Protein Kinase C Epsilon antibody | 17

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Mouse anti Protein Kinase C Epsilon

Product Type
Monoclonal Antibody
Clone
17
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1574 C WB 2 ml
Mouse anti Protein Kinase C epsilon antibody, clone 17 recognizes the epsilon isoform of protein kinase C, known as Protein kinase C epsilon type, nPKC-epsilon or PKCε. PKCε is a 737 amino acid cytoplasmic serine/threonine-protein kinase containing an AGC-kinase C-terminal domain, a C2 domain, a protein kinase domain and 2 DAG-type zinc fingers

Mouse anti Protein Kinase C epsilon antibody, clone 17 recognizes the sequence NQEEFKGFSYFGEDLMP at the C terminus of PKC epsilon.

Product Details

Species Cross-Reactivity
Target SpeciesCross Reactivity
Mammals
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Protein kinase C-epsilon.
Fusion Partners
Spleen cells from immunized mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q02156 Related reagents
Entrez Gene
PRKCE Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005829 cytosol
GO:0004871 signal transducer activity
GO:0005783 endoplasmic reticulum
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
GO:0006917 induction of apoptosis
GO:0007202 activation of phospholipase C activity
GO:0008047 enzyme activator activity
GO:0019899 enzyme binding
GO:0030168 platelet activation
GO:0048011 nerve growth factor receptor signaling pathway
Regulatory
For research purposes only

Applications of Protein Kinase C Epsilon antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen Nt - 1/10
Western Blotting 1/10 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F