Mouse anti Rat Endothelium antibody, clone OX-43 recognizes an antigen of ~90 kDa expressed on all vascular endothelial cells in the rat, with the exception of those in the brain. Mouse anti Rat Endothelium antibody, clone OX-43 also recognizes resident and activated peritoneal macrophages, a subset of alveolar macrophages and erythrocytes.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- Rat peritoneal macrophages.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with the NS0/U mouse myeloma cell line.
Pack Size: 0.1 mg
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 0.25 mg
Store at +4oC or at -20oC if preferred
This product should be stored undiluted..
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Endothelium antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
References for Endothelium antibody
Robinson, A.P. et al. (1986) MRC OX-43: a monoclonal antibody which reacts with all vascular endothelium in the rat except that of brain capillaries.
Immunology. 57 (2): 231-7.
Barclay, A.N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
Immunology. 42 (4): 593-600.
Ulger, H. et al. (2002) Labelling of rat endothelial cells with antibodies to vWF, RECA-1, PECAM-1, ICAM-1, OX-43 and ZO-1.
Anat Histol Embryol. 31 (1): 31-5.
Fiegel, H.C. et al. (2003) Characterization of cell types during rat liver development.
Hepatology. 37 (1): 148-54.
Fiegel, H.C. et al. (2003) Hepatic lineages isolated from developing rat liver show different ways of maturation.
Biochem Biophys Res Commun. 305 (1): 46-53.
Lange, C. et al. (2006) Hepatocytic differentiation of mesenchymal stem cells in cocultures with fetal liver cells.
World J Gastroenterol. 12 (15): 2394-7.
Mittal, B. et al. (2003) Expression of a membrane-bound form of the ferroxidase ceruloplasmin by leptomeningeal cells.
Glia. 41 (4): 337-46.
Koenig, S. et al. (2005) Liver repopulation after hepatocellular transplantation: integration and interaction of transplanted hepatocytes in the host.
Cell Transplant. 14 (1): 31-40.
Metzger, R. et al. (2011) Heterogeneous distribution of angiotensin I-converting enzyme (CD143) in the human and rat vascular systems: vessel, organ and species specificity.
Microvasc Res. 81 (2): 206-15.