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CD3/CD4 | IF4 : W3/25

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Anti Rat CD3:FITC/CD4:RPE, clone IF4 : W3/25 thumbnail image 1 Anti Rat CD3:FITC/CD4:RPE, clone IF4 : W3/25 thumbnail image 2
Anti Rat CD3:FITC/CD4:RPE, clone IF4 : W3/25 gallery image 1
Staining of rat spleen cells with Mouse anti Rat CD3:FITC/CD4:RPE (DC041).
Anti Rat CD3:FITC/CD4:RPE, clone IF4 : W3/25 gallery image 2
Published customer image:
Dual color reagent Mouse anti Rat CD3 and CD4 antibodies (DC041) used for staining of rat lymphocyte subsets by flow cytometry
Image caption:
Mature CD4 or CD8 positive T lymphocytes in the peripheral blood of Rag1 mutant rats. A Absence of CD4+/CD8+ lymphocytes in wild type and Rag1 mutant rats. B and C Reduction of CD4+/CD3+ and CD8+/CD3+ T cells. D and E While there are no differences in the population of CD25+/CD4+ T cells the number of CD25+/CD8+ lymphocytes was reduced. orange: autofluorescent cells; green: FITC positive lymphocytes; blue: PE positive lymphocytes.

From: Zschemisch NH, Glage S, Wedekind D, Weinstein EJ, Cui X, Dorsch M, Hedrich HJ.
Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat.
BMC Immunol. 2012 Nov 8;13:60.

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Mouse anti Rat CD3:FITC/CD4:RPE

Product Type
Dual Color Reagent
Clone
IF4 : W3/25
Isotype
Cocktail
Specificity
CD3/CD4

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DC041
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Dual colour reagent Mouse anti Rat CD3 and CD4 antibodies is a combination of monoclonal antibodies recognizing rat CD3 and CD4 cell surface antigens.

Mouse anti Rat CD3 antibody, clone IF4 recognizes rat CD3, a cell surface glycoprotein expressed by mature T lymphocytes.

Mouse anti Rat CD4 antibody, clone W3/25 recognizes rat CD4, a ~55 kDa glycoprotein expressed by helper T lymphocytes, thymocytes and macrophages.

Target Species
Rat
Product Form
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Antibody Isotypes
FITC reagent: IgM (MOUSE)
RPE reagent: IgG1 (MOUSE)
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
RPE 488nm laser 496 578
RPE 561nm laser 546 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cell in 100ul.

References for CD3/CD4

  1. Tanaka, T. et al. (1989) Characterization of a CD3-like rat T cell surface antigen recognized by a monoclonal antibody.
    J Immunol. 142 (8): 2791-5.
  2. Barclay, A.N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
    Immunology. 42 (4): 593-600.
  3. Zschemisch, N.H. et al. (2012) Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat.
    BMC Immunol. 13: 60.
  4. Rivera de Jesus, E, et al. (2016) Adoptive Transfer of Dendritic Cells Expressing Fas Ligand Modulates Intestinal Inflammation in a Model of Inflammatory Bowel Disease.
    Journal of Clinical & Cellular Immunology. 7: 411.

RRID
AB_324613
UniProt
P19377
Q64159
P05540
Entrez Gene
Cd4
Cd3d
Cd3g
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0006955 immune response
GO:0019901 protein kinase binding
GO:0045058 T cell selection
GO:0004888 transmembrane receptor activity
GO:0007166 cell surface receptor linked signaling pathway
GO:0045059 positive thymic T cell selection
GO:0007163 establishment or maintenance of cell polarity
GO:0015031 protein transport
GO:0042981 regulation of apoptosis
GO:0046982 protein heterodimerization activity
View more products with CD3 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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