CD45R antibody | HIS24 (6B6)

Mouse anti Rat CD45R antibody, clone HIS24 is routinely used as a pan-B-cell marker for immunohistochemical studies on brain tissue (Ali et al. 2005), pancreas (Homo-Delarche et al. 2006), pulmonary tissue (Foxwell et al. 2001) and in flow cytometry on bone marrow cells (Prakapas et al. 1993).
Product Details
- Target Species
- Rat
- Product Form
- Tissue Culture Supernatant - liquid
- Preparation
- Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Single cell suspension of rat peyers patches.
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse X63/Ag8 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
More Information
- UniProt
- P04157 Related reagents
- Entrez Gene
- Ptprc Related reagents
- GO Terms
- GO:0001915 negative regulation of T cell mediated cytotoxicity
- GO:0001960 negative regulation of cytokine-mediated signaling pathway
- GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
- GO:0005887 integral to plasma membrane
- GO:0005925 focal adhesion
- GO:0006469 negative regulation of protein kinase activity
- GO:0006470 protein dephosphorylation
- GO:0019887 protein kinase regulator activity
- GO:0009898 internal side of plasma membrane
- GO:0010332 response to gamma radiation
- GO:0019901 protein kinase binding
- GO:0042100 B cell proliferation
- GO:0045121 membrane raft
- GO:0050852 T cell receptor signaling pathway
- GO:0050853 B cell receptor signaling pathway
- GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
- GO:0051209 release of sequestered calcium ion into cytosol
- GO:0051607 defense response to virus
- Regulatory
- For research purposes only
Applications of CD45R antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1 / 5 | ||
Immunohistology - Frozen 1 | 1 / 40 | ||
Immunohistology - Paraffin | |||
Immunohistology - Resin |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG2b Negative Control | MCA691 | F | 100 Tests |
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Product Specific References
References for CD45R antibody
-
Kroese, F.G. et al. (1987) B lymphocyte differentiation in the rat: production and characterization of monoclonal antibodies to B lineage-associated antigens.
Eur J Immunol. 1987 Jul;17(7):921-8. -
Ali, S. et al. (2005) Combined immunostimulation and conditional cytotoxic gene therapy provide long-term survival in a large glioma model.
Cancer Res. 65: 7194-204. -
Homo-Delarche, F. et al. (2006) Islet inflammation and fibrosis in a spontaneous model of type 2 diabetes, the GK rat.
Diabetes. 2006 Jun;55(6):1625-33. -
Ma, H. et al. (2007) Features of chronic allograft rejection on rat small intestine transplantation. Pediatr Transplant.
Pediatr Transplantation 11:165-72. -
Prakapas, Z. et al. (1993) Enrichment and characterization of thymus-repopulating cells in stroma-dependent cultures of rat bone marrow.
J Cell Sci. 104: 1039-48. -
Erben, R.G. et al. (1998) Ovariectomy augments B lymphopoiesis and generation of monocyte-macrophage precursors in rat bone marrow.
Am J Physiol. 274: E476-83. -
Horibe, E.K. et al. (2008) Rapamycin-conditioned, alloantigen-pulsed dendritic cells promote indefinite survival of vascularized skin allografts in association with T regulatory cell expansion.
Transpl Immunol. 18: 307-18. -
Ménoret, S. et al. (2013) Generation of Rag1-knockout immunodeficient rats and mice using engineered meganucleases.
FASEB J. 27: 703-11. -
Saiki, T. et al. (2001) In vivo roles of donor and host dendritic cells in allogeneic immune response: cluster formation with host proliferating T cells.
J Leukoc Biol. 69: 705-12. -
Foxwell, A.R. et al. (2001) CD8+ T cells have an essential role in pulmonary clearance of nontypeable Haemophilus influenzae following mucosal immunization.
Infect Immun. 69: 2636-42. -
Shen, J. et al. (1998) Histological characterization and pharmacological control of chronic rejection in xenogeneic and allogeneic heart transplantation.
Transplantation. 1998 Sep 27;66(6):692-8.