CD3/CD45RA/CD161 | 1F4 / OX-33 / 10/78

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anti Rat CD3:FITC/CD45RA:RPE/CD161:Alexa Fluor® 647

Product Type: Triple Color Reagent
Clone: 1F4 / OX-33 / 10/78
Isotype: Cocktail

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
TC019	F			50 Tests

Summary

Anti Rat CD3:FITC/CD45RA:RPE/CD161:Alexa Fluor® 647 is a triple color reagent which can be used for the phenotyping and enumeration of rat T lymphocytes, B lymphocytes and NK cells.

Antibodies produced by clone 1F4 recognize rat CD3, a ~25 kDa cell surface antigen antigen which is expressed by mature rat T-cells.

Antibodies produced by clone OX-33 recognize rat CD45RA, a cell surface glycoprotein expressed by mature B-cells.

Antibodies produced by clone 10/78 recognize rat CD161 (NKR-PI), a ~60 kDa glycoprotein expressed on rat natural killer (NK) cells and T-cell subpopulations.

Product Details

Target Species

Rat

Product Form

Triple Color combination consisting of FITC, RPE and Alexa Fluor® 647 conjugated monoclonal antibodies mixed in optimal ratio - lyophilized

Reconstitution

Reconstitute with 0.5 ml distilled water

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide (NaN₃)
1%	Bovine Serum Albumin
5%	Sucrose

Antibody Isotypes

CD3 FITC reagent: Mouse IgM
CD45RA RPE reagent: Mouse IgG1
CD161 A647 reagent: Mouse IgG1

Storage Information

Storage: Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of reconstitution

More Information

UniProt: P27471 Related reagents; A4KWA1 Related reagents; P19377 Related reagents; Q64159 Related reagents; P04157 Related reagents
Entrez Gene: Klrb1b Related reagents; Ptprc Related reagents; Cd3d Related reagents; Klrb1a Related reagents; Cd3g Related reagents
GO Terms: GO:0016021 integral to membrane; GO:0004872 receptor activity; GO:0005529 sugar binding; GO:0001915 negative regulation of T cell mediated cytotoxicity; GO:0001960 negative regulation of cytokine-mediated signaling pathway; GO:0005001 transmembrane receptor protein tyrosine phosphatase activity; GO:0005887 integral to plasma membrane; GO:0005925 focal adhesion; GO:0006469 negative regulation of protein kinase activity; GO:0006470 protein dephosphorylation; GO:0019887 protein kinase regulator activity; GO:0009898 internal side of plasma membrane; GO:0010332 response to gamma radiation; GO:0019901 protein kinase binding; GO:0042100 B cell proliferation; GO:0045121 membrane raft; GO:0050852 T cell receptor signaling pathway; GO:0050853 B cell receptor signaling pathway; GO:0050857 positive regulation of antigen receptor-mediated signaling pathway; GO:0051209 release of sequestered calcium ion into cytosol; GO:0051607 defense response to virus; GO:0004888 transmembrane receptor activity; GO:0007166 cell surface receptor linked signaling pathway; GO:0045059 positive thymic T cell selection; GO:0007163 establishment or maintenance of cell polarity; GO:0015031 protein transport; GO:0042981 regulation of apoptosis; GO:0046982 protein heterodimerization activity
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory: For research purposes only

Applications of CD3/CD45RA/CD161

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			Neat

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul

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