ED5 Antigen antibody | ED5

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Mouse anti Rat Follicular Dendritic Cells

Product Type: Monoclonal Antibody
Clone: ED5
Isotype: IgG1
Specificity: ED5 Antigen
Region: (FOLLICULAR DENDRITIC CELLS)

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Secondary Antibodies
References (7)
Applications
Storage Information

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MCA530R Datasheet Datasheet SDS SDS	C	0.25 mg		Log in	Get Quote
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Mouse anti rat follicular dendritic cell antibody, clone ED5 recognizes the ED5 antigen, revealing a web-like pattern in B cell follicles, representing the follicular dendritic cells. Mesangial cells in the kidney are also ED5-positive.

Target Species: Rat
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Antibody purified from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
Carrier Free: Yes
Immunogen: Rat spleen cells.
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the Sp2/0 - Ag14 mouse myeloma cell line.
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Target Species	Cross Reactivity
Mouse

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Frozen		1/100	1/200

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recomended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for ED5 Antigen antibody

Jeurissen, S.H. & Dijkstra, C.D. (1986) Characteristics and functional aspects of nonlymphoid cells in rat germinal centers, recognized by two monoclonal antibodies ED5 and ED6.
Eur J Immunol. 16 (5): 562-8.
Kapasi, Z.F. (1998) Follicular dendritic cell (FDC) precursors in primary lymphoid tissues.
J Immunol. 160: 1078-84.
Yamate, J. et al. (2002) Establishment and characterization of transplantable tumor line (KB) and cell lines (KB-P and KB-D8) from a rat thymus-derived dendritic cell sarcoma.
Virchows Arch. 440: 616-26.
Köhler, C.N. (2010) The actin-binding protein caldesmon is in spleen and lymph nodes predominately expressed by smooth-muscle cells, reticular cells, and follicular dendritic cells.
J Histochem Cytochem. 58 (2): 183-93.
Ohmori, Y. et al. (2001) Repeated Antigen Challenge Modulates Expression of Follicular Dendritic Cell (FDC) Related Molecule in Draining Lymph Nodes
Acta Histochemica et Cytochemica 34: 265-73.
Zandvoort, A. et al. (2001) Slow recovery of follicular B cells and marginal zone B cells after chemotherapy: implications for humoral immunity.
Clin Exp Immunol. 124 (2): 172-9.
Camelo, S. et al. (2004) Capture of intracamerally placed Ag by resident macrophages of the spleen and of the cervical and mesenteric lymph nodes.
ARVO Annual Meeting Abstract 2406157.