CD63 antibody | AD1
Mouse anti Rat CD63 antibody 6H1 (MCA4754GA) used to demonstrate CD63 expressing cells by immunofluorescence.
Image caption:
Glucolipotoxicity leads to mTOR-dependent inhibition of macroautophagy in β cells. a Immunofluorescence of CD63 (green) and mTOR (red) in INS1 cells treated with control (1% BSA, 11 mM glucose, “1% BSA”) or glucolipotoxic (1% BSA, 33.3 mM Glucose and 0.4 mM Palmitate, “Glc/Pal”) media for 20 h. Golgi-CFP was used to visualize the Golgi apparatus. Arrowheads point to large CD63/mTOR puncta. Scale bar, 5 μm; for inset: 2 μm.
From: Pasquier, A. et al.
Lysosomal degradation of newly formed insulin granules contributes to β cell failure in diabetes.
Nat Commun. 2019 Jul 25;10(1):3312.
doi: 10.1038/s41467-019-11170-4.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat CD63 antibody 6H1 (MCA4754GA) used to demonstrate CD63 expressing cells by immunofluorescence.
Image caption:
Stress-induced nascent granule degradation requires CD63.
IF of (pro)insulin (green) and CD63 (red) in INS1 cells stably expressing shRNA against Prkd1 (shPKD1), transfected with indicated siRNAs for 48 h. Scale bar, 10 μm. Nuclei were stained with DAPI.
From: Pasquier, A. et al.
Lysosomal degradation of newly formed insulin granules contributes to β cell failure in diabetes.
Nat Commun. 2019 Jul 25;10(1):3312.
doi: 10.1038/s41467-019-11170-4.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat CD63:Alexa Fluor®647
- Product Type
- Monoclonal Antibody
- Clone
- AD1
- Isotype
- IgG1
- Specificity
- CD63
Antibody Quality and Performance Guarantee
| Mouse anti Rat CD63 antibody, clone AD1 recognizes rat CD63, an intracellular type-III lysosomal glycoprotein and member of the tetraspanin (TM4SF) family, found on activated platelets and resident macrophages. Rat CD63 is expressed on mast cells in tissues and can be induced on other cell types in vitro (Nishikata et al. 1992). Mouse anti Rat CD63 antibody, clone AD1 partially inhibits IgE-mediated histamine release and binding competition assays indicate that it is sterically situated close to the high-affinity IgE receptor on rat basophilic leukemia (RBL-2H3) cells (Kitani et al. 1991). |
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- RBL-2H3 rat basophilic leukaemia cell line.
- Approx. Protein Concentrations
- IgG concentration 0.05mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the X63-Ag8-653 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA1209A647 | F | 100 Tests/1ml |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control:Alexa Fluor® 647 | ||||||
Source Reference
-
Kitani, S. et al. (1991) A cell surface glycoprotein of rat basophilic leukemia cells close to the high affinity IgE receptor (Fc epsilon RI). Similarity to human melanoma differentiation antigen ME491.
J Biol Chem. 266 (3): 1903-9.
Antibody Characterization Reference
-
Nishikata, H. et al. (1992) The rat mast cell antigen AD1 (homologue to human CD63 or melanoma antigen ME491) is expressed in other cells in culture.
J Immunol. 149(3): 862-70.
References for CD63 antibody
-
Boros, M. et al. (1995) Response of mucosal mast cells to intestinal ischemia-reperfusion injury in the rat.
Shock. 3 (2): 125-31. -
Vincent-Schneider, H. et al. (2001) Secretory granules of mast cells accumulate mature and immature MHC class II molecules
J Cell Sci. 114(Pt 2): 323-34. -
Kraft, S. et al. (2005) Anti-CD63 antibodies suppress IgE-dependent allergic reactions in vitro and in vivo.
J Exp Med. 201: 385-96. -
Ramezani-Moghadam, M. et al. (2015) Adiponectin Reduces Hepatic Stellate Cell Migration by Promoting Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) Secretion.
J Biol Chem. 290(9): 5533-42. -
Yoshimura, A. et al. (2016) Generation of a novel transgenic rat model for tracing extracellular vesicles in body fluids.
Sci Rep. 6: 31172. -
Pasquier, A. et al. (2019) Lysosomal degradation of newly formed insulin granules contributes to β cell failure in diabetes.
Nat Commun. 10 (1): 3312. -
Pusic, K.M. et al. (2019) IFNγ-Stimulated Dendritic Cell Exosomes for Treatment of Migraine Modeled Using Spreading Depression.
Front Neurosci. 13: 942.
- RRID
- AB_10613490
- UniProt
- P28648
- Entrez Gene
- Cd63
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005765 lysosomal membrane
- GO:0030658 transport vesicle membrane
- GO:0010633 negative regulation of epithelial cell migration
- GO:0030855 epithelial cell differentiation
- GO:0032403 protein complex binding
- GO:0043234 protein complex
- GO:0045785 positive regulation of cell adhesion
- View More GO Terms
- GO:0045807 positive regulation of endocytosis
MCA4754A647
157596
260112
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