ED5 Antigen antibody | ED5
Mouse anti rat follicular dendritic cell antibody, clone ED5 recognizes the ED5 antigen, revealing a web-like pattern in B cell follicles, representing the follicular dendritic cells. Mesangial cells in the kidney are also ED5-positive.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Rat spleen cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the Sp2/0 - Ag14 mouse myeloma cell line.
- Store at +4oC for one month or at -20oC for longer.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- For research purposes only
Applications of ED5 Antigen antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||1/100||1/200|
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recomended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for ED5 Antigen antibody
Jeurissen, S.H. & Dijkstra, C.D. (1986) Characteristics and functional aspects of nonlymphoid cells in rat germinal centers, recognized by two monoclonal antibodies ED5 and ED6.
Eur J Immunol. 16 (5): 562-8.
Kapasi, Z.F. (1998) Follicular dendritic cell (FDC) precursors in primary lymphoid tissues.
J Immunol. 160: 1078-84.
Yamate, J. et al. (2002) Establishment and characterization of transplantable tumor line (KB) and cell lines (KB-P and KB-D8) from a rat thymus-derived dendritic cell sarcoma.
Virchows Arch. 440: 616-26.
Köhler, C.N. (2010) The actin-binding protein caldesmon is in spleen and lymph nodes predominately expressed by smooth-muscle cells, reticular cells, and follicular dendritic cells.
J Histochem Cytochem. 58 (2): 183-93.
Ohmori, Y. et al. (2001) Repeated Antigen Challenge Modulates Expression of Follicular Dendritic Cell (FDC) Related Molecule in Draining Lymph Nodes
Acta Histochemica et Cytochemica 34: 265-73.
Zandvoort, A. et al. (2001) Slow recovery of follicular B cells and marginal zone B cells after chemotherapy: implications for humoral immunity.
Clin Exp Immunol. 124 (2): 172-9.
Camelo, S. et al. (2004) Capture of intracamerally placed Ag by resident macrophages of the spleen and of the cervical and mesenteric lymph nodes.
ARVO Annual Meeting Abstract 2406157.