Caspr1 antibody | AbD06152

100% Secure


Human anti Caspr1

Product Type
Monoclonal Antibody
Clone
AbD06152
Isotype
HuCAL Fab bivalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA091 C E IF WB 0.1 mg
Human anti Caspr1 antibody, clone AbD06152 recognizes rat contactin-associated protein 1 (Caspr1), also known as neurexin 4 and paranodin, a ~190 kDa axonal transmembrane molecule. Caspr1 is a primarily expressed in myelinated nerves of the central and peripheral nervous system. It is a constituent of paranodal regions flanking the nodes of Ranvier and is believed to play an important role in paranodal junction formation.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Human Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilized.
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Fusion protein corresponding to the C-terminal region of rat Caspr1.
Approx. Protein Concentrations
Ig concentration 0.1 mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of reconstitution

More Information

UniProt
P97846 Related reagents
O54991 Related reagents
Entrez Gene
Cntnap1 Related reagents
Cntnap1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005102 receptor binding
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0007165 signal transduction
GO:0017124 SH3 domain binding
GO:0031175 neuron projection development
GO:0033270 paranode region of axon
GO:0042552 myelination
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of Caspr1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Frozen
Western Blotting 1/100 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.
Histology Positive Control Tissue
Mouse brain
Western Blotting
HCA091 detects a band of approximately 190 kDa in mouse brain cell lysates

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Histidine Tag:Alexa Fluor® 488 MCA1396A488 100 Tests/1ml F
Mouse anti Histidine Tag:Alexa Fluor® 647 MCA1396A647 1 ml F
Mouse anti Histidine Tag:Biotin MCA1396B 0.1 mg E WB
Mouse anti Histidine Tag:FITC MCA1396F 0.1 mg F IF
Mouse anti c-Myc:Alk.Phos. MCA2200A 0.1 mg C E P WB*
Mouse anti c-Myc:Alexa Fluor® 488 MCA2200A488 100 Tests/1ml F*
Mouse anti c-Myc:Alexa Fluor® 647 MCA2200A647 100 Tests/1ml F*
Mouse anti c-Myc:Biotin MCA2200B 0.1 mg C E P WB*
Mouse anti c-Myc:FITC MCA2200F 0.1 mg F*
Mouse anti c-Myc:HRP MCA2200P 0.1 mg C E P WB*
Mouse anti Penta Histidine Tag:HRP MCA5995P 125 µl E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Histar Detection System STAR3000B 150 Tests C P

Product Specific References

References for Caspr1 antibody

  1. Çolakoğlu, G. et al. (2014) Contactin-1 regulates myelination and nodal/paranodal domain organization in the central nervous system.
    Proc Natl Acad Sci USA. Jan 2.
Op-in to our ecommunications and set your preferences to start receiving resources, protocols, product updates, special offers, and more.