PAN-ADP-RIBOSE antibody | AbD33641

anti PAN-ADP-RIBOSE

Product Type
Monoclonal Antibody
Clone
AbD33641
Isotype
HuCAL Fab bivalent
Specificity
PAN-ADP-RIBOSE

Product Code Applications Pack Size List Price Your Price Qty
HCA353
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Anti pan-ADP-ribose antibody, clone AbD33641, recognizes both mono- and poly-adenosine diphosphate (ADP)-ribosylation. ADP-ribosylation is a reversible post-translational modification that occurs in multicellular organisms as well as some lower unicellular eukaryotes, but is absent in prokaryotes and yeast (Bürkle 2005). ADP ribosylation has been shown to play critical roles in many physiological and pathological processes, including bacterial pathogenesis and signaling and metabolism to control chromatin-related processes including transcription and DNA repair (Bonfiglio et al. 2020, Bütepage et al. 2015).

Members of the ADP-ribosyltransferase (ART) superfamily of proteins catalyze the transfer of ADP-ribose from nicotinamide adenine dinucleotide (NAD+) onto substrate protein via N-, O-, or S-glycosidic linkages. These transferases are able to transfer a single ADP-ribose residue to their substrate proteins, in a process known as mono-ADP-ribosylation. They are also able to attach additional ADP-ribose residues to create either linear or branched chains of ADP-ribose poly-ADP-ribosylation (Bütepage et al. 2015).

Anti pan-ADP-ribose antibody, clone AbD33641 has been used in ELISAs and Western blotting against free poly-ADP-ribose and free mono-ADP-ribose to characterise the specificity of this clone (Bonfiglio et al. 2020).

Target Species
Protein/peptide tag
Product Form
A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library. Expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Carrier Free
Yes
Immunogen
ARTKQTARKS(ADPr)TGGKAC
Approx. Protein Concentrations
Total protein concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 2 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
anti Mono-ADP-Ribose HCA354 E IF WB 0.1 mg loader
List Price Your Price
loader
Description anti Mono-ADP-Ribose
anti Mono-ADP-Ribose HCA355 IF WB 0.1 mg loader
List Price Your Price
loader
Description anti Mono-ADP-Ribose

Antibody Characterization Reference

  1. Bonfiglio, J.J. et al. (2020) An HPF1/PARP1-Based Chemical Biology Strategy for Exploring ADP-Ribosylation.
    Cell. 183 (4): 1086-1102.e23. Cell. 183 (4): 1086-1102.e23.