Mouse anti Rat CD4:FITC/CD25:RPE dual color reagent is a dual colour reagent designed to allow the simultaneous enumeration of cells expressing the rat CD4 and CD25 cell surface antigens.
Clone W3/25 recognises rat CD4, a T cell differentiation antigen expressed by helper T lymphocytes and weakly by monocytes. Clone OX-39 recognises rat CD25, a ~55 kDa glycoprotein expressed by activated T lymphocytes.
- Target Species
- Product Form
- Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
- Reconstitute with 1 ml distilled water
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
|1%||Bovine Serum Albumin|
- Antibody Isotypes
- FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG1 (MOUSE)
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of reconstitution.
- Entrez Gene
- GO Terms
integral to membrane
protein kinase binding
T cell selection
inflammatory response to antigenic stimulus
interleukin-2 receptor activity
positive regulation of T cell differentiation
negative regulation of defense response to virus
negative regulation of immune response
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cell in 100ul.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Negative Isotype Controls Available
Product Specific References
References for CD4/CD25
Barclay, A. N. et al. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
Immunol. 42: 593-600.
Paterson, D.J. et al. (1987) Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts.
Mol Immunol. 24 (12): 1281-90.
Zschemisch, N.H. et al. (2012) Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat.
BMC Immunol. 13: 60.