200A | C E P WB* | 0.1 mg |
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| Mouse anti c-Myc:Biotin | MCA2200B | C E P WB* | 0.1 mg |
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| Mouse anti c-Myc:HRP | MCA2200P | C E P WB* | 0.1 mg |
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| Mouse anti Strep-Tag Classic:HRP | MCA2489P | E WB | 75 µl |
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