Mouse anti Rat CD45R antibody, clone HIS24
recognizes the high molecular weight form of the leucocyte common antigen (LCA) or CD45. CD45R is expressed on mature and precursor B lymphocytes, and to some extent on marginal zone B cells and a small subset of T cells.
Mouse anti Rat CD45R antibody, clone HIS24 is routinely used as a pan-B-cell marker for immunohistochemical studies on brain tissue (Ali et al. 2005
), pancreas (Homo-Delarche et al. 2006
), pulmonary tissue (Foxwell et al. 2001
) and in flow cytometry on bone marrow cells (Prakapas et al. 1993
- Target Species
- Product Form
- Tissue Culture Supernatant - liquid
- Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Single cell suspension of rat peyers patches.
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse X63/Ag8 myeloma cell line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
negative regulation of T cell mediated cytotoxicity
negative regulation of cytokine-mediated signaling pathway
transmembrane receptor protein tyrosine phosphatase activity
integral to plasma membrane
negative regulation of protein kinase activity
protein kinase regulator activity
internal side of plasma membrane
response to gamma radiation
protein kinase binding
B cell proliferation
T cell receptor signaling pathway
B cell receptor signaling pathway
positive regulation of antigen receptor-mediated signaling pathway
release of sequestered calcium ion into cytosol
defense response to virus
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD45R antibody
||1 / 5
|Immunohistology - Frozen 1
||1 / 40
|Immunohistology - Paraffin
|Immunohistology - Resin
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Immunohistology - Frozen
Product Specific References
References for CD45R antibody
Kroese, F.G. et al. (1987) B lymphocyte differentiation in the rat: production and characterization of monoclonal antibodies to B lineage-associated antigens.
Eur J Immunol. 1987 Jul;17(7):921-8.
Ali, S. et al. (2005) Combined immunostimulation and conditional cytotoxic gene therapy provide long-term survival in a large glioma model.
Cancer Res. 65: 7194-204.
Homo-Delarche, F. et al. (2006) Islet inflammation and fibrosis in a spontaneous model of type 2 diabetes, the GK rat.
Diabetes. 2006 Jun;55(6):1625-33.
Ma, H. et al. (2007) Features of chronic allograft rejection on rat small intestine transplantation. Pediatr Transplant.
Pediatr Transplantation 11:165-72.
Prakapas, Z. et al. (1993) Enrichment and characterization of thymus-repopulating cells in stroma-dependent cultures of rat bone marrow.
J Cell Sci. 104: 1039-48.
Erben, R.G. et al. (1998) Ovariectomy augments B lymphopoiesis and generation of monocyte-macrophage precursors in rat bone marrow.
Am J Physiol. 274: E476-83.
Horibe, E.K. et al. (2008) Rapamycin-conditioned, alloantigen-pulsed dendritic cells promote indefinite survival of vascularized skin allografts in association with T regulatory cell expansion.
Transpl Immunol. 18: 307-18.
Ménoret, S. et al. (2013) Generation of Rag1-knockout immunodeficient rats and mice using engineered meganucleases.
FASEB J. 27: 703-11.
Saiki, T. et al. (2001) In vivo roles of donor and host dendritic cells in allogeneic immune response: cluster formation with host proliferating T cells.
J Leukoc Biol. 69: 705-12.
Foxwell, A.R. et al. (2001) CD8+ T cells have an essential role in pulmonary clearance of nontypeable Haemophilus influenzae following mucosal immunization.
Infect Immun. 69: 2636-42.
Shen, J. et al. (1998) Histological characterization and pharmacological control of chronic rejection in xenogeneic and allogeneic heart transplantation.
Transplantation. 1998 Sep 27;66(6):692-8.
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