Mouse anti Rat CD45 antibody, clone OX-29
recognizes rat CD45, also known as Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (LCA) or T200, expressed on all rat leukocytes. CD45 is a single pass, type I transmembrane glycoprotein of ~180-240kDa depending on the isoform and degree of glycosylation. Rat CD45 contains two intracellular tyrosine-protein phosphatase domains
and at least two extracellular fibronectin type-III domains
(Uniprot Acc no: P04157
), although the existence of a third similar domain has previously been proposed (Symons et al. 1999
Mouse anti Rat CD45 antibody, clone OX-29 has been reported as recognizing a monomorphic determinant present on all allogenic forms of the rat CD45 antigen (Symons and Barclay 2000
). Epitope mapping further indicates that the OX-29 clone binds to a determinant located within the membrane distal two domains of CD45 (Symons, et al. 1999
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- L-CA (CD45)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Fusion of cells from NSO mouse myeloma line with spleen cells from BALB/c mice immunised with purified LCA.
- Store at +4oC for one month or at -20oC for longer.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
negative regulation of T cell mediated cytotoxicity
negative regulation of cytokine-mediated signaling pathway
transmembrane receptor protein tyrosine phosphatase activity
integral to plasma membrane
negative regulation of protein kinase activity
protein kinase regulator activity
internal side of plasma membrane
response to gamma radiation
protein kinase binding
B cell proliferation
T cell receptor signaling pathway
B cell receptor signaling pathway
positive regulation of antigen receptor-mediated signaling pathway
release of sequestered calcium ion into cytosol
defense response to virus
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD45 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available