ED5 Antigen antibody | ED5
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Mouse anti Rat Follicular Dendritic Cells
- Product Type
- Monoclonal Antibody
- Clone
- ED5
- Isotype
- IgG1
- Specificity
- ED5 Antigen
- Region
- (FOLLICULAR DENDRITIC CELLS)
Antibody Quality and 
Unrivaled 
| Mouse anti rat follicular dendritic cell antibody, clone ED5 recognizes the ED5 antigen, revealing a web-like pattern in B cell follicles, representing the follicular dendritic cells. Mesangial cells in the kidney are also ED5-positive. |
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Rat spleen cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the Sp2/0 - Ag14 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen |  |
1/100 | 1/200 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recomended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for ED5 Antigen antibody
-
Jeurissen, S.H. & Dijkstra, C.D. (1986) Characteristics and functional aspects of nonlymphoid cells in rat germinal centers, recognized by two monoclonal antibodies ED5 and ED6.
Eur J Immunol. 16 (5): 562-8. -
Kapasi, Z.F. (1998) Follicular dendritic cell (FDC) precursors in primary lymphoid tissues.
J Immunol. 160: 1078-84. -
Yamate, J. et al. (2002) Establishment and characterization of transplantable tumor line (KB) and cell lines (KB-P and KB-D8) from a rat thymus-derived dendritic cell sarcoma.
Virchows Arch. 440: 616-26. -
Köhler, C.N. (2010) The actin-binding protein caldesmon is in spleen and lymph nodes predominately expressed by smooth-muscle cells, reticular cells, and follicular dendritic cells.
J Histochem Cytochem. 58 (2): 183-93. -
Ohmori, Y. et al. (2001) Repeated Antigen Challenge Modulates Expression of Follicular Dendritic Cell (FDC) Related Molecule in Draining Lymph Nodes
Acta Histochemica et Cytochemica 34: 265-73. -
Zandvoort, A. et al. (2001) Slow recovery of follicular B cells and marginal zone B cells after chemotherapy: implications for humoral immunity.
Clin Exp Immunol. 124 (2): 172-9. -
Camelo, S. et al. (2004) Capture of intracamerally placed Ag by resident macrophages of the spleen and of the cervical and mesenteric lymph nodes.
ARVO Annual Meeting Abstract 2406157.
- RRID
- AB_322058
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