CD3/CD4 | IF4 : W3/25
Dual color reagent Mouse anti Rat CD3 and CD4 antibodies (DC041) used for staining of rat lymphocyte subsets by flow cytometry
Image caption:
Mature CD4 or CD8 positive T lymphocytes in the peripheral blood of Rag1 mutant rats. A Absence of CD4+/CD8+ lymphocytes in wild type and Rag1 mutant rats. B and C Reduction of CD4+/CD3+ and CD8+/CD3+ T cells. D and E While there are no differences in the population of CD25+/CD4+ T cells the number of CD25+/CD8+ lymphocytes was reduced. orange: autofluorescent cells; green: FITC positive lymphocytes; blue: PE positive lymphocytes.
From: Zschemisch NH, Glage S, Wedekind D, Weinstein EJ, Cui X, Dorsch M, Hedrich HJ.
Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat.
BMC Immunol. 2012 Nov 8;13:60.
Dual colour reagent Mouse anti Rat CD3 and CD4 antibodies is a combination of monoclonal antibodies recognizing rat CD3 and CD4 cell surface antigens.
Mouse anti Rat CD3 antibody, clone IF4 recognizes rat CD3, a cell surface glycoprotein expressed by mature T lymphocytes.
Mouse anti Rat CD4 antibody, clone W3/25 recognizes rat CD4, a ~55 kDa glycoprotein expressed by helper T lymphocytes, thymocytes and macrophages.
Mouse anti Rat CD3 antibody, clone IF4 recognizes rat CD3, a cell surface glycoprotein expressed by mature T lymphocytes.
Mouse anti Rat CD4 antibody, clone W3/25 recognizes rat CD4, a ~55 kDa glycoprotein expressed by helper T lymphocytes, thymocytes and macrophages.
Product Details
- Target Species
- Rat
- Product Form
- Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
- Reconstitution
- Reconstitute with 1 ml distilled water
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Antibody Isotypes
- FITC reagent: IgM (MOUSE)
RPE reagent: IgG1 (MOUSE)
Storage Information
- Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of reconstitution.
More Information
- UniProt
- P19377 Related reagents
- Q64159 Related reagents
- P05540 Related reagents
- Entrez Gene
- Cd4 Related reagents
- Cd3d Related reagents
- Cd3g Related reagents
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0019901 protein kinase binding
- GO:0045058 T cell selection
- GO:0004888 transmembrane receptor activity
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0045059 positive thymic T cell selection
- GO:0007163 establishment or maintenance of cell polarity
- GO:0015031 protein transport
- GO:0042981 regulation of apoptosis
- GO:0046982 protein heterodimerization activity
- Regulatory
- For research purposes only
Applications of CD3/CD4
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cell in 100ul.
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Product Specific References
References for CD3/CD4
-
Tanaka, T. et al. (1989) Characterization of a CD3-like rat T cell surface antigen recognized by a monoclonal antibody.
J Immunol. 142 (8): 2791-5. -
Barclay, A.N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
Immunology. 42 (4): 593-600. -
Zschemisch, N.H. et al. (2012) Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat.
BMC Immunol. 13: 60. -
Rivera de Jesus, E, et al. (2016) Adoptive Transfer of Dendritic Cells Expressing Fas Ligand Modulates Intestinal Inflammation in a Model of Inflammatory Bowel Disease.
Journal of Clinical & Cellular Immunology. 7: 411.
