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CD3/CD45RA/CD161 | 1F4 / OX-33 / 10/78

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anti Rat CD3:FITC/CD45RA:RPE/CD161:Alexa Fluor® 647

Product Type
Triple Color Reagent
Clone
1F4 / OX-33 / 10/78
Isotype
Cocktail
Product Code Applications Pack Size List Price Quantity
TC019
Datasheet
F
MSDS
50 Tests loader

Anti Rat CD3:FITC/CD45RA:RPE/CD161:Alexa Fluor® 647 is a triple color reagent which can be used for the phenotyping and enumeration of rat T lymphocytes, B lymphocytes and NK cells.

Antibodies produced by clone 1F4 recognize rat CD3, a ~25 kDa cell surface antigen antigen which is expressed by mature rat T-cells.

Antibodies produced by clone OX-33 recognize rat CD45RA, a cell surface glycoprotein expressed by mature B-cells.

Antibodies produced by clone 10/78 recognize rat CD161 (NKR-PI), a ~60 kDa glycoprotein expressed on rat natural killer (NK) cells and T-cell subpopulations.

Product Details

Target Species
Rat
Product Form
Triple Color combination consisting of FITC, RPE and Alexa Fluor® 647 conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
Reconstitution
Reconstitute with 0.5 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
5%Sucrose
Antibody Isotypes
CD3 FITC reagent: Mouse IgM
CD45RA RPE reagent: Mouse IgG1
CD161 A647 reagent: Mouse IgG1

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of reconstitution

More Information

UniProt
P27471
A4KWA1
P19377
Q64159
P04157
Entrez Gene
Klrb1b
Ptprc
Cd3d
Klrb1a
Cd3g
GO Terms
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0005529 sugar binding
GO:0001915 negative regulation of T cell mediated cytotoxicity
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
GO:0005887 integral to plasma membrane
GO:0005925 focal adhesion
GO:0006469 negative regulation of protein kinase activity
GO:0006470 protein dephosphorylation
GO:0019887 protein kinase regulator activity
GO:0009898 internal side of plasma membrane
GO:0010332 response to gamma radiation
GO:0019901 protein kinase binding
GO:0042100 B cell proliferation
GO:0045121 membrane raft
GO:0050852 T cell receptor signaling pathway
GO:0050853 B cell receptor signaling pathway
GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051607 defense response to virus
GO:0004888 transmembrane receptor activity
GO:0007166 cell surface receptor linked signaling pathway
GO:0045059 positive thymic T cell selection
GO:0007163 establishment or maintenance of cell polarity
GO:0015031 protein transport
GO:0042981 regulation of apoptosis
GO:0046982 protein heterodimerization activity
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD3/CD45RA/CD161

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
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