Alpha Internexin antibody | ID2

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Mouse anti Rat Alpha Internexin

Product Type
Monoclonal Antibody
Clone
ID2
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA4749
Datasheet
C IF P WB
MSDS
0.1 ml loader

Mouse anti Rat alpha Internexin antibody, clone 1D2 recognizes rat alpha internexin, also known as 66 kDa neurofilament protein, Neurofilament 5 or NF66. Alpha internexin is a 505 amino acid ~66 kDa class IV neuronal intermediate filament. It is a major component of the intermediate filament network in small interneurons and cerebellar granule cells, where it is present in parallel fibers. It is highly expressed in early neuronal development and declines into adulthood. There is also a marked up-regulation of alpha internexin during neuronal regeneration.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cat
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.065% Sodium Azide (NaN3)
Immunogen
Recombinant rat alpha Internexin expressed in and purified from E.coli.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P23565 Related reagents
Entrez Gene
Ina Related reagents
GO Terms
GO:0005198 structural molecule activity
GO:0007399 nervous system development
GO:0030154 cell differentiation
GO:0042246 tissue regeneration
Regulatory
For research purposes only

Applications of Alpha Internexin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/250
Immunohistology - Frozen 1/1000
Immunohistology - Paraffin 1/1000
Western Blotting 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA4749 detects a band of approximately 66kDa in rat cortex lysate.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for Alpha Internexin antibody

  1. Evans, J. et al. (2002) Characterization of mitotic neurons derived from adult rat hypothalamus and brain stem.
    J Neurophysiol. 87: 1076-85