CD1d antibody | WTH1









Mouse anti Rat CD1d:Biotin
Mouse anti Rat CD1d:FITC
Mouse anti Rat CD1d
- Product Type
- Monoclonal Antibody
- Clone
- WTH1
- Isotype
- IgG2a
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA5799B | C E F IP WB* | 0.1 mg |
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MCA5799F | F | 0.1 mg |
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MCA5799GA | C E F FN* IP WB* | 0.1 mg |
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MCA5799 | C E F FN* IP WB* | 0.25 mg |
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Clone WTH1 binds with high affinity to the mature CD1d heavy chain independently of beta-2-microglobulin. Mouse anti Rat CD1d antibody, clone WTH1 efficiently blocks rat and mouse invariant natural killer T cell (iNKT cell) response to a-GalCer (an antigen for iNKT cells) and has a slightly higher affinity to native CD1d than clone WTH2.
Clone WTH1 cross reacts weakly with mouse tissue in IHC and western blotting, but reacts strongly with both rat and mouse antigens in flow cytometry and immunoprecipitation.
Product Details
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Cotton rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Immunogen
- Rat CD1d-transduced M12.4.1.C3 cells.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P11609
- Q63493
- Entrez Gene
- Cd1d1
- Cd1d1
- GO Terms
- GO:0005515 protein binding
- GO:0001916 positive regulation of T cell mediated cytotoxicity
- GO:0016021 integral to membrane
- GO:0005765 lysosomal membrane
- GO:0005769 early endosome
- GO:0005770 late endosome
- GO:0009897 external side of plasma membrane
- GO:0010008 endosome membrane
- GO:0030883 endogenous lipid antigen binding
- GO:0045059 positive thymic T cell selection
- GO:0045078 positive regulation of interferon-gamma biosynthetic process
- GO:0045086 positive regulation of interleukin-2 biosynthetic process
- GO:0045087 innate immune response
- GO:0045404 positive regulation of interleukin-4 biosynthetic process
- GO:0048007 antigen processing and presentation, exogenous lipid antigen via MHC class Ib
- GO:0051135 positive regulation of NK T cell activation
- GO:0051138 positive regulation of NK T cell differentiation
- GO:0042612 MHC class I protein complex
- Regulatory
- For research purposes only
Applications of CD1d antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
Flow Cytometry | 1/1,000 | ||
Immunohistology - Frozen | |||
Immunoprecipitation | |||
Western Blotting 1 | |||
Flow Cytometry | 1/1,000 | ||
ELISA | |||
Flow Cytometry | 1/2,000 | ||
Functional Assays 1 | |||
Immunohistology - Frozen | |||
Immunoprecipitation | |||
Western Blotting 2 |
- 1Clone WTH1 recognizes CD1d under non-reducing conditions, see Monzon-Casanova, E. et al. for details.
- 1 This antibody contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
- 2 Clone WTH1 recognizes CD1d under non-reducing conditions (Monzon-Casanova, E. et al.).
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG2a Negative Control:FITC | MCA1210F | F | 0.1 mg |
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Mouse IgG2a Negative Control | MCA1210 | F | 100 Tests |
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Product Specific References
References for CD1d antibody
-
Monzon-Casanova, E. et al. (2010) CD1d expression in paneth cells and rat exocrine pancreas revealed by novel monoclonal antibodies which differentially affect NKT cell activation.
PLoS One 5:e13089 -
Fichtner AS et al. (2015) Function and expression of CD1d and invariant natural killer T-cell receptor in the cotton rat (Sigmodon hispidus).
Immunology. 146 (4): 618-29. -
Monzon-Casanova, E. et al. (2013) Direct identification of rat iNKT cells reveals remarkable similarities to human iNKT cells and a profound deficiency in LEW rats.
Eur J Immunol. 43 (2): 404-15.
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