CD147 antibody | OX-47

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Mouse anti Rat CD147:FITC

Mouse anti Rat CD147:RPE

Mouse anti Rat CD147

Product Type
Monoclonal Antibody
Clone
OX-47
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA729F F 0.1 mg
MCA729PE F 100 Tests
MCA729 C F IF WB 2 ml
Mouse anti Rat CD147 antibody, clone OX-47 recognizes a membrane glycoprotein of ~42-48 kDa expressed at high levels on activated lymphocytes but lower levels on resting cells (Paterson et al. 1987). CD147 is also present on some endothelia (Fossum et al. 1991). The OX-47 antigen contains two Ig-like domains and an unusual transmembrane domain with a charged residue (Fossum et al. 1991).

CD147 is abundant on activated lymphocytes: it is the homologue of the chicken blood brain barrier antigen HT7.

Mouse anti Rat CD147 antibody, clone OX-47 is suitable for use in Western blotting (Foda et al. 2001).

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Product Form
Tissue Culture Supernatant - liquid
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Preparation
Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
T blasts from a mixed lymphocyte reaction.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells from the NS0/1 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P26453 Related reagents
Entrez Gene
Bsg Related reagents
GO Terms
GO:0005515 protein binding
GO:0005739 mitochondrion
GO:0016021 integral to membrane
GO:0005537 mannose binding
GO:0007566 embryo implantation
GO:0046689 response to mercury ion
GO:0042383 sarcolemma
GO:0042470 melanosome
GO:0042475 odontogenesis of dentine-containing tooth
GO:0043434 response to peptide hormone stimulus
GO:0045121 membrane raft
GO:0046697 decidualization
GO:0051591 response to cAMP
Regulatory
For research purposes only

Applications of CD147 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry Neat
Flow Cytometry Neat 1/20
Immunofluorescence
Immunohistology - Frozen Neat 1/20
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:FITC MCA1209F 0.1 mg F
Mouse IgG1 Negative Control:RPE MCA1209PE 100 Tests F
Mouse IgG1 Negative Control MCA1209 0.1 mg F

Application Based External Images

Immunofluorescence

Immunohistology - Frozen

Product Specific References

References for CD147 antibody

  1. Paterson DJ et al. (1987) Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts.
    Mol Immunol. 24 (12): 1281-90.
  2. Fossum, S. et al. (1991) The MRC OX-47 antigen is a member of the immunoglobulin superfamily with an unusual transmembrane sequence.
    Eur J Immunol. 21 (3): 671-9.
  3. Cassella, J.P. et al. (1996) Ontogeny of four blood-brain barrier markers: an immunocytochemical comparison of pial and cerebral cortical microvessels.
    J Anat. 189 ( Pt 2): 407-15.
  4. Foda, H.D. et al. (2001) Ventilator-induced lung injury upregulates and activates gelatinases and EMMPRIN: attenuation by the synthetic matrix metalloproteinase inhibitor, Prinomastat (AG3340).
    Am J Respir Cell Mol Biol. 25 (6): 717-24.
  5. Stevenson, K.S. et al. (2009) Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations.
    Stem Cells Dev. 18: 1389-98.
  6. Betsuyaku, T. et al. (2003) Increased basigin in bleomycin-induced lung injury.
    Am J Respir Cell Mol Biol. 28: 600-6.
  7. Du, Y. et al. (2008) Synthetic sandwich culture of 3D hepatocyte monolayer.
    Biomaterials. 29: 290-301.
  8. Nakao, Y. et al. (2011) Bile canaliculi formation by aligning rat primary hepatocytes in a microfluidic device.
    Biomicrofluidics. 5: 22212.
  9. Nugraha, B. et al. (2011) Galactosylated cellulosic sponge for multi-well drug safety testing.
    Biomaterials. 32: 6982-94.
  10. Xia, L. et al. (2009) Laminar-flow immediate-overlay hepatocyte sandwich perfusion system for drug hepatotoxicity testing.
    Biomaterials. 30: 5927-36.
  11. Zhang, S. et al. (2008) Microfabricated silicon nitride membranes for hepatocyte sandwich culture.
    Biomaterials. 29: 3993-4002.
  12. Cassella, J.P. et al. (1996) Ontogeny of four blood-brain barrier markers: an immunocytochemical comparison of pial and cerebral cortical microvessels.
    J Anat. 189: 407-15.
  13. Zhu, L. et al. (2016) Vertical-flow Bioreactor Array Compacts Hepatocytes for Enhanced Polarity and Functions
    Lab Chip. Sep 6 [Epub ahead of print]