CD4 antibody | W3/25
Dynamics of Th1 cell population. E–G) Photographs of double immunostained sections showing a representative CD4+Tbet+ cell found around blood vessels (BV) (arrows point to double-immunolabelled cell). Bar scale = 20 μm.
From: Almolda B, Costa M, Montoya M, González B, Castellano B (2011)
Increase in Th17 and T-reg Lymphocytes and Decrease of IL22 Correlate with the Recovery Phase of Acute EAE IN Rat.
PLoS ONE 6(11): e27473.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Image caption:
Dynamics of Th17 cells. D–F) Photographs of double immunolabelled sections showing a representative CD4+RORγ+ cell (arrow) observed around blood vessels (BV). Bar scale = 30 μm.
Almolda B, Costa M, Montoya M, González B, Castellano B (2011) Increase in Th17 and T-reg Lymphocytes and Decrease of IL22 Correlate with the Recovery Phase of Acute EAE IN Rat. PLoS ONE 6(11): e27473.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Image caption:
Dynamics of T-regulatory cells. D–F) Photographs of double immunolabelled sections showing a representative CD4+Foxp3+ cell (arrows) found around blood vessels (BV). Bar scale = 30 μm.
From: Almolda B, Costa M, Montoya M, González B, Castellano B (2011)
Increase in Th17 and T-reg Lymphocytes and Decrease of IL22 Correlate with the Recovery Phase of Acute EAE IN Rat.
PLoS ONE 6(11): e27473.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Image caption:
Apoptotic lymphocytes. A–C) Double immunolabelling combining the microglial marker Iba1 and CD4, and counterstained with DAPI. Iba1+ microglial cells (green) were observed closely related to apoptotic cells that were identified as CD4+ lymphocytes (arrows in A, B and C). Bar scale = 20 μm
From: Almolda B, Costa M, Montoya M, González B, Castellano B (2011)
Increase in Th17 and T-reg Lymphocytes and Decrease of IL22 Correlate with the Recovery Phase of Acute EAE IN Rat.
PLoS ONE 6(11): e27473.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Image caption:
Photographs showing the CD4 (A) and Tbet (B) immunostatining at score 0R. Note that the few Tbet+ cells did not colocalize with CD4 (arrows in A–C). D–F) Colocalization between CD4 and ROR-γ was found at score 0R in some cells located near blood vessels (arrows). Bar scale = 30 μm
From: Almolda B, Costa M, Montoya M, González B, Castellano B (2011)
Increase in Th17 and T-reg Lymphocytes and Decrease of IL22 Correlate with the Recovery Phase of Acute EAE IN Rat.
PLoS ONE 6(11): e27473.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Image caption:
Cells immunolabelled with CD3 and CD4 were observed at the different scores of EAE evolution (arrows). Note that, in addition to these double positive cells, also few CD3+CD4- cells (arrowheads in B and D) and CD3-CD4+ cells (asteriscs in A, B and C) were also observed. Bar scale = 30 μm
From: Almolda B, Costa M, Montoya M, González B, Castellano B (2011)
Increase in Th17 and T-reg Lymphocytes and Decrease of IL22 Correlate with the Recovery Phase of Acute EAE IN Rat.
PLoS ONE 6(11): e27473.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD4 antibody, clone W3/25 (MCA55R) used to label T helper lymphocytes in rat lymph nodes by immunohistochemistry on cryostat sections.
Image caption:
Afferent lymphatics and subcapsular sinus of the rat popliteal lymph node in initial massive S. epidermidis infection (days 1–7) with early node’s evaluation (day 8). (A) T helper lymphocytes and monocytes (CD4+, red) entering the popliteal lymph node via afferent lymphatics, magnification 600×. (B) Migrating dendritic cells (OX62+, red) in the subcapsular sinus, magnification 1000×.
From: Cąkała-Jakimowicz, M.; Puzianowska-Kuznicka, M.
Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis.
Biomedicines 2022, 10, 1021.
doi: 10.3390/biomedicines10051021
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD4 antibody, clone W3/25 (MCA55R) used to label T helper lymphocytes and monocytes in rat lymph nodes by immunohistochemistry on cryostat sections.
Image caption:
The rat popliteal lymph node with the visible follicle stained for the presence of T helper lymphocytes and monocytes (CD4+, red). (A) The node of the control animal receiving 0.9% NaCl (days 1–7, node evaluation on day 8). (B) The node after initial massive S. epidermidis infection (days 1–7) with early node evaluation (day 8). (C) The node after secondary S. epidermidis infection (day 28, node evaluation on day 29). Magnification 200×.
From: Cąkała-Jakimowicz, M.; Puzianowska-Kuznicka, M.
Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis.
Biomedicines 2022, 10, 1021.
doi: 10.3390/biomedicines10051021
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD4 antibody, clone W3/25 (MCA55R) used to label T helper lymphocytes and monocytes in rat lymph nodes by immunohistochemistry on cryostat sections.
Image caption:
The medulla of the rat popliteal lymph node in initial massive S. epidermidis infection (days 1–7) with early node’s evaluation (day 8). The node stained for: (A) Dendritic cells (OX62+, red). (B) Macrophages and monocytes (CD68+, red). (C) Stem cells, thymocytes and immature B cells (CD90+, red). (D) T helper lymphocytes and monocytes (CD4+, red). (E) Activated antigen-presenting cells (MHC class II+, red). Magnification 1000×. E.
From: Cąkała-Jakimowicz, M.; Puzianowska-Kuznicka, M.
Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis.
Biomedicines 2022, 10, 1021.
doi: 10.3390/biomedicines10051021
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD4 (Domain 1)
- Product Type
- Monoclonal Antibody
- Clone
- W3/25
- Isotype
- IgG1
- Specificity
- CD4
- Region
- (DOMAIN 1)
Antibody Quality and Performance Guarantee
| Mouse anti Rat CD4 antibody, clone W3/25 recognizes the rat CD4 cell surface glycoprotein, a ~55 kDa molecule expressed by helper T cells and weakly by monocytes. This antibody inhibits proliferation and IL-2 production in the MLR reaction. Mouse anti Rat CD4 antibody, clone W3/25 has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) (Whiteland et al. 1995). Mouse anti Rat CD4 antibody, clone W3/25 is routinely tested in flow cytometry on rat splenocytes. |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- MCA55R: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- MCA55GA, MCA55G: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Rat Thymocyte Membrane Glycoproteins.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mouse were fused with cells of the mouse NS-1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/100 | 1/200 |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin 1 | |
- 1PLP fixation is recommended for optimal results, see Whiteland et al. for details
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
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Log in | ||||||
| Description | Mouse IgG1 Negative Control | ||||||
References for CD4 antibody
-
Williams, A.F. et al. (1977) Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: differentiation antigens of rat lymphocytes.
Cell. 12 (3): 663-73. -
Barclay, A.N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
Immunology. 42 (4): 593-600. -
Pelegrí, C. et al. (1995) Immunohistochemical changes in synovial tissue during the course of adjuvant arthritis.
J Rheumatol. 22 (1): 124-32. -
Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
J Histochem Cytochem. 43 (3): 313-20. -
Bjersing, J.L. et al. (2002) Loss of ileal IgA+ plasma cells and of CD4+ lymphocytes in ileal Peyer's patches of vitamin A deficient rats.
Clin Exp Immunol. 130 (3): 404-8. -
Hofmann, N. et al. (2002) Increased expression of ICAM-1, VCAM-1, MCP-1, and MIP-1 alpha by spinal perivascular macrophages during experimental allergic encephalomyelitis in rats.
BMC Immunol. 3: 11. -
Banerjee, S. et al. (2003) Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats.
Br J Ophthalmol. 87 (12): 1515-22. -
Ishizuka, S. et al. (2004) Fermentable dietary fiber potentiates the localization of immune cells in the rat large intestinal crypts.
Exp Biol Med (Maywood). 229 (9): 876-84.
View The Latest Product References
-
Latta, M. et al. (2007) CXCR6 is expressed on T cells in both T helper type 1 (Th1) inflammation and allergen-induced Th2 lung inflammation but is only a weak mediator of chemotaxis.
Immunology. 121 (4): 555-64. -
Bode, U. et al. (2008) Dendritic cell subsets in lymph nodes are characterized by the specific draining area and influence the phenotype and fate of primed T cells.
Immunology. 123 (4): 480-90. -
Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and leukocyte infiltration in the transgenic rat model of tauopathy.
J Neuroimmunol. 209 (1-2): 16-25. -
Schwartzkopff, J. et al. (2010) NK cell depletion delays corneal allograft rejection in baby rats.
Mol Vis. 16: 1928-35. -
Schmiedl, A. et al. (2021) Lung development and immune status under chronic LPS exposure in rat pups with and without CD26/DPP4 deficiency.
Cell Tissue Res. Oct 04 [Epub ahead of print]. -
Matsuyama, S. et al. (2021) Properties of macrophages and lymphocytes appearing in rat renal fibrosis followed by repeated injection of cisplatin.
J Vet Med Sci. 83 (9): 1435-42. -
Cąkała-Jakimowicz, M. & Puzianowska-Kuznicka, M. (2022) Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis..
Biomedicines. 10 (5): 1021.
- RRID
- AB_321377
- UniProt
- P05540
- Entrez Gene
- Cd4
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0019901 protein kinase binding
- GO:0045058 T cell selection
View more products with CD4 specificity
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