CD3/CD8 | IF4 / OX-8
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DC042 is a dual colour reagent recognising rat CD3 and CD8 cell surface antigens.
Clone IF4 recognises rat CD3, a cell surface glycoprotein expressed by mature T lymphocytes. Clone OX8 recognises rat CD8, a cell surface glycoprotein expressed by thymocytes and by the suppressor/cytotoxic subset of peripheral blood T cells.
CD8 expression has also been reported upon rat macrophages.
Clone IF4 recognises rat CD3, a cell surface glycoprotein expressed by mature T lymphocytes. Clone OX8 recognises rat CD8, a cell surface glycoprotein expressed by thymocytes and by the suppressor/cytotoxic subset of peripheral blood T cells.
CD8 expression has also been reported upon rat macrophages.
Product Details
- Target Species
- Rat
- Product Form
- Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
- Reconstitution
- Reconstitute with 1 ml distilled water
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Antibody Isotypes
- FITC reagent: IgM (MOUSE)
RPE reagent: IgG1 (MOUSE)
Storage Information
- Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P19377
- Q64159
- P07725
- P05541
- Entrez Gene
- Cd8b
- Cd8a
- Cd3d
- Cd3g
- GO Terms
- GO:0016021 integral to membrane
- GO:0006954 inflammatory response
- GO:0019901 protein kinase binding
- GO:0004888 transmembrane receptor activity
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0045059 positive thymic T cell selection
- GO:0007163 establishment or maintenance of cell polarity
- GO:0015031 protein transport
- GO:0042981 regulation of apoptosis
- GO:0046982 protein heterodimerization activity
- Regulatory
- For research purposes only
Applications of CD3/CD8
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for CD3/CD8
-
Tanaka, T. et al. (1989) Characterization of a CD3-like rat T cell surface antigen recognized by a monoclonal antibody.
J Immunol. 142 (8): 2791-5. -
Brideau, R.J. et al. (1980) Two subsets of rat T lymphocytes defined with monoclonal antibodies.
Eur J Immunol. 10 (8): 609-15. -
Zschemisch, N.H. et al. (2012) Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat.
BMC Immunol. 13: 60.
Fluorescent Spectraviewer
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