Browsing Modes for Business Users

CD45 antibody | OX-1

Mouse anti Rat CD45:Alexa Fluor® 647

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price

Mouse anti Rat CD45 antibody, clone OX-1 recognizes CD45, also known as the leucocyte common antigen (LCA). The leucocyte common antigen consists of a family of heavily glycosylated membrane glycoproteins of molecular weight 180 – 240kDa.

Antibodies recognising a common epitope on all of these isoforms are termed CD45, whilst those recognising only individual isoforms are termed CD45RA, CD45RO etc. OX-1 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes.

CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.

This product is routinely tested in flow cytometry on rat splenocytes

Target Species
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Rat thymocyte membrane glycoproteins.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
For research purposes only
12 months from date of despatch
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA1209A647 F 100 Tests/1ml loader
List Price Your Price
Description Mouse IgG1 Negative Control:Alexa Fluor® 647

References for CD45 antibody

  1. Standring, R. et al. (1978) The predominant heavily glycosylated glycoproteins at the surface of rat lymphoid cells are differentiation antigens.
    Eur J Immunol. 8 (12): 832-9.
  2. Sunderland, C.A. et al. (1979) Purification with monoclonal antibody of a predominant leukocyte-common antigen and glycoprotein from rat thymocytes.
    Eur J Immunol. 9 (2): 155-9.
  3. Woollett, G.R. et al. (1985) Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes.
    Eur J Immunol. 15 (2): 168-73.
  4. Martín, A. et al. (1995) Passive dual immunization against tumour necrosis factor-alpha (TNF-alpha) and IL-1 beta maximally ameliorates acute aminonucleoside nephrosis.
    Clin Exp Immunol. 99 (2): 283-8.
  5. Giezeman-Smits, K.M. et al. (1999) The regulatory role of CD45 on rat NK cells in target cell lysis.
    J Immunol. 163 (1): 71-6.
  6. Murakami, K. et al. (2000) Regulation of mast cell signaling through high-affinity IgE receptor by CD45 protein tyrosine phosphatase.
    Int Immunol. 12 (2): 169-76.
  7. Ermert, L. et al. (2001) Comparison of different detection methods in quantitative microdensitometry.
    Am J Pathol. 158: 407-17.
  8. Dick, A.D. et al. (2001) Distribution of OX2 antigen and OX2 receptor within retina.
    Invest Ophthalmol Vis Sci. 42: 170-6.
  9. View The Latest Product References
  10. Sato, K. et al. (2001) Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat cardiac transplants.
    J Immunol. 166 (6): 4185-94.
  11. Kurozumi, K. et al. (2007) Effect of tumor microenvironment modulation on the efficacy of oncolytic virus therapy.
    J Natl Cancer Inst. 99: 1768-81.
  12. Leonardo, C.C. et al. (2009) Inhibition of gelatinase activity reduces neural injury in an ex vivo model of hypoxia-ischemia.
    Neuroscience. 160: 755-66.
  13. Vaschetto, R. et al. (2010) Renal hypoperfusion and impaired endothelium-dependent vasodilation in an animal model of VILI: the role of the peroxynitrite-PARP pathway
    Crit Care. 14: R45.
  14. Ladhoff, J. et al. (2010) Immune privilege of endothelial cells differentiated from endothelial progenitor cells.
    Cardiovasc Res. 88: 121-9.
  15. Jeong, H.K. et al (2010) Inflammatory responses are not sufficient to cause delayed neuronal death in ATP-induced acute brain injury.
    PLoS One. 5: e13756.
  16. Schupp, N. et al. (2011) Mineralocorticoid receptor-mediated DNA damage in kidneys of DOCA-salt hypertensive rats.
    FASEB J. 25 (3): 968-78.
  17. Markusic, D.M. et al. (2010) Separating lentiviral vector injection and induction of gene expression in time, does not prevent an immune response to rtTA in rats.
    PLoS One. 5: e9974.
  18. Runesson, E. et al. (2015) Nucleostemin- and Oct 3/4-positive stem/progenitor cells exhibit disparate anatomical and temporal expression during rat Achilles tendon healing.
    BMC Musculoskelet Disord. 16: 212.
  19. Tanner, D.C. et al. (2015) cFLIP is critical for oligodendrocyte protection from inflammation.
    Cell Death Differ. 22 (9): 1489-501.
  20. Wang, C. et al. (2015) Small activating RNA induces myogenic differentiation of rat adipose-derived stem cells by upregulating MyoD.
    Int Braz J Urol. 41 (4): 764-72.
  21. Yao, Y. et al. (2016) Alendronate Attenuates Spinal Microglial Activation and Neuropathic Pain.
    J Pain. 17 (8): 889-903.
  22. Collins, J.J.P. et al. (2018) Impaired Angiogenic Supportive Capacity and Altered Gene Expression Profile of Resident CD146+ Mesenchymal Stromal Cells Isolated from Hyperoxia-Injured Neonatal Rat Lungs.
    Stem Cells Dev. 27 (16): 1109-24.
  23. Porwal, K. et al. (2019) Increased bone marrow-specific adipogenesis by clofazimine causes impaired fracture healing, osteopenia and osteonecrosis without extra-skeletal effects in rats.
    Toxicol Sci. kfz172.
  24. Hellenbrand, D.J. et al. (2019) Sustained interleukin-10 delivery reduces inflammation and improves motor function after spinal cord injury.
    J Neuroinflammation. 16 (1): 93.
  25. Kuriyama, T. et al. (2020) A novel rat model of inflammatory bowel disease developed using a device created with a 3D printer.
    Regen Ther. 14: 1-10.
  26. Pilipović, I. et al. (2020) Propranolol diminished severity of rat EAE by enhancing immunoregulatory/protective properties of spinal cord microglia.
    Neurobiol Dis. 134: 104665.
  27. Dabrowska, S. et al. (2021) Neuroinflammation evoked by brain injury in a rat model of lacunar infarct.
    Exp Neurol. 336: 113531.
  28. Elabi, O.F. et al. (2021) L-dopa-Dependent Effects of GLP-1R Agonists on the Survival of Dopaminergic Cells Transplanted into a Rat Model of Parkinson Disease.
    Int J Mol Sci. 22(22):12346.
  29. Hou, Y. et al. (2021) Pseudoginsenoside-F11 promotes functional recovery after transient cerebral ischemia by regulating the microglia/macrophage polarization in rats.
    Int Immunopharmacol. 99: 107896.
  30. Eweida, A. et al. (2022) Systemically injected bone marrow mononuclear cells specifically home to axially vascularized tissue engineering constructs.
    PLoS One. 17 (8): e0272697.
  31. Yang, Q. et al. (2022) Electrospun aligned poly(ε-caprolactone) nanofiber yarns guiding 3D organization of tendon stem/progenitor cells in tenogenic differentiation and tendon repair.
    Front Bioeng Biotechnol. 10: 960694.
  32. Wu, Y. et al. (2018) Increased ceruloplasmin expression caused by infiltrated leukocytes, activated microglia, and astrocytes in injured female rat spinal cords.
    J Neurosci Res. 96 (7): 1265-76.


Immunohistology - Frozen

Entrez Gene
GO Terms
GO:0001915 negative regulation of T cell mediated cytotoxicity
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
GO:0005887 integral to plasma membrane
GO:0005925 focal adhesion
GO:0006469 negative regulation of protein kinase activity
GO:0006470 protein dephosphorylation
GO:0019887 protein kinase regulator activity
GO:0009898 internal side of plasma membrane
GO:0010332 response to gamma radiation
GO:0019901 protein kinase binding
GO:0042100 B cell proliferation
GO:0045121 membrane raft
GO:0050852 T cell receptor signaling pathway
GO:0050853 B cell receptor signaling pathway
GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051607 defense response to virus


150533 167532

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with CD45 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Rat Products
Please note