CD152 antibody | WKH203
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F ResetMouse anti Rat CD152
- Product Type
- Monoclonal Antibody
- Clone
- WKH203
- Isotype
- IgG1
- Specificity
- CD152
Antibody Quality and Performance Guarantee
| Mouse anti Rat CD152 antibody, clone WKH203 recognizes rat CD152, also known as cytoxic-T-lymphocyte antigen-4 (CTLA-4), which is similar in structure to CD28 and also binds ligands CD80 and CD86. CD152 is expressed by activated T-lymphocytes and by regulatory T-lymphocytes (Lin and Hunig 2003). |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Purified rCTLA-4hIg fusion protein.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c were fused with cells of the X63-Ag8-653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Flow Cytometry 1 | |
1/10 | 1/20 |
| Western Blotting | |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Lin, C.H. & Hünig, T. (2003) Efficient expansion of regulatory T cells in vitro and in vivo with a CD28 superagonist.
Eur J Immunol. 33 (3): 626-38.
References for CD152 antibody
-
Elflein, K. et al. (2003) Rapid recovery from T lymphopenia by CD28 superagonist therapy.
Blood. 102 (5): 1764-70. -
Blewett, H.J. et al. (2009) Vaccenic acid favourably alters immune function in obese JCR:LA-cp rats.
Br J Nutr. 102 (4): 526-36. -
Almolda, B. et al. (2010) Activated microglial cells acquire an immature dendritic cell phenotype and may terminate the immune response in an acute model of EAE.
J Neuroimmunol. 223 (1-2): 39-54. -
Beyersdorf, N. et al. (2005) Selective targeting of regulatory T cells with CD28 superagonists allows effective therapy of experimental autoimmune encephalomyelitis.
J Exp Med. 202 (3): 445-55. -
Elflein, K. et al. (2003) Rapid recovery from T lymphopenia by CD28 superagonist therapy.
Blood. 102 (5): 1764-70. -
Hara, Y. et al. (2006) Anergic lymphocytes generated by blocking CD28 and ICOS pathways in vitro prolong rat cardiac graft survival.
Int Immunopharmacol. 6 (7): 1143-51.
- Synonyms
- CLTA-4
- CTLA-4
- RRID
- AB_2087798
MCA2092
150545
155755
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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