Ricin antibody | RA999
The ricin toxin consists of two chains, A and B, covalently linked by a disulfide bond. The A chain acts as a glycosidase that removes an adenine residue from the 28S rRNA. The area from which the adenine is removed is essential for binding of elongation factors, destroying it leaves the ribosome inactive and unable to support protein synthesis. The A chain can inactivate a few thousand ribosomes per minute, much faster than the cell can replace them, meaning that a single ricin A chain can kill a cell.
The B chain binds to complex carbohydrates on the cell surface to facilitate entry of the A chain into the cell. Without the B chain, the A chain has very low toxicity.Ricin is easily extracted from the castor bean (Ricinus communis), and the lethal dose for humans is very small. It is toxic when inhaled, ingested or injected and there is no known antidote, although a vaccine has been developed. Treatment is symptomatic and supportive, with long term organ damage likely in survivors. The ease of extraction and distribution makes it a potential candidate for biological warfare although it is less stable than other agents, such as anthrax.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3)
- Full length Ricin A (RCA60) from Ricinus communis.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- P02879 Related reagents
- Entrez Gene
- RCOM_2159910 Related reagents
- GO Terms
- GO:0000166 nucleotide binding
- GO:0006952 defense response
- GO:0005529 sugar binding
- GO:0009405 pathogenesis
- GO:0017148 negative regulation of translation
- GO:0030598 rRNA N-glycosylase activity
- For research purposes only
Applications of Ricin antibody
|Application Name||Verified||Min Dilution||Max Dilution|
Secondary Antibodies Available
Product Specific References
References for Ricin antibody
Sokołowska, I. et al. (2011) A single point mutation in ricin A-chain increases toxin degradation and inhibits EDEM1-dependent ER retrotranslocation.
Biochem J. 436 (2): 371-85.
Sokołowska, I. et al. (2015) Hydrophobicity of protein determinants influences the recognition of substrates by EDEM1 and EDEM2 in human cells.
BMC Cell Biol. 16: 1.