Choline Acetyltransferase antibody | 1E6

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Mouse anti Rat Choline Acetyltransferase

Product Type
Monoclonal Antibody
Clone
1E6
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
2080-0101 C 0.1 ml
Mouse anti Rat choline acetyltransferase antibody, clone 1E6 recognizes choline acetyltransferase and stains cholinergic neurons in the brain and central nervous system.

Choline acetyltransferase is an enzyme, present the presynaptic ends of axons, that catalyzes the transfer of the acetyl group of acetyl CoA to choline, forming the neurotransmitter acetylcholine.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chicken
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ascitic Fluid
Preservative Stabilisers
None present.
Immunogen
Choline acetyltransferase purified from rat brain.

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P32738 Related reagents
Entrez Gene
Chat Related reagents
GO Terms
GO:0001547 antral ovarian follicle growth
GO:0001666 response to hypoxia
GO:0004102 choline O-acetyltransferase activity
GO:0005737 cytoplasm
GO:0007584 response to nutrient
GO:0007613 memory
GO:0008292 acetylcholine biosynthetic process
GO:0042493 response to drug
GO:0019717 synaptosome
GO:0033265 choline binding
GO:0045471 response to ethanol
Regulatory
For research purposes only

Applications of Choline Acetyltransferase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/100 1/250
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Product Specific References

References for Choline Acetyltransferase antibody

  1. Hagg, T. et al. (1988) Delayed treatment with nerve growth factor reverses the apparent loss of cholinergic neurons after acute brain damage
    Exp Neurol. 101: 303 - 312
  2. Dietz, G. W., Jr. & Salvaterra, P. M. (1980) Purification and peptide mapping of rat brain choline acetyltransferase
    J. Biol. Chem. 255: 10612 - 10617.
  3. Crawford, G. D., et al. (1982) Interaction of monoclonal antibodies with mammalian choline acetyltransferase.
    Proc Natl Acad Sci U S A. 79: 7031 - 7035.
  4. Matthews, D. A. et al. (1987) An immunocytochemical study of choline acetyltransferase-containing neurons and axon terminals in normal and partially deafferented hippocampal formation
    Brain Res. 402: 30 - 43
  5. Hagg, T. et al. (1989) Nerve growth factor (NGF) reverses axotomy-induced decreases in choline acetyltransferase, NGF receptor and size of medial septum cholinergic neurons
    Brain Res. 505: 29 - 38.
  6. Lindh, B., et al. (1986) Immunohistochemical demonstration of choline acetyltransferase-immunoreactive preganglionic nerve fibers in guinea pig autonomic ganglia.
    Proc Natl Acad Sci U S A.83: 5316 - 5320.
  7. Hagg, T. et al. (1992) Ciliary neurotrophic factor prevents neuronal degeneration and promotes low affinity NGF receptor expression in the adult rat CNS
    Neuron 8: 145-158.