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CD4/CD25 | W3/25 / OX-39

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Mouse anti Rat CD4:FITC/CD25:RPE

Product Type
Dual Color Reagent
Clone
W3/25 / OX-39
Isotype
Cocktail
Specificity
CD4/CD25

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Product Code Applications Pack Size List Price Your Price Qty
DC040
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests
List Price Your Price

Mouse anti Rat CD4:FITC/CD25:RPE dual color reagent is a dual colour reagent designed to allow the simultaneous enumeration of cells expressing the rat CD4 and CD25 cell surface antigens.

Clone W3/25 recognises rat CD4, a T cell differentiation antigen expressed by helper T lymphocytes and weakly by monocytes. Clone OX-39 recognises rat CD25, a ~55 kDa glycoprotein expressed by activated T lymphocytes.

Target Species
Rat
Product Form
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Antibody Isotypes
FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG1 (MOUSE)
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
RPE 488nm laser 496 578
RPE 561nm laser 546 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cell in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1:FITC/Mouse IgG1:RPE Negative Control DC045 F 100 Tests
List Price Your Price
Description Mouse IgG1:FITC/Mouse IgG1:RPE Negative Control

References for CD4/CD25

  1. Barclay, A. N. et al. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
    Immunol. 42: 593-600.
  2. Paterson, D.J. et al. (1987) Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts.
    Mol Immunol. 24 (12): 1281-90.
  3. Zschemisch, N.H. et al. (2012) Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat.
    BMC Immunol. 13: 60.

RRID
AB_324136
UniProt
P05540
P26897
Entrez Gene
Cd4
Il2ra
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0006955 immune response
GO:0019901 protein kinase binding
GO:0045058 T cell selection
GO:0002437 inflammatory response to antigenic stimulus
GO:0004911 interleukin-2 receptor activity
GO:0008144 drug binding
GO:0045582 positive regulation of T cell differentiation
GO:0050687 negative regulation of defense response to virus
GO:0050777 negative regulation of immune response

DC040

155113

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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