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IgG1 antibody | MARG1-2

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Mouse anti Rat IgG1 Heavy Chain:Biotin

Product Type
Monoclonal Antibody
Clone
MARG1-2
Isotype
IgG1
Specificity
IgG1
Region
HEAVY CHAIN

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Product Code Applications Pack Size List Price Your Price Qty
MCA194B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.5 mg loader
List Price Your Price
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Mouse anti Rat IgG1, clone MARG1-2 recognizes the heavy chain of the rat immunoglobulin G1 subclass. No cross-reactivity has been found with other rat immunoglobulin classes or subclasses.

Target Species
Rat
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
50%Glycerol
Immunogen
Rat IR27 IgG1 myeloma protein.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 500ng/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

References for IgG1 antibody

  1. Pelegrí, C. et al. (2001) Prevention of adjuvant arthritis by the W3/25 anti-CD4 monoclonal antibody is associated with a decrease of blood CD4(+)CD45RC(high) T cells.
    Clin Exp Immunol. 125 (3): 470-7.
  2. Sato, K. et al. (2001) Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat cardiac transplants.
    J Immunol. 166 (6): 4185-94.
  3. Bézie, S. et al. (2015) Fibrinogen-Like Protein 2/Fibroleukin Induces Long-Term Allograft Survival in a Rat Model through Regulatory B Cells.
    PLoS One. 10 (3): e0119686.
  4. Bézie, S. et al. (2015) Compensatory Regulatory Networks between CD8 T, B, and Myeloid Cells in Organ Transplantation Tolerance.
    J Immunol. 195 (12): 5805-15.
  5. Ueta, H. et al. (2018) Single blood transfusion induces the production of donor-specific alloantibodies and regulatory T cells mainly in the spleen.
    Int Immunol. 30 (2): 53-67.

RRID
AB_321808
UniProt
P20759
Entrez Gene
Ighg
GO Terms
GO:0003823 antigen binding

MCA194B

152459 170920

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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