PARP1-S499-ADP-RIBOSE antibody | AbD34251
Anti-PARP1-S499-ADP-ribose antibody, clone AbD34251 (TZA022) used to detect PARP1, ADP-ribosylated at Serine 499 by western blotting.
Image caption:
Dependence of mono-ADPr on HPF1 and validation of live cell imaging probes.
(A) Immunoblotting images showing the mono-ADPr specificity of AbD33205, AbD34251, and AbD33644. WT U2OS cells were treated with either DMSO or 1 μM PARGi for 20 min followed by 2 mM H2O2 for 10min and lysed in 4% SDS lysis buffer.
From: Longarini EJ, Dauben H, Locatelli C, Wondisford AR, Smith R, Muench C, Kolvenbach A, Lynskey ML, Pope A, Bonfiglio JJ, Jurado EP, Fajka-Boja R, Colby T, Schuller M, Ahel I, Timinszky G, O'Sullivan RJ, Huet S, Matic I.
Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling.
Mol Cell. 2023 May 18;83(10):1743-1760.e11.
doi: 10.1016/j.molcel.2023.03.027.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
anti PARP1-S499-ADP-RIBOSE
- Product Type
- Monoclonal Antibody
- Clone
- AbD34251
- Isotype
- Fab antibody
- Specificity
- PARP1-S499-ADP-RIBOSE
Antibody Quality and Performance Guarantee
| Anti-PARP1-S499-ADP-ribose antibody, clone AbD34251, recognizes PARP1, ADP-ribosylated at Serine 499. Poly(ADP-ribose) polymerase 1 (PARP1) is an early responder to DNA damage in human cells. Upon binding to genomic lesions PARP1 is able to transfer a mono- or poly-ADP-ribose residue from nicotinamide adenine dinucleotide (NAD+) to their substrate proteins, for chromatin decompaction and repair factor recruitment (Bütepage et al. 2015, Suskiewicz et al. 2020). During the DNA damage response, serine is the primary target for PARP1 ADP-ribosylation which uses Histone PARylation factor 1 (HPF1) as an accessory factor to switch the amino-acid specificity of PARP1 from aspartate/glutamate to serine residues (Bonfiglio et al. 2017, Suskiewicz et al. 2020). PARP1/HPF1 during DNA damage response, primarily target PARP1 itself and histone H3, ribosylating these substrate proteins. This anti-PARP1-S499-ADP-ribose antibody, clone AbD34251 was generated using an ADP-ribosylated PARP1S499 antigen. It is tagged with a SpyTag2 at the C-terminus of the Fab heavy chain, enabling the user to couple it to a SpyCatcher reagent for conversion to alternative formats in less than an hour. It has the same specificity than the human/rabbit IgG chimera antibody HCA356, clone AbD34251 that does not bind any of the other Ser-ADP-ribosylated peptides tested, nor does it bind poly-ADP-ribose in a site-specific manner (Bonfiglio et al. 2020). |
- Target Species
- Protein/peptide tag
- Product Form
- A monovalent human recombinant Fab selected from the HuCAL® phage display library, Expressed in a proprietary E. coli. strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Recombinant Fab antibody expressed in E. coli and purified by affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Carrier Free
- Yes
- Immunogen
- APRGKS(ADPr)GAALSKKSKGQVGGK
- Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for PARP1-S499-ADP-RIBOSE antibody
-
Longarini, E.J. et al. (2023) Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling.
Mol Cell. 83 (10): 1743-1760.e11.
TZA022
162597
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