IgG2a antibody | AbD09345

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Human anti Rat IgG2a

Product Type
Monoclonal Antibody
Clone
AbD09345
Isotype
HuCAL Fab bivalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA120 E 0.1 mg
Human anti Rat IgG2a antibody, clone AbD09345 recognizes Rat IgG2a. No cross reactivity is seen with other rat immunoglobulin subclasses.

Product Details

Target Species
Rat
Product Form
A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilized.
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
StrepTactin affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Purified Rat monoclonal IgG2a kappa
Approx. Protein Concentrations
Total protein concentration 0.1 mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life
12 months from date of reconstitution

More Information

UniProt
P20760 Related reagents
Entrez Gene
LOC679045 Related reagents
GO Terms
GO:0003823 antigen binding
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of IgG2a antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/20 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product is suitable for use in direct ELISA applications. Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti c-Myc:Alk.Phos. MCA2200A 0.1 mg C E P WB*
Mouse anti c-Myc:Biotin MCA2200B 0.1 mg C E P WB*
Mouse anti c-Myc:HRP MCA2200P 0.1 mg C E P WB*
Mouse anti Strep-Tag Classic:HRP MCA2489P 75 µl E WB