CD3/CD4 | IF4 : W3/25

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Anti Rat CD3:FITC/CD4:RPE, clone IF4 : W3/25 thumbnail image 1

Anti Rat CD3:FITC/CD4:RPE, clone IF4 : W3/25 thumbnail image 2

Anti Rat CD3:FITC/CD4:RPE, clone IF4 : W3/25 gallery image 1 — Staining of rat spleen cells with Mouse anti Rat CD3:FITC/CD4:RPE (**DC041**).

Anti Rat CD3:FITC/CD4:RPE, clone IF4 : W3/25 gallery image 2 — **Published customer image:**
Dual color reagent Mouse anti Rat CD3 and CD4 antibodies (**DC041**) used for staining of rat lymphocyte subsets by flow cytometry
**Image caption:**
Mature CD4 or CD8 positive T lymphocytes in the peripheral blood of Rag1 mutant rats. A Absence of CD4+/CD8+ lymphocytes in wild type and Rag1 mutant rats. B and C Reduction of CD4⁺/CD3⁺ and CD8⁺/CD3⁺ T cells. D and E While there are no differences in the population of CD25⁺/CD4⁺ T cells the number of CD25⁺/CD8⁺ lymphocytes was reduced. orange: autofluorescent cells; green: FITC positive lymphocytes; blue: PE positive lymphocytes.

From: Zschemisch NH, Glage S, Wedekind D, Weinstein EJ, Cui X, Dorsch M, Hedrich HJ.
Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat.
BMC Immunol. 2012 Nov 8;13:60.

Mouse anti Rat CD3:FITC/CD4:RPE

Product Type: Dual Color Reagent
Clone: IF4 : W3/25
Isotype: Cocktail

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
DC041	F			100 Tests

Summary
References (4)

Dual colour reagent Mouse anti Rat CD3 and CD4 antibodies is a combination of monoclonal antibodies recognizing rat CD3 and CD4 cell surface antigens.

Mouse anti Rat CD3 antibody, clone IF4 recognizes rat CD3, a cell surface glycoprotein expressed by mature T lymphocytes.

Mouse anti Rat CD4 antibody, clone W3/25 recognizes rat CD4, a ~55 kDa glycoprotein expressed by helper T lymphocytes, thymocytes and macrophages.

Product Details

Target Species

Rat

Product Form

Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.

Reconstitution

Reconstitute with 1 ml distilled water

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin
5%	Sucrose

Antibody Isotypes

FITC reagent: IgM (MOUSE)
RPE reagent: IgG1 (MOUSE)

Storage Information

Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of reconstitution.

More Information

UniProt: P19377 Related reagents; Q64159 Related reagents; P05540 Related reagents
Entrez Gene: Cd4 Related reagents; Cd3d Related reagents; Cd3g Related reagents
GO Terms: GO:0007155 cell adhesion; GO:0016021 integral to membrane; GO:0006955 immune response; GO:0019901 protein kinase binding; GO:0045058 T cell selection; GO:0004888 transmembrane receptor activity; GO:0007166 cell surface receptor linked signaling pathway; GO:0045059 positive thymic T cell selection; GO:0007163 establishment or maintenance of cell polarity; GO:0015031 protein transport; GO:0042981 regulation of apoptosis; GO:0046982 protein heterodimerization activity
Regulatory: For research purposes only

Applications of CD3/CD4

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			Neat

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cell in 100ul.

Product Specific References

References for CD3/CD4

Tanaka, T. et al. (1989) Characterization of a CD3-like rat T cell surface antigen recognized by a monoclonal antibody.
J Immunol. 142 (8): 2791-5.
Barclay, A.N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
Immunology. 42 (4): 593-600.
Zschemisch, N.H. et al. (2012) Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat.
BMC Immunol. 13: 60.
Rivera de Jesus, E, et al. (2016) Adoptive Transfer of Dendritic Cells Expressing Fas Ligand Modulates Intestinal Inflammation in a Model of Inflammatory Bowel Disease.
Journal of Clinical & Cellular Immunology. 7: 411.

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