CD147 antibody | OX-47

CD147 is abundant on activated lymphocytes: it is the homologue of the chicken blood brain barrier antigen HT7.
Mouse anti Rat CD147 antibody, clone OX-47 is suitable for use in Western blotting (Foda et al. 2001).
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Product Form
- Tissue Culture Supernatant - liquid
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- T blasts from a mixed lymphocyte reaction.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells from the NS0/1 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P26453
- Entrez Gene
- Bsg
- GO Terms
- GO:0005515 protein binding
- GO:0005739 mitochondrion
- GO:0016021 integral to membrane
- GO:0005537 mannose binding
- GO:0007566 embryo implantation
- GO:0046689 response to mercury ion
- GO:0042383 sarcolemma
- GO:0042470 melanosome
- GO:0042475 odontogenesis of dentine-containing tooth
- GO:0043434 response to peptide hormone stimulus
- GO:0045121 membrane raft
- GO:0046697 decidualization
- GO:0051591 response to cAMP
- Regulatory
- For research purposes only
Applications of CD147 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 | |
Flow Cytometry | Neat | ||
Flow Cytometry | Neat | 1/20 | |
Immunofluorescence | |||
Immunohistology - Frozen | Neat | 1/20 | |
Western Blotting |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control:FITC | MCA1209F | F | 0.1 mg |
![]() |
|
Mouse IgG1 Negative Control:RPE | MCA1209PE | F | 100 Tests |
![]() |
|
Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
![]() |
Product Specific References
References for CD147 antibody
-
Paterson DJ et al. (1987) Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts.
Mol Immunol. 24 (12): 1281-90. -
Fossum, S. et al. (1991) The MRC OX-47 antigen is a member of the immunoglobulin superfamily with an unusual transmembrane sequence.
Eur J Immunol. 21 (3): 671-9. -
Cassella, J.P. et al. (1996) Ontogeny of four blood-brain barrier markers: an immunocytochemical comparison of pial and cerebral cortical microvessels.
J Anat. 189 ( Pt 2): 407-15. -
Foda, H.D. et al. (2001) Ventilator-induced lung injury upregulates and activates gelatinases and EMMPRIN: attenuation by the synthetic matrix metalloproteinase inhibitor, Prinomastat (AG3340).
Am J Respir Cell Mol Biol. 25 (6): 717-24. -
Stevenson, K.S. et al. (2009) Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations.
Stem Cells Dev. 18: 1389-98. -
Betsuyaku, T. et al. (2003) Increased basigin in bleomycin-induced lung injury.
Am J Respir Cell Mol Biol. 28: 600-6. -
Du, Y. et al. (2008) Synthetic sandwich culture of 3D hepatocyte monolayer.
Biomaterials. 29: 290-301. -
Nakao, Y. et al. (2011) Bile canaliculi formation by aligning rat primary hepatocytes in a microfluidic device.
Biomicrofluidics. 5: 22212. -
Nugraha, B. et al. (2011) Galactosylated cellulosic sponge for multi-well drug safety testing.
Biomaterials. 32: 6982-94. -
Xia, L. et al. (2009) Laminar-flow immediate-overlay hepatocyte sandwich perfusion system for drug hepatotoxicity testing.
Biomaterials. 30: 5927-36. -
Zhang, S. et al. (2008) Microfabricated silicon nitride membranes for hepatocyte sandwich culture.
Biomaterials. 29: 3993-4002. -
Cassella, J.P. et al. (1996) Ontogeny of four blood-brain barrier markers: an immunocytochemical comparison of pial and cerebral cortical microvessels.
J Anat. 189: 407-15. -
Zhu, L. et al. (2016) A vertical-flow bioreactor array compacts hepatocytes for enhanced polarity and functions.
Lab Chip. 16 (20): 3898-908.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra