IFN Gamma antibody | AbD06477

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Human anti Pig Interferon Gamma

Product Type
Monoclonal Antibody
Clone
AbD06477
Isotype
HuCAL Fab bivalent
Product Code Applications Pack Size List Price Quantity
HCA090
Datasheet
E
MSDS
0.1 mg loader

Human anti Pig interferon gamma antibody, clone AbD06477 recognizes porcine interferon gamma (IFN gamma). IFN gamma is a type II interferon, which is structurally unrelated to type I interferons. Interferons regulate innate and adaptive immunity to viral infection. IFN gamma has anti viral activity and is secreted by a wide range of cells including T-cells, B-cells, NK cells and professional antigen presenting cells such as macrophages and dendritic cells.

HCA090 is recommended for use as a capture antibody in ELISA assays to detect native porcine IFN gamma. MCA1783B is recommended as a detection reagent.

Product Details

Target Species
Pig
Product Form
A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Recombinant porcine interferon gamma.
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml

Storage Information

Storage
Store at +4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P17803 Related reagents
Entrez Gene
IFNG Related reagents
GO Terms
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005133 interferon-gamma receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of IFN Gamma antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Histidine Tag:Alk.Phos. MCA1396A E WB 0.1 mg
Mouse anti Histidine Tag:Biotin MCA1396B E WB 0.1 mg loader
Mouse anti c-Myc:Alk.Phos. MCA2200A C E P WB* 0.1 mg
Mouse anti c-Myc:Biotin MCA2200B C E P WB* 0.1 mg loader
Mouse anti c-Myc:HRP MCA2200P C E P WB* 0.1 mg loader
Mouse anti Penta Histidine Tag:HRP MCA5995P E WB 125 µl loader