ED5 Antigen antibody | ED5

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Mouse anti Rat Follicular Dendritic Cells

Product Type
Monoclonal Antibody
Clone
ED5
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA530R
Datasheet
C
MSDS
0.25 mg loader

Mouse anti rat follicular dendritic cell antibody, clone ED5 recognizes the ED5 antigen, revealing a web-like pattern in B cell follicles, representing the follicular dendritic cells. Mesangial cells in the kidney are also ED5-positive.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Rat spleen cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the Sp2/0 - Ag14 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC for one month or at -20oC for longer.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of ED5 Antigen antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/100 1/200
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recomended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for ED5 Antigen antibody

  1. Jeurissen, S.H. & Dijkstra, C.D. (1986) Characteristics and functional aspects of nonlymphoid cells in rat germinal centers, recognized by two monoclonal antibodies ED5 and ED6.
    Eur J Immunol. 16 (5): 562-8.
  2. Kapasi, Z.F. (1998) Follicular dendritic cell (FDC) precursors in primary lymphoid tissues.
    J Immunol. 160: 1078-84.
  3. Yamate, J. et al. (2002) Establishment and characterization of transplantable tumor line (KB) and cell lines (KB-P and KB-D8) from a rat thymus-derived dendritic cell sarcoma.
    Virchows Arch. 440: 616-26.
  4. Köhler, C.N. (2010) The actin-binding protein caldesmon is in spleen and lymph nodes predominately expressed by smooth-muscle cells, reticular cells, and follicular dendritic cells.
    J Histochem Cytochem. 58 (2): 183-93.
  5. Ohmori, Y. et al. (2001) Repeated Antigen Challenge Modulates Expression of Follicular Dendritic Cell (FDC) Related Molecule in Draining Lymph Nodes
    Acta Histochemica et Cytochemica 34: 265-73.
  6. Zandvoort, A. et al. (2001) Slow recovery of follicular B cells and marginal zone B cells after chemotherapy: implications for humoral immunity.
    Clin Exp Immunol. 124 (2): 172-9.
  7. Camelo, S. et al. (2004) Capture of intracamerally placed Ag by resident macrophages of the spleen and of the cervical and mesenteric lymph nodes.
    ARVO Annual Meeting Abstract 2406157.