CD122 antibody | L316

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Mouse anti Rat CD122:FITC

Product Type
Monoclonal Antibody
Clone
L316
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2089F F* 0.1 mg
Mouse anti Rat CD122 antibody, clone L316 recognizes CD122 the 80 kDa Beta subunit of IL-2R, which is shared by the IL-15 receptor. The interleukin-2 receptor (IL-2R) is composed of three subunits, namely alpha, beta and gamma. In peripheral lymphoid tissues of adult rats, CD122 is primarily expressed by NK cells and a subset of T lymphocytes.

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Rat CRNK cells and rat IL-2R beta cDNA-transfected mouse L929 cell line.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P26896 Related reagents
Entrez Gene
Il2rb Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0004911 interleukin-2 receptor activity
GO:0030101 natural killer cell activation
Regulatory
For research purposes only

Applications of CD122 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat
  1. 1 An incubation time of 4 hours with MCA2089XZ is recommended for optimum staining, see Park et al.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:FITC MCA1209F 0.1 mg F

Application Based External Images

Functional Assays

Product Specific References

References for CD122 antibody

  1. Park JH et al. (1996) Identification and cellular distribution of the rat interleukin-2 receptor beta chain: induction of the IL-2R alpha- beta+ phenotype by major histocompatibility complex class I recognition during T cell development in vivo and by T cell receptor stimulation of CD4+8+ immature thymocytes in vitro.
    Eur J Immunol. 26 (10): 2371-5.
  2. Wrenshall, L.E. et al. (2014) Identification of a cytotoxic form of dimeric interleukin-2 in murine tissues.
    PLoS One. 9: e102191.