CD252 antibody | ATM-2
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|Mouse anti Rat CD252 antibody, clone ATM-2 recognizes rat CD252, otherwise known as OX40L or CD134L, a ~22 kDa type II transmembrane glycoprotein and member of the TNF superfamily, expressed by antigen-presenting cells including activated dendritic cells and B cells, and also by vascular endothelium.
The interaction of CD252 with its receptor OX40/CD134, acts as a co-stimulator of T cell activation, proliferation and both Th1 and Th2 cytokine production, and is also important in activated B cell proliferation and secretion of immunoglobulins (Igs). CD252/OX40 interaction has been implicated in HTLV-1 associated diseases including myelopathy and adult T cell leukaemia, and also with the autoimmune diseases rheumatoid arthritis and inflammatory bowel disease.
Mouse anti Rat CD252 antibody, clone ATM-2 has been shown to block the co-stimulatory activity of CD252 on rat T cell proliferation.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- ACI-S1 rat T-cell lymphoma cell line.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Flow Cytometry 1|
- 1Bio-Rad recommends a three layer detection system using STAR11B followed by STAR4A, to enhance the staining of CD252 with MCA4634.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Satake, Y. et al. (2000) Characterization of rat OX40 ligand by monoclonal antibody.
Biochem Biophys Res Commun. 270 (3): 1041-8.
References for CD252 antibody
Yuan, X. et al. (2003) The role of the CD134-CD134 ligand costimulatory pathway in alloimmune responses in vivo.
J Immunol. 170 (6): 2949-55.
Macphee, I.A. et al. (2006) Blockade of OX40-ligand after initial triggering of the T helper 2 response inhibits mercuric chloride-induced autoimmunity.
Immunology. 117 (3): 402-8.
- OX40 Ligand
- Entrez Gene
- GO Terms
- GO:0001816 cytokine production
- GO:0016021 integral to membrane
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0009986 cell surface
- GO:0032743 positive regulation of interleukin-2 production
- GO:0042098 T cell proliferation
- GO:0042104 positive regulation of activated T cell proliferation
- View More GO Terms
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