CD3/CD8 | IF4/OX-8

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Mouse anti Rat CD3:FITC/CD8:RPE

Product Type
Dual Color Reagent
Clone
IF4/OX-8
Isotype
Cocktail
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
DC042 F 100 Tests
DC042 is a dual colour reagent recognising rat CD3 and CD8 cell surface antigens.

Clone IF4 recognises rat CD3, a cell surface glycoprotein expressed by mature T lymphocytes. Clone OX8 recognises rat CD8, a cell surface glycoprotein expressed by thymocytes and by the suppressor/cytotoxic subset of peripheral blood T cells.

CD8 expression has also been reported upon rat macrophages.

Product Details

Target Species
Rat
Product Form
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
Reconstitution
Reconstitute with 1 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Antibody Isotypes
FITC reagent: IgM (MOUSE)
RPE reagent: IgG1 (MOUSE)

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P19377 Related reagents
Q64159 Related reagents
P07725 Related reagents
P05541 Related reagents
Entrez Gene
Cd8b Related reagents
Cd8a Related reagents
Cd3d Related reagents
Cd3g Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0006954 inflammatory response
GO:0019901 protein kinase binding
GO:0004888 transmembrane receptor activity
GO:0007166 cell surface receptor linked signaling pathway
GO:0045059 positive thymic T cell selection
GO:0007163 establishment or maintenance of cell polarity
GO:0015031 protein transport
GO:0042981 regulation of apoptosis
GO:0046982 protein heterodimerization activity
Regulatory
For research purposes only

Applications of CD3/CD8

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Product Specific References

References for CD3/CD8

  1. Tanaka, T. et al. (1989) Characterization of a CD3-like rat T cell surface antigen recognized by a monoclonal antibody.
    J Immunol. 142 (8): 2791-5.
  2. Brideau, R.J. et al. (1980) Two subsets of rat T lymphocytes defined with monoclonal antibodies.
    Eur J Immunol. 10 (8): 609-15.
  3. Zschemisch, N.H. et al. (2012) Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat.
    BMC Immunol. 13: 60.