CD49d antibody | MRalpha4-1
Mouse anti Rat CD49d monoclonal antibody, clone MRalpha4-1 recognizes rat CD49d, otherwise known as integrin alpha 4 chain/VLA-4, a ~150 kDa single pass type-1 transmembrane glycoprotein and member of the integrin alpha chain family, which acts as a receptor for fibronectin and CD106 (VCAM-1).
CD49d is expressed broadly on T and B lymphocytes, monocytes, thymocytes, mast cells and dendritic cells and is involved in cell-cell interactions, mononuclear cell trafficking to sites of endothelial inflammation, and the adhesion of cells to the extracellular matrix.
Clone MRalpha4-1 inhibits the in vitro and in vivo functions of CD49d (Yasuda et al. 1995). Studies have shown that in rat Peyer's patches, clone MRalpha4-1 inhibits the rolling and adherence of lymphocytes to postcapillary venules (PCV), and significantly inhibits the transendothelial migration of T lymphocytes (Tsuzuki et al. 1996).
CD49d is expressed broadly on T and B lymphocytes, monocytes, thymocytes, mast cells and dendritic cells and is involved in cell-cell interactions, mononuclear cell trafficking to sites of endothelial inflammation, and the adhesion of cells to the extracellular matrix.
Clone MRalpha4-1 inhibits the in vitro and in vivo functions of CD49d (Yasuda et al. 1995). Studies have shown that in rat Peyer's patches, clone MRalpha4-1 inhibits the rolling and adherence of lymphocytes to postcapillary venules (PCV), and significantly inhibits the transendothelial migration of T lymphocytes (Tsuzuki et al. 1996).
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- RBL-2H3 rat basophilic leukaemia cell line.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the P3U1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of CD49d antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:FITC | MCA1210F | F | 0.1 mg |
|
Product Specific References
References for CD49d antibody
-
Yasuda, M. et al. (1995) Expression and function of fibronectin binding integrins on rat mast cells.
Int Immunol. 7 (2): 251-8. -
Tsuzuki Y et al. (1996) alpha 4 integrin plays a critical role in early stages of T lymphocyte migration in Peyer's patches of rats.
Int Immunol. 8 (3): 287-95. -
Summers, L. et al. (2013) Activation of brain endothelial cells by interleukin-1 is regulated by the extracellular matrix after acute brain injury.
Mol Cell Neurosci. 57: 93-103.
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