Neurofilament L antibody | DA2

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Mouse anti Pig Neurofilament L

Product Type
Monoclonal Antibody
Clone
DA2
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA4751
Datasheet
IF WB
MSDS
0.1 ml loader

Mouse anti Pig Neurofilament L antibody, clone DA2 recognizes Neurofilament light polypeptide, also known as neurofilament L, NF-L, 68 kDa neurofilament protein or Neurofilament triplet L protein. NF-L is a 548 amino acid ~68 kDa neurofilament. NF-L is the most abundant of the three nurofilament proteins (NF-H, NF-M and NF-L). Neurofilaments are 10nm or intermediate filaments found in neurons, with especially high concentrations along the axons, where they appear to control axonal diameter.

In humans defects in the NEFL gene are the cause of Charcot-Marie-Tooth disease 1F (CMT1F), a demyelinating condition of the peripheral nervous system characterized by progressive weakness and atrophy (Jordanova et al. 2003) and the related condition Charcot-Marie-Tooth disease 2E (CMT2E), characterized by loss of muscle tissue and touch sensation with axonal degeneration in the absence of myelin alteration (De Jonghe et al. 2001).

Product Details

Target Species
Pig
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Mouse
Rat
Bovine
Chicken
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.065% Sodium Azide (NaN3)
Immunogen
Preparation of pig intermediate filaments.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P02547 Related reagents
P07196 Related reagents
P08551 Related reagents
P19527 Related reagents
P02548 Related reagents
Entrez Gene
NEFL Related reagents
NEFL Related reagents
Nefl Related reagents
Nefl Related reagents
GO Terms
GO:0005200 structural constituent of cytoskeleton
GO:0005883 neurofilament
GO:0008022 protein C-terminus binding
GO:0008089 anterograde axon cargo transport
GO:0008090 retrograde axon cargo transport
GO:0042802 identical protein binding
GO:0019896 axon transport of mitochondrion
GO:0033693 neurofilament bundle assembly
GO:0000226 microtubule cytoskeleton organization
GO:0014012 peripheral nervous system axon regeneration
GO:0031133 regulation of axon diameter
GO:0040011 locomotion
GO:0043524 negative regulation of neuron apoptosis
GO:0050772 positive regulation of axonogenesis
GO:0050885 neuromuscular process controlling balance
GO:0005829 cytosol
GO:0007268 synaptic transmission
GO:0005198 structural molecule activity
GO:0045110 intermediate filament bundle assembly
GO:0060052 neurofilament cytoskeleton organization
GO:0009636 response to toxin
GO:0010243 response to organic nitrogen
GO:0019904 protein domain specific binding
GO:0043274 phospholipase binding
GO:0043434 response to peptide hormone stimulus
GO:0051258 protein polymerization
GO:0051412 response to corticosterone stimulus
Regulatory
For research purposes only

Applications of Neurofilament L antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/100
Western Blotting 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA4751 detects a band of approximately 68kDa in rat cortex lysate.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for Neurofilament L antibody

  1. Peichl, L. & González-soriano, J. (1993) Unexpected presence of neurofilaments in axon-bearing horizontal cells of the mammalian retina.
    J Neurosci. 13 (9): 4091-100.
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