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CD45R antibody | HIS24 (6B6)

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Mouse anti Rat CD45R

Product Type
Monoclonal Antibody
Clone
HIS24 (6B6)
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA1326
Datasheet
C* F
SDS
2 ml loader

Mouse anti Rat CD45R antibody, clone HIS24 recognizes the high molecular weight form of the leucocyte common antigen (LCA) or CD45. CD45R is expressed on mature and precursor B lymphocytes, and to some extent on marginal zone B cells and a small subset of T cells.

Mouse anti Rat CD45R antibody, clone HIS24 is routinely used as a pan-B-cell marker for immunohistochemical studies on brain tissue (Ali et al. 2005), pancreas (Homo-Delarche et al. 2006), pulmonary tissue (Foxwell et al. 2001) and in flow cytometry on bone marrow cells (Prakapas et al. 1993).

Product Details

Target Species
Rat
Product Form
Tissue Culture Supernatant - liquid
Preparation
Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Single cell suspension of rat peyers patches.
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse X63/Ag8 myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
P04157
Entrez Gene
Ptprc
GO Terms
GO:0001915 negative regulation of T cell mediated cytotoxicity
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
GO:0005887 integral to plasma membrane
GO:0005925 focal adhesion
GO:0006469 negative regulation of protein kinase activity
GO:0006470 protein dephosphorylation
GO:0019887 protein kinase regulator activity
GO:0009898 internal side of plasma membrane
GO:0010332 response to gamma radiation
GO:0019901 protein kinase binding
GO:0042100 B cell proliferation
GO:0045121 membrane raft
GO:0050852 T cell receptor signaling pathway
GO:0050853 B cell receptor signaling pathway
GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051607 defense response to virus
Regulatory
For research purposes only

Applications of CD45R antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 / 5
Immunohistology - Frozen 1 1 / 40
Immunohistology - Paraffin
Immunohistology - Resin
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2b Negative Control MCA691 F 100 Tests loader

Application Based External Images

Immunohistology - Frozen

Product Specific References

References for CD45R antibody

  1. Kroese, F.G. et al. (1987) B lymphocyte differentiation in the rat: production and characterization of monoclonal antibodies to B lineage-associated antigens.
    Eur J Immunol. 1987 Jul;17(7):921-8.
  2. Ali, S. et al. (2005) Combined immunostimulation and conditional cytotoxic gene therapy provide long-term survival in a large glioma model.
    Cancer Res. 65: 7194-204.
  3. Homo-Delarche, F. et al. (2006) Islet inflammation and fibrosis in a spontaneous model of type 2 diabetes, the GK rat.
    Diabetes. 2006 Jun;55(6):1625-33.
  4. Ma, H. et al. (2007) Features of chronic allograft rejection on rat small intestine transplantation. Pediatr Transplant.
    Pediatr Transplantation 11:165-72.
  5. Prakapas, Z. et al. (1993) Enrichment and characterization of thymus-repopulating cells in stroma-dependent cultures of rat bone marrow.
    J Cell Sci. 104: 1039-48.
  6. Erben, R.G. et al. (1998) Ovariectomy augments B lymphopoiesis and generation of monocyte-macrophage precursors in rat bone marrow.
    Am J Physiol. 274: E476-83.
  7. Horibe, E.K. et al. (2008) Rapamycin-conditioned, alloantigen-pulsed dendritic cells promote indefinite survival of vascularized skin allografts in association with T regulatory cell expansion.
    Transpl Immunol. 18: 307-18.
  8. Ménoret, S. et al. (2013) Generation of Rag1-knockout immunodeficient rats and mice using engineered meganucleases.
    FASEB J. 27: 703-11.
  9. Saiki, T. et al. (2001) In vivo roles of donor and host dendritic cells in allogeneic immune response: cluster formation with host proliferating T cells.
    J Leukoc Biol. 69: 705-12.
  10. Foxwell, A.R. et al. (2001) CD8+ T cells have an essential role in pulmonary clearance of nontypeable Haemophilus influenzae following mucosal immunization.
    Infect Immun. 69: 2636-42.
  11. Shen, J. et al. (1998) Histological characterization and pharmacological control of chronic rejection in xenogeneic and allogeneic heart transplantation.
    Transplantation. 1998 Sep 27;66(6):692-8.

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