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CD49d antibody | MRalpha4-1

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Anti Rat CD49d Antibody, clone MRalpha4-1 thumbnail image 1 Anti Rat CD49d Antibody, clone MRalpha4-1 thumbnail image 2 Anti Rat CD49d Antibody, clone MRalpha4-1 thumbnail image 3
Anti Rat CD49d Antibody, clone MRalpha4-1 gallery image 1
Figure A. Alexa Fluor 647 conjugated Mouse anti Rat CD45 (MCA43A647) and FITC conjugated Mouse IgG2a isotype control (MCA1210F). Figure B. Alexa Fluor 647 conjugated Mouse anti Rat CD45 (MCA43A647) and FITC conjugated Mouse anti Rat CD49d (MCA2872F). All experiments performed on red cell lysed rat blood gated on lymphoid cells in the presence of 10% rat serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Rat CD49d Antibody, clone MRalpha4-1 gallery image 2
Figure A. FITC conjugated Mouse anti Rat CD45 (MCA43FB) and RPE conjugated Mouse IgG2a isotype control (MCA1210PE). Figure B. FITC conjugated Mouse anti Rat CD45 (MCA43FB) and RPE conjugated Mouse anti Rat CD49d (MCA2872PE). All experiments performed on red cell lysed rat blood gated on mononuclear cells.
Anti Rat CD49d Antibody, clone MRalpha4-1 gallery image 3
Figure A. RPE conjugated Mouse anti Rat CD49d (MCA2872PE) and FITC conjugated Mouse IgG1 isotype control (MCA1209F). Figure B. RPE conjugated Mouse anti Rat CD49d (MCA2872PE) and FITC conjugated Mouse anti Rat CD45 (MCA43FB). All experiments performed on red cell lysed rat blood gated on mononuclear cells.

Mouse anti Rat CD49d:FITC

Product Type
Monoclonal Antibody
Clone
MRalpha4-1
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA2872F
Datasheet
F
SDS
0.1 mg loader

Mouse anti Rat CD49d monoclonal antibody, clone MRalpha4-1 recognizes rat CD49d, otherwise known as integrin alpha 4 chain/VLA-4, a ~150 kDa single pass type-1 transmembrane glycoprotein and member of the integrin alpha chain family, which acts as a receptor for fibronectin and CD106 (VCAM-1).

CD49d is expressed broadly on T and B lymphocytes, monocytes, thymocytes, mast cells and dendritic cells and is involved in cell-cell interactions, mononuclear cell trafficking to sites of endothelial inflammation, and the adhesion of cells to the extracellular matrix.

Clone MRalpha4-1 inhibits the in vitro and in vivo functions of CD49d (Yasuda et al. 1995). Studies have shown that in rat Peyer's patches, clone MRalpha4-1 inhibits the rolling and adherence of lymphocytes to postcapillary venules (PCV), and significantly inhibits the transendothelial migration of T lymphocytes (Tsuzuki et al. 1996).

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
RBL-2H3 rat basophilic leukaemia cell line.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the P3U1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of CD49d antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2a Negative Control:FITC MCA1210F F 0.1 mg loader

Product Specific References

References for CD49d antibody

  1. Yasuda, M. et al. (1995) Expression and function of fibronectin binding integrins on rat mast cells.
    Int Immunol. 7 (2): 251-8.
  2. Tsuzuki Y et al. (1996) alpha 4 integrin plays a critical role in early stages of T lymphocyte migration in Peyer's patches of rats.
    Int Immunol. 8 (3): 287-95.
  3. Summers, L. et al. (2013) Activation of brain endothelial cells by interleukin-1 is regulated by the extracellular matrix after acute brain injury.
    Mol Cell Neurosci. 57: 93-103.

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