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CD252 antibody | ATM-2

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Anti Rat CD252 Antibody, clone ATM-2 gallery image 1
Detection of recombinant Rat CD252 in ELISA using MCA4634 with Rabbit F(ab')2 anti Mouse IgG: HRP (Human adsorbed) (STAR13B) at 1/1000 dilution as the secondary antibody

Mouse anti Rat CD252

Product Type
Monoclonal Antibody
Clone
ATM-2
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA4634
Datasheet
E F*
SDS
0.25 mg loader

Product Details

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9Z2P3
Entrez Gene
Tnfsf4
GO Terms
GO:0001816 cytokine production
GO:0016021 integral to membrane
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006955 immune response
GO:0009986 cell surface
GO:0032743 positive regulation of interleukin-2 production
GO:0042098 T cell proliferation
GO:0042104 positive regulation of activated T cell proliferation
Regulatory
For research purposes only

Applications of CD252 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 50ug/ml 0.1ug/ml
Flow Cytometry 1
  1. 1Bio-Rad recommends a three layer detection system using STAR11B followed by STAR4A, to enhance the staining of CD252 with MCA4634.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA1209 F 0.1 mg loader

Product Specific References

Source Reference

  1. Satake, Y. et al. (2000) Characterization of rat OX40 ligand by monoclonal antibody.
    Biochem Biophys Res Commun. 270 (3): 1041-8.

References for CD252 antibody

  1. Yuan, X. et al. (2003) The role of the CD134-CD134 ligand costimulatory pathway in alloimmune responses in vivo.
    J Immunol. 170 (6): 2949-55.
  2. Macphee, I.A. et al. (2006) Blockade of OX40-ligand after initial triggering of the T helper 2 response inhibits mercuric chloride-induced autoimmunity.
    Immunology. 117 (3): 402-8.

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