CD26 antibody | OX-61

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Mouse anti Rat CD26

Product Type
Monoclonal Antibody
Clone
OX-61
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA924R C F IF IP 0.25 mg
Mouse anti Rat CD26 antibody, clone OX-61 recognizes CD26, rat liver antigen dipeptidyl peptidase IV. Reacts with liver bile canicular domains and brush borders of bile ducts. It also recognizes other epithelial brush borders such as a kidney and intestine, MRC OX-61 recognizes T lymphocytes and some B lymphocytes.

Mouse anti Rat CD26 antibody, clone OX-61 has been reported as being suitable for use in Western blotting (McCaughan et al. 1990).

Product Details

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
110 kDa membrane glycoprotein
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the PS-NSI/1Ag4-1 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P14740 Related reagents
Entrez Gene
Dpp4 Related reagents
GO Terms
GO:0002020 protease binding
GO:0002709 regulation of T cell mediated immunity
GO:0004252 serine-type endopeptidase activity
GO:0004177 aminopeptidase activity
GO:0008239 dipeptidyl-peptidase activity
GO:0005102 receptor binding
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0005783 endoplasmic reticulum
GO:0005518 collagen binding
GO:0005624 membrane fraction
GO:0005625 soluble fraction
GO:0005794 Golgi apparatus
GO:0030139 endocytic vesicle
GO:0006508 proteolysis
GO:0008284 positive regulation of cell proliferation
GO:0009986 cell surface
GO:0010716 negative regulation of extracellular matrix disassembly
GO:0016324 apical plasma membrane
GO:0031258 lamellipodium membrane
GO:0031295 T cell costimulation
GO:0042277 peptide binding
GO:0042803 protein homodimerization activity
GO:0043542 endothelial cell migration
GO:0045121 membrane raft
GO:0051234 establishment of localization
GO:0071438 invadopodium membrane
Regulatory
For research purposes only

Applications of CD26 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/200
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul. Method sheets available on request.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2a Negative Control MCA1210 100 Tests F

Application Based External Images

Immunofluorescence

Product Specific References

References for CD26 antibody

  1. McCaughan, G.W. et al. (1990) Identification of the bile canalicular cell surface molecule GP110 as the ectopeptidase dipeptidyl peptidase IV: an analysis by tissue distribution, purification and N-terminal amino acid sequence.
    Hepatology. 11 (4): 534-44.
  2. Cohen, D. et al. (2004) Par-1 promotes a hepatic mode of apical protein trafficking in MDCK cells.
    Proc Natl Acad Sci U S A. 101 (38): 13792-7.
  3. Korom S et al. (1997) Inhibition of CD26/dipeptidyl peptidase IV activity in vivo prolongs cardiac allograft survival in rat recipients.
    Transplantation. 63 (10): 1495-500.
  4. Kraml J et al. (2003) Glucocorticoid agonistic and antagonistic effects of mifepristone and onapristone on thymocyte subset composition and CD26/dipeptidyl peptidase IV activity in infant male rats.
    J Steroid Biochem Mol Biol. 87 (1): 85-96.
  5. Cohen D et al. (2007) Par1b promotes hepatic-type lumen polarity in Madin Darby canine kidney cells via myosin II- and E-cadherin-dependent signaling.
    Mol Biol Cell. 18 (6): 2203-15.
  6. László, V. et al. (2008) Triiodothyronine accelerates differentiation of rat liver progenitor cells into hepatocytes.
    Histochem Cell Biol. 130 (5): 1005-14.
  7. Skripuletz T et al. (2007) Dose-dependent recruitment of CD25+ and CD26+ T cells in a novel F344 rat model of asthma.
    Am J Physiol Lung Cell Mol Physiol. 292 (6): L1564-71.
  8. Molina-Jijón E et al. (2015) All-trans retinoic acid prevents oxidative stress-induced loss of renal tight junction proteins in type-1 diabetic model.
    J Nutr Biochem. 26 (5): 441-54.
  9. Trujillo, J. et al. (2016) Curcumin prevents cisplatin-induced decrease in the tight and adherens junctions: relation to oxidative stress.
    Food Funct. 7 (1): 279-93.
  10. Paku, S. et al. (2004) 2-acetylaminofluorene dose-dependent differentiation of rat oval cells into hepatocytes: confocal and electron microscopic studies.
    Hepatology. 39 (5): 1353-61.
  11. Santoyo-Sánchez MP et al. (2013) Impaired endocytosis in proximal tubule from subchronic exposure to cadmium involves angiotensin II type 1 and cubilin receptors.
    BMC Nephrol. 14: 211.
  12. Molina-Jijón, E. et al. (2017) Aldosterone signaling regulates the over-expression of claudin-4 and -8 at the distal nephron from type 1 diabetic rats.
    PLoS One. 12 (5): e0177362.