Polyubiquitin antibody | FK1
Ubiquitin, as the name implies, is a ubiquitously expressed and highly conserved protein of 8.6 kDa. The protein is covalently linked to selected lysine residues in a post-translational modification processes known as ubiquitylation or ubiquitination. This chemical reaction is mediated by three different protein families; ubiquitin-activating enzymes (also known as E1s), ubiquitin-conjugating enzymes (also known as E2s) and ubiquitin ligases (also known as E3s) (Hershko and Ciechanover 1998).
The impact of ubiquitination depends on whether a single ubiquitin moiety (monoubiquitination) or an ubiquitin chain (polyubiquitination) has been attached to a protein. Monoubiquitination tends to trigger cellular processes related to endocytosis and membrane trafficking (Haglund et al. 2003) while the impact of polyubiquitination varies depending on how the ubiquitin residues in the chain have been linked. Attachment of Lysine-48 ubiquitin chains results in degradation by the 20S proteasome while addition of Lysine-63 ubiquitin chains mediates DNA damage and NFkappaB signaling (Chen 2005 and Mocciaro and Rape 2012). Lysine-6, Lysine-11, Lysine-27, Lysine-29 and Lysine-33 chains have also been reported (Komander 2009 and Ye and Rape 2011).
When comparing staining of mouse anti polyubiquitin antibody (clone FK1) against staining with mouse anti mono or polyubiquitin antibody (clone FK2) or pan ubiquitin antibody (clone P4D1) one can determine, if a protein target is mono- or polyubiquitinated.
- Target Species
- Product Form
- Purified IgM - liquid
- Purified IgM prepared by ion exchange chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Poly-ubiquitinylated lysozyme
- Approx. Protein Concentrations
- Ig concentration 1.0 mg/ml
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted.
- Shelf Life
- 12 months from date of despatch
- For research purposes only
Applications of Polyubiquitin antibody
|Application Name||Verified||Min Dilution||Max Dilution|
- Western Blotting
- Mouse anti polyubiquitin is specific for ubiquitin-protein conjugates and shows no reactivity with free ubiquitin. Use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
Secondary Antibodies Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Human anti Mouse IgM:FITC||HCA040F||0.1 mg||F|
|Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed)||STAR138A||1 ml||C E P WB|
|Goat anti Mouse IgM:RPE||STAR86PE||0.5 ml||F|
|Goat anti Mouse IgG/A/M:Alk. Phos.||STAR87A||1 mg||C E WB|
|Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)||STAR87P||1 mg||E|
Product Specific References
References for Polyubiquitin antibody
Fujimuro, M. et al (1994) Production and characterization of monoclonal antibodies specific to multi-ubiquitin chains of polyubiquitinated proteins.
FEBS Lett. 349 (2):173-80
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