CD252 antibody | ATM-2
Mouse anti Rat CD252 antibody, clone ATM-2 recognizes rat CD252, otherwise known as OX40L or CD134L, a ~22 kDa type II transmembrane glycoprotein and member of the TNF superfamily, expressed by antigen-presenting cells including activated dendritic cells and B cells, and also by vascular endothelium.
The interaction of CD252 with its receptor OX40/CD134, acts as a co-stimulator of T cell activation, proliferation and both Th1 and Th2 cytokine production, and is also important in activated B cell proliferation and secretion of immunoglobulins (Igs). CD252/OX40 interaction has been implicated in HTLV-1 associated diseases including myelopathy and adult T cell leukaemia, and also with the autoimmune diseases rheumatoid arthritis and inflammatory bowel disease.
Mouse anti Rat CD252 antibody, clone ATM-2 has been shown to block the co-stimulatory activity of CD252 on rat T cell proliferation.
The interaction of CD252 with its receptor OX40/CD134, acts as a co-stimulator of T cell activation, proliferation and both Th1 and Th2 cytokine production, and is also important in activated B cell proliferation and secretion of immunoglobulins (Igs). CD252/OX40 interaction has been implicated in HTLV-1 associated diseases including myelopathy and adult T cell leukaemia, and also with the autoimmune diseases rheumatoid arthritis and inflammatory bowel disease.
Mouse anti Rat CD252 antibody, clone ATM-2 has been shown to block the co-stimulatory activity of CD252 on rat T cell proliferation.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- ACI-S1 rat T-cell lymphoma cell line.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9Z2P3
- Entrez Gene
- Tnfsf4
- GO Terms
- GO:0001816 cytokine production
- GO:0016021 integral to membrane
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0009986 cell surface
- GO:0032743 positive regulation of interleukin-2 production
- GO:0042098 T cell proliferation
- GO:0042104 positive regulation of activated T cell proliferation
- Regulatory
- For research purposes only
Applications of CD252 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 50ug/ml | 0.1ug/ml | |
| Flow Cytometry 1 |
- 1Bio-Rad recommends a three layer detection system using STAR11B followed by STAR4A, to enhance the staining of CD252 with MCA4634.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
|
Product Specific References
Source Reference
-
Satake, Y. et al. (2000) Characterization of rat OX40 ligand by monoclonal antibody.
Biochem Biophys Res Commun. 270 (3): 1041-8.
References for CD252 antibody
-
Yuan, X. et al. (2003) The role of the CD134-CD134 ligand costimulatory pathway in alloimmune responses in vivo.
J Immunol. 170 (6): 2949-55. -
Macphee, I.A. et al. (2006) Blockade of OX40-ligand after initial triggering of the T helper 2 response inhibits mercuric chloride-induced autoimmunity.
Immunology. 117 (3): 402-8.
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