Ig antibody | 2C7
Rainbow trout is one of a number of salmonid fish species widely used in the aquaculture industry in many countries. As with other farmed salmonid species, the close proximity of many individuals in a confined area leads to increased incidences of disease caused by a range of fungal, protozoan, bacterial and viral pathogens. The development of vaccine and treatment regimes is essential to combat these infections.
Clone 2C7 may be used to detect bacterial and viral infections in trout and salmon, and to identify the presence of unknown parasites indirectly by measurement and monitoring of serum immunoglobulins. Monoclonal antibodies specific to salmonid Ig have been demonstrated to be of use in measuring the efficacy of salmonid vaccines (Rømer Villumsen, et. al. 2012 and Chettri, et. al. 2013).
- Target Species
- Rainbow Trout
- Species Cross-Reactivity
Target Species Cross Reactivity Atlantic Salmon Chinnock Salmon Chum Salmon Coho Salmon Sockeye Salmon Zebrafish
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Tissue Culture Supernatant - liquid (concentrated)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Rainbow Trout heavy and light chain immunoglobulins
- Approx. Protein Concentrations
- Ig concentration 0.2 - 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse NSO myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- For research purposes only
Applications of Ig antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
Secondary Antibodies Available
Useful Reagents Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Rabbit anti Salmonid Ig||AHP761||E IF WB||50 µl|
|Mouse anti Salmonid Ig||MCA2182||E F IF WB||0.5 ml|
Product Specific References
References for Ig antibody
Kibenge, M.T (2002) Serological evidence of infectious salmon anaemia virus (ISAV) infection in farmed fishes, using an indirect enzyme-linked immunosorbent assay (ELISA).
Dis. Aquat. Org. 51: 1-11.
Raida, M.K. et al. (2011) Association between plasma antibody response and protection in rainbow trout Oncorhynchus mykiss immersion vaccinated against Yersinia ruckeri.
PLoS One. 6: e18832.
von Gersdorff Jørgensen, L. et al. (2011) Experimental evidence for direct in situ binding of IgM and IgT to early trophonts of Ichthyophthirius multifiliis (Fouquet) in the gills of rainbow trout, Oncorhynchus mykiss (Walbaum).
J Fish Dis. 34: 749-55.
Rømer Villumsen, K. et al. (2012) Potential Role of Specific Antibodies as Important Vaccine Induced Protective Mechanism against Aeromonas salmonicida in Rainbow Trout.
PLoS One. 7(10): e46733.
Skov, J. et al. (2012) Immunomodulatory effects of dietary β-1,3-glucan from Euglena gracilis in rainbow trout (Oncorhynchus mykiss) immersion vaccinated against Yersinia ruckeri.
Fish Shellfish Immunol. 33: 111-20.
Deshmukh, S. et al. (2013) Insight from Molecular, Pathological, and Immunohistochemical Studies on Cellular and Humoral Mechanisms Responsible for Vaccine-Induced Protection of Rainbow Trout against Yersinia ruckeri.
Clin Vaccine Immunol. 20: 1623-41.
Chettri, J.K. et al. (2013) Comparative evaluation of administration methods for a vaccine protecting rainbow trout against Yersinia ruckeri O1 biotype 2 infections.
Vet Immunol Immunopathol. 154: 42-7.
von Gersdorff Jørgensen. L, et al. (2012) Approaches towards DNA vaccination against a skin ciliate parasite in fish.
PLoS One. 7: e48129.
Holten-Andersen, L. et al. (2012) Determining vaccination frequency in farmed rainbow trout using Vibrio anguillarum O1 specific serum antibody measurements.
PLoS One. 7: e49672.