Ig antibody | 2C7

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Mouse anti Rainbow Trout Ig

Product Type
Monoclonal Antibody
Clone
2C7
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5976 C E IF WB 0.5 ml
Mouse anti Rainbow Trout Ig, clone 2C7 recognizes immunoglobulin heavy chain from Rainbow Trout (Oncorhynchus mykiss), additionally clone 2C7 recognizes Ig from closely related piscine species belonging to the Oncorhynchus genera but not from Atlantic salmon (Salmo salar) (Kibenge, et. al. 2002) or more distantly related species such as Zebra Fish (Danio rerio).

Rainbow trout is one of a number of salmonid fish species widely used in the aquaculture industry in many countries. As with other farmed salmonid species, the close proximity of many individuals in a confined area leads to increased incidences of disease caused by a range of fungal, protozoan, bacterial and viral pathogens. The development of vaccine and treatment regimes is essential to combat these infections.

Clone 2C7 may be used to detect bacterial and viral infections in trout and salmon, and to identify the presence of unknown parasites indirectly by measurement and monitoring of serum immunoglobulins. Monoclonal antibodies specific to salmonid Ig have been demonstrated to be of use in measuring the efficacy of salmonid vaccines (Rømer Villumsen, et. al. 2012 and Chettri, et. al. 2013).

Product Details

Target Species
Rainbow Trout
Species Cross-Reactivity
Target SpeciesCross Reactivity
Atlantic Salmon
Chinnock Salmon
Chum Salmon
Coho Salmon
Sockeye Salmon
Zebrafish
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid (concentrated)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Rainbow Trout heavy and light chain immunoglobulins
Approx. Protein Concentrations
Ig concentration 0.2 - 0.5 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse NSO myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Ig antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Frozen
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Salmonid Ig AHP761 50 µl E IF WB
Mouse anti Salmonid Ig MCA2182 0.5 ml E F IF WB

Product Specific References

References for Ig antibody

  1. Kibenge, M.T (2002) Serological evidence of infectious salmon anaemia virus (ISAV) infection in farmed fishes, using an indirect enzyme-linked immunosorbent assay (ELISA).
    Dis. Aquat. Org. 51: 1-11.

Further Reading

  1. Raida, M.K. et al. (2011) Association between plasma antibody response and protection in rainbow trout Oncorhynchus mykiss immersion vaccinated against Yersinia ruckeri.
    PLoS One. 6: e18832.
  2. von Gersdorff Jørgensen, L. et al. (2011) Experimental evidence for direct in situ binding of IgM and IgT to early trophonts of Ichthyophthirius multifiliis (Fouquet) in the gills of rainbow trout, Oncorhynchus mykiss (Walbaum).
    J Fish Dis. 34: 749-55.
  3. Rømer Villumsen, K. et al. (2012) Potential Role of Specific Antibodies as Important Vaccine Induced Protective Mechanism against Aeromonas salmonicida in Rainbow Trout.
    PLoS One. 7(10): e46733.
  4. Skov, J. et al. (2012) Immunomodulatory effects of dietary β-1,3-glucan from Euglena gracilis in rainbow trout (Oncorhynchus mykiss) immersion vaccinated against Yersinia ruckeri.
    Fish Shellfish Immunol. 33: 111-20.
  5. Deshmukh, S. et al. (2013) Insight from Molecular, Pathological, and Immunohistochemical Studies on Cellular and Humoral Mechanisms Responsible for Vaccine-Induced Protection of Rainbow Trout against Yersinia ruckeri.
    Clin Vaccine Immunol. 20: 1623-41.
  6. Chettri, J.K. et al. (2013) Comparative evaluation of administration methods for a vaccine protecting rainbow trout against Yersinia ruckeri O1 biotype 2 infections.
    Vet Immunol Immunopathol. 154: 42-7.
  7. von Gersdorff Jørgensen. L, et al. (2012) Approaches towards DNA vaccination against a skin ciliate parasite in fish.
    PLoS One. 7: e48129.
  8. Holten-Andersen, L. et al. (2012) Determining vaccination frequency in farmed rainbow trout using Vibrio anguillarum O1 specific serum antibody measurements.
    PLoS One. 7: e49672.