CD45 antibody | OX-29

Mouse anti Rat CD45

Product Type
Monoclonal Antibody
Clone
OX-29
Isotype
IgG2a
Specificity
CD45

Product Code Applications Pack Size List Price Your Price Qty
MCA589R
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP 0.25 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Rat CD45 antibody, clone OX-29 recognizes rat CD45, also known as Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (LCA) or T200, expressed on all rat leukocytes. CD45 is a single pass, type I transmembrane glycoprotein of ~180-240kDa depending on the isoform and degree of glycosylation. Rat CD45 contains two intracellular tyrosine-protein phosphatase domains and at least two extracellular fibronectin type-III domains (Uniprot Acc no: P04157), although the existence of a third similar domain has previously been proposed (Symons et al. 1999).

Mouse anti Rat CD45 antibody, clone OX-29 has been reported as recognizing a monomorphic determinant present on all allogenic forms of the rat CD45 antigen (Symons and Barclay 2000). Epitope mapping further indicates that the OX-29 clone binds to a determinant located within the membrane distal two domains of CD45 (Symons, et al. 1999).

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
L-CA (CD45)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Fusion of cells from NSO mouse myeloma line with spleen cells from BALB/c mice immunized with purified LCA.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/50
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD45 antibody

  1. Woollett, G.R. et al. (1985) Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes.
    Eur J Immunol. 15 (2): 168-73.
  2. Barclay, A.N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
    Immunology. 42 (4): 593-600.
  3. Symons, A. et al. (1999) Domain organization of the extracellular region of CD45.
    Protein Eng. 12 (10): 885-92.
  4. Zhou, L. et al. (2014) Purification of endothelial cells from rodent brain by immunopanning.
    Cold Spring Harb Protoc. 2014 (1): 65-77.
  5. Barres, B.A. (2014) Designing and troubleshooting immunopanning protocols for purifying neural cells.
    Cold Spring Harb Protoc. 2014 (12): 1342-7.

Synonyms
LCA
RRID
AB_931751
UniProt
P04157
Entrez Gene
Ptprc
GO Terms
GO:0001915 negative regulation of T cell mediated cytotoxicity
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
GO:0005887 integral to plasma membrane
GO:0005925 focal adhesion
GO:0006469 negative regulation of protein kinase activity
GO:0006470 protein dephosphorylation
GO:0019887 protein kinase regulator activity
GO:0009898 internal side of plasma membrane
GO:0010332 response to gamma radiation
GO:0019901 protein kinase binding
GO:0042100 B cell proliferation
GO:0045121 membrane raft
GO:0050852 T cell receptor signaling pathway
GO:0050853 B cell receptor signaling pathway
GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051607 defense response to virus
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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