Browsing Modes for Business Users

CD45RC antibody | OX-22

ISO 9001 Quality Assurance Manufacturing | Print
Anti Rat CD45RC Antibody, clone OX-22 thumbnail image 1 Anti Rat CD45RC Antibody, clone OX-22 thumbnail image 2 Anti Rat CD45RC Antibody, clone OX-22 thumbnail image 3 Anti Rat CD45RC Antibody, clone OX-22 thumbnail image 4
Anti Rat CD45RC Antibody, clone OX-22 gallery image 1
Figure A. FITC conjugated mouse anti rat CD45RC (MCA53FT) and RPE conjugated mouse IgG1 isotype control (MCA1209PE). Figure B. FITC conjugated mouse anti rat CD45RC (MCA53FT) and RPE conjugated mouse anti rat RT1B (MCA46PE). All experiments performed on red cell lysed rat blood gated on mononuclear cells.
Anti Rat CD45RC Antibody, clone OX-22 gallery image 2
Figure A. RPE conjugated mouse anti rat RT1B (MCA46PE) and FITC conjugated mouse IgG1 isotype control (MCA1209F). Figure B. RPE conjugated mouse anti rat RT1B (MCA46PE) and FITC conjugated mouse anti rat CD45RC (MCA53FT). All experiments performed on red cell lysed rat blood gated on mononuclear cells.
Anti Rat CD45RC Antibody, clone OX-22 gallery image 3
Published customer image:
Mouse anti Rat CD45RC antibody, clone OX-22 (MCA53GA) used to identify B cells in rat sciatic nerves by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Oridonin treatment attenuated local demyelination, inflammatory cell infiltration and inflammatory cytokines in sciatic nerves of EAN rats. Following preventive or therapeutic treatments, rats were sacrificed on Day 15 and sciatic nerves were taken for LFB and immunohistochemical staining or semi-quantitative PCR. Representative microphotos of LFB and IHC staining for control (A–D) or Oridonin treated (E–H) EAN rats are shown. Quantification of demyelination, mean histological scores and percentages of immune cells marker IR on cross sections were calculated as described in Materials and Methods. Preventive or therapeutic Oridonin treatments significantly reduced the demyelination levels; mean histological scores; percentages of ED1 (macrophages), W3/13 (pan-T cells) and OX22 (B cells) IR in sciatic nerves, compared to the control EAN rats (I–K). (L) mRNA levels of IL-1β, IL-6, iNOS and IL-10 in sciatic nerves of these EAN rats was analyzed by real-time PCR. Therapeutic Oridonin treatment significantly reduced mRNA levels of IL-1β, IL-6 and iNOS in sciatic nerves, but did not change the mRNA level of IL-10 significantly. ∗p <0.05, ∗∗p <0.01 compared to the control group, n = 6.

From: Xu L, Li L, Zhang CY, Schluesener H, Zhang ZY.
Natural Diterpenoid Oridonin Ameliorates Experimental Autoimmune Neuritis by Promoting Anti-inflammatory Macrophages Through Blocking Notch Pathway.
Front Neurosci. 2019 Apr 2;13:272.
doi: 10.3389/fnins.2019.00272.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Rat CD45RC Antibody, clone OX-22 gallery image 4
Published customer image:
Mouse anti Rat CD45RC antibody, clone OX-22 (MCA53GA) used to identify B cells in rat spinal cord sections by immunofluorescence.
Image caption:
Imatinib reduces infiltration of immune cells and attenuates microglia activation.
(I–Z) IF performed on spinal cord cross-sections of rats injected with fluorescent tracer (dextran, red) on day 14 p.i. α-Ox-42, ED1, Ox-6, Ox-22, CD45RA and W3/13 antibody staining (all in green) in imatinib- (I, L, O, R, U, X) and PBS-treated rats (J, M, P, S, V, Y). (I–K) Microglia activation was significantly decreased in the imatinib-treated rats, while Ox-42+ cells were detectable around leaky blood vessels (asterix) in the control tissue. (L–N) The amount of macrophages/activated microglia cells was significantly decreased in the spinal cords of the imatinib-treated rats. (O–Q) Significantly lower amounts of MHC class II+ cells were found in the meninges and parenchyma of the imatinib-treated rats vs. PBS controls. (R–Z) Significantly lower amounts of Ox-22+, CD45RA+ and W3/13+ cells were found in the meninges and parenchyma of the imatinib-treated rats vs. PBS controls (R, U, X vs. S, V, Y). Quantifications of Ox-42, ED1, Ox-6, Ox-22, CD45RA and W3/13 expression based on green fluorescent pixel area quantifications from spinal cord cross-sections (K, N, Q, T, W, Z). n = 5 rats/experimental group. Scale bar, 50 μm. Error bars, S.E.M. Statistics were calculated using t-test and P values <0.05 were considered significant (P<0.05 = *, P<0.01 = **, P<0.001 = ***). Imatinib or PBS oral gavage was performed from day 5 p.i until the end of the experiment.

From:Adzemovic MV, Zeitelhofer M, Eriksson U, Olsson T, Nilsson I.
Imatinib ameliorates neuroinflammation in a rat model of multiple sclerosis by enhancing blood-brain barrier integrity and by modulating the peripheral immune response.
PLoS One. 2013;8(2):e56586.
doi: 10.1371/journal.pone.0056586.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Rat CD45RC:FITC

Product Type
Monoclonal Antibody
Clone
OX-22
Isotype
IgG1
Specificity
CD45RC

Quick Links:

 Antibody Quality and Performance Guarantee
 Unrivaled Technical Support
Product Code Applications Pack Size List Price Your Price Qty
MCA53FT
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Rat CD45RC antibody, clone OX-22 recognizes rat CD45RC, the high molecular weight form of the leucocyte common antigen. The antigen is found on B cells, approximately 50% of bone marrow cells, all CD8+ve T cells, but splits CD4+ve T cells into two populations, CD4+CD45RChigh (Th1-like) and CD4+CD45RClow (Th2-like).

This product is routinely tested in flow cytometry on rat splenocytes.

Target Species
Rat
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Phytohaemagglutinin (PHA) -activated rat lymphocytes
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry NEAT 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA1209F F 0.1 mg loader
>
List Price Your Price
loader
Description Mouse IgG1 Negative Control:FITC

Source Reference

  1. Spickett, G.P. et al. (1983) MRC OX-22, a monoclonal antibody that labels a new subset of T lymphocytes and reacts with the high molecular weight form of the leukocyte-common antigen.
    J Exp Med. 158 (3): 795-810.

References for CD45RC antibody

  1. Arthur, R.P. & Mason, D. (1986) T cells that help B cell responses to soluble antigen are distinguishable from those producing interleukin 2 on mitogenic or allogeneic stimulation.
    J Exp Med. 163 (4): 774-86.
  2. Pelegrí, C. et al. (2001) Prevention of adjuvant arthritis by the W3/25 anti-CD4 monoclonal antibody is associated with a decrease of blood CD4(+)CD45RC(high) T cells.
    Clin Exp Immunol. 125 (3): 470-7.
  3. Mueller, C.A. et al. (2003) Spinal cord injury induces lesional expression of the proinflammatory and antiangiogenic cytokine EMAP II.
    J Neurotrauma. 20 (10): 1007-15.
  4. Fulgenzi, A. et al. (2004) Distribution of 99mTc-labeled lymphocytes in control and inflamed rats.
    Nucl Med Biol. 31 (5): 631-8.
  5. Schwab, J.M. et al. (2005) Spinal cord injury induces early and persistent lesional P2X4 receptor expression.
    J Neuroimmunol. 163 (1-2): 185-9.
  6. Schwab, J.M. et al. (2005) Spinal cord injury-induced lesional expression of the repulsive guidance molecule (RGM).
    Eur J Neurosci. 21 (6): 1569-76.
  7. Conrad, S. et al. (2005) Prolonged lesional expression of RhoA and RhoB following spinal cord injury.
    J Comp Neurol. 487 (2): 166-75.
  8. Herrero-Fresneda, I. et al. (2005) Reduction of postischemic immune inflammatory response: an effective strategy for attenuating chronic allograft nephropathy.
    Transplantation. 79 (2): 165-73.
    9. View The Latest Product References
  9. Mueller, C.A. et al. (2007) Lesional expression of the endogenous angiogenesis inhibitor endostatin/collagen XVIII following traumatic brain injury (TBI).
    Exp Neurol. 208 (2): 228-37.
  10. Adzemovic, M.V. et al. (2013) Imatinib ameliorates neuroinflammation in a rat model of multiple sclerosis by enhancing blood-brain barrier integrity and by modulating the peripheral immune response.
    PLoS One. 8 (2): e56586.
  11. Xu, L. et al. (2019) Natural Diterpenoid Oridonin Ameliorates Experimental Autoimmune Neuritis by Promoting Anti-inflammatory Macrophages Through Blocking Notch Pathway.
    Front Neurosci. 13: 272.

RRID
AB_321431
UniProt
P04157
Entrez Gene
Ptprc
GO Terms
GO:0001915 negative regulation of T cell mediated cytotoxicity
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
GO:0005887 integral to plasma membrane
GO:0005925 focal adhesion
GO:0006469 negative regulation of protein kinase activity
GO:0006470 protein dephosphorylation
GO:0019887 protein kinase regulator activity
GO:0009898 internal side of plasma membrane
GO:0010332 response to gamma radiation
GO:0019901 protein kinase binding
GO:0042100 B cell proliferation
GO:0045121 membrane raft
GO:0050852 T cell receptor signaling pathway
GO:0050853 B cell receptor signaling pathway
GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051607 defense response to virus

MCA53FT

1014 165932

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with CD45RC specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Rat Products
Please note