TNF Alpha antibody | 6E10
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Mouse anti Porcine TNF alpha antibody, clone 6E10 recognizes porcine tumour necrosis factor alpha (TNF alpha). Mouse anti Porcine TNF alpha antibody, clone 6E10 recognizes both native and recombinant TNF alpha.
TNF alpha is expressed by a range of cells including macrophages and monocytes, and is an important mediator of the porcine inflammatory response.
Mouse anti Porcine TNF alpha antibody, clone 6E10 is reported to neutralise the effects of porcine TNF alpha in in vitro cytotoxicity assays using WEHI 164 clone 13 murine fibrosarcoma cells (Su et al. 1992).
TNF alpha is expressed by a range of cells including macrophages and monocytes, and is an important mediator of the porcine inflammatory response.
Mouse anti Porcine TNF alpha antibody, clone 6E10 is reported to neutralise the effects of porcine TNF alpha in in vitro cytotoxicity assays using WEHI 164 clone 13 murine fibrosarcoma cells (Su et al. 1992).
Product Details
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant porcine TNF alpha.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
More Information
- UniProt
- P23563 Related reagents
- Entrez Gene
- TNF Related reagents
- GO Terms
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- Regulatory
- For research purposes only
Applications of TNF Alpha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Flow Cytometry | |||
| Functional Assays | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system to a concentration equivalent to their test reagent.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F |
Product Specific References
References for TNF Alpha antibody
-
Su, X. et al. (1992) Monoclonal antibodies to porcine tumor necrosis factor alpha development of an enzyme-linked immunoabsorbant assay.
J. Immunoassay 13:253-272. -
Zelnickova, P. et al. (2007) Intracellular cytokine detection by flow cytometry in pigs: fixation, permeabilization and cell surface staining.
J Immunol Methods. 327: 18-29. -
Zelnickova, P. et al. (2008) Age-dependent changes of proinflammatory cytokine production by porcine peripheral blood phagocytes.
Vet Immunol Immunopathol. 124: 367-78.
