Endothelium antibody | OX-43

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Mouse anti Rat Endothelium

Product Type
Monoclonal Antibody
Clone
OX-43
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA276GA C F IP 0.1 mg
MCA276R C F IP 0.25 mg
Mouse anti Rat Endothelium antibody, clone OX-43 recognizes an antigen of ~90 kDa expressed on all vascular endothelial cells in the rat, with the exception of those in the brain. Mouse anti Rat Endothelium antibody, clone OX-43 also recognizes resident and activated peritoneal macrophages, a subset of alveolar macrophages and erythrocytes.

Product Details

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Rat peritoneal macrophages.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with the NS0/U mouse myeloma cell line.

Storage Information

Storage
Pack Size: 0.1 mg
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 0.25 mg
Store at +4oC or at -20oC if preferred

This product should be stored undiluted..

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Endothelium antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA1209 0.1 mg F

Product Specific References

References for Endothelium antibody

  1. Robinson, A.P. et al. (1986) MRC OX-43: a monoclonal antibody which reacts with all vascular endothelium in the rat except that of brain capillaries.
    Immunology. 57 (2): 231-7.
  2. Barclay, A.N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
    Immunology. 42 (4): 593-600.
  3. Ulger, H. et al. (2002) Labelling of rat endothelial cells with antibodies to vWF, RECA-1, PECAM-1, ICAM-1, OX-43 and ZO-1.
    Anat Histol Embryol. 31 (1): 31-5.
  4. Fiegel, H.C. et al. (2003) Characterization of cell types during rat liver development.
    Hepatology. 37 (1): 148-54.
  5. Fiegel, H.C. et al. (2003) Hepatic lineages isolated from developing rat liver show different ways of maturation.
    Biochem Biophys Res Commun. 305 (1): 46-53.
  6. Lange, C. et al. (2006) Hepatocytic differentiation of mesenchymal stem cells in cocultures with fetal liver cells.
    World J Gastroenterol. 12 (15): 2394-7.
  7. Mittal, B. et al. (2003) Expression of a membrane-bound form of the ferroxidase ceruloplasmin by leptomeningeal cells.
    Glia. 41 (4): 337-46.
  8. Koenig, S. et al. (2005) Liver repopulation after hepatocellular transplantation: integration and interaction of transplanted hepatocytes in the host.
    Cell Transplant. 14 (1): 31-40.
  9. Metzger, R. et al. (2011) Heterogeneous distribution of angiotensin I-converting enzyme (CD143) in the human and rat vascular systems: vessel, organ and species specificity.
    Microvasc Res. 81 (2): 206-15.