Mono-ADP-Ribose antibody | AbD43647ad

anti Mono-ADP-Ribose

Product Type
Monoclonal Antibody
Clone
AbD43647ad
Isotype
Fab antibody
Specificity
Mono-ADP-Ribose

Product Code Applications Pack Size List Price Your Price Qty
New TZA020
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E IF WB 0.1 mg
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Anti mono-ADP-ribose antibody, clone AbD43647ad, recognizes mono-adenosine diphosphate (ADP)-ribosylation. ADP-ribosylation is a reversible post-translational modification that occurs in multicellular organisms as well as some lower unicellular eukaryotes, but is absent in prokaryotes and yeast (Bürkle 2005). ADP ribosylation has been shown to play critical roles in many physiological and pathological processes, including bacterial pathogenesis and signaling and metabolism to control chromatin-related processes including transcription and DNA repair (Bonfiglio et al. 2020, Bütepage et al. 2015).

Members of the ADP-ribosyltransferase (ART) superfamily of proteins including the poly(ADP-ribose) polymerases (PARPs) subfamily, catalyze the transfer of ADP-ribose from nicotinamide adenine dinucleotide (NAD+) onto substrate protein via N-, O-, or S-glycosidic linkages. These transferases are able to transfer a single ADP-ribose residue to their substrate proteins, in a process known as mono-ADP-ribosylation (Bütepage et al. 2015).

The anti mono-ADP-ribose antibody, clone AbD43647ad is a mono-selective ADP-ribose antibody with a preference for mono-Ser-ADPr catalysed by the poly(ADP-ribose) polymerase 1 PARP1. Clone AbD43647ad is tagged with a SpyTag2 at the C-terminus of the Fab heavy chain, enabling the user to couple it to a SpyCatcher reagent for conversion to alternative formats in less than an hour.

This clone AbD43647ad is a mono-selective ADP-ribose antibody which was generated using an H3S10ADPr peptide as the antigen. This clone is specific for mono-ADPr and has a preference for Ser-mono-ADPr.

Target Species
Protein/peptide tag
Product Form
A monovalent human recombinant Fab selected from the HuCAL® phage display library, Expressed in a proprietary E. coli. strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Recombinant Fab antibody expressed in E. coli and purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.0095% MIT
Carrier Free
Yes
Immunogen
ARTKQTARKS(adpr)TGGKAPRKQLAGGK
Approx. Protein Concentrations
Total protein concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.