TNF Alpha antibody | 6E10

Mouse anti Pig TNF Alpha:Preservative Free
- Product Type
- Monoclonal Antibody
- Clone
- 6E10
- Isotype
- IgG1
- Specificity
- TNF Alpha
Mouse anti Porcine TNF alpha antibody, clone 6E10 recognizes porcine tumour necrosis factor alpha (TNF alpha). Mouse anti Porcine TNF alpha antibody, clone 6E10 recognizes both native and recombinant TNF alpha. TNF alpha is expressed by a range of cells including macrophages and monocytes, and is an important mediator of the porcine inflammatory response. Mouse anti Porcine TNF alpha antibody, clone 6E10 is reported to neutralize the effects of porcine TNF alpha in in vitro cytotoxicity assays using WEHI 164 clone 13 murine fibrosarcoma cells (Su et al. 1992). |
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant porcine TNF alpha.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
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ELISA | ![]() |
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Flow Cytometry | ![]() |
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Functional Assays | ![]() |
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Western Blotting | ![]() |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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List Price | Your Price | ||||||
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Description | Mouse IgG1 Negative Control |
References for TNF Alpha antibody
-
Su, X. et al. (1992) Monoclonal antibodies to porcine tumor necrosis factor alpha development of an enzyme-linked immunoabsorbant assay.
J. Immunoassay 13:253-272. -
Zelnickova, P. et al. (2007) Intracellular cytokine detection by flow cytometry in pigs: fixation, permeabilization and cell surface staining.
J Immunol Methods. 327: 18-29. -
Zelnickova, P. et al. (2008) Age-dependent changes of proinflammatory cytokine production by porcine peripheral blood phagocytes.
Vet Immunol Immunopathol. 124: 367-78.
- RRID
- AB_2204233
- UniProt
- P23563
- Entrez Gene
- TNF
- GO Terms
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
MCA2360Z

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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