CD45RC antibody | MIL5

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Mouse anti Pig CD45RC

Product Type
Monoclonal Antibody
Clone
MIL5
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1857GA
Datasheet
F
MSDS
0.1 mg loader

Mouse anti Pig CD45RC, clone MIL5 recognizes a restricted CD45 epitope encoded by the CD45C exon. Binding to the 210 kDa RC isoform of porcine CD45, clone MIL5 recognizes an alternative epitope to that bound by clone MIL15, also against porcine CD45RC. These two clones also differ in their ability to stain B cells in tissue sections (Zuckerman, F. A. et al. 2001). Increased expression of CD45RC was observed during the maturation of porcine gamma delta thymocytes (Sinkora, et al. 2005).

Product Details

Target Species
Pig
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Porcine PBMCs
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3X.63.Ag8 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of CD45RC antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/100
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Application Based External Images

Flow Cytometry

Product Specific References

References for CD45RC antibody

  1. Zuckermann, F.A. et al. (1998) Definition of the specificity of monoclonal antibodies against porcine CD45 and CD45R: report from the CD45/CD45R and CD44 subgroup of the Second International Swine CD Workshop.
    Vet Immunol Immunopathol. 60 (3-4): 367-87.
  2. Schnitzlein, W.M. & Zuckermann, F.A. (1998) Determination of the specificity of CD45 and CD45R monoclonal antibodies through the use of transfected hamster cells producing individual porcine CD45 isoforms.
    Vet Immunol Immunopathol. 60 (3-4): 389-401.
  3. Haverson, K. et al. (1999) T-cell populations in the pig intestinal lamina propria: memory cells with unusual phenotypic characteristics.
    Immunology 96: 66-73.
  4. Zuckermann, F.A. et al. (2001) Characterization of monoclonal antibodies assigned to the CD45 subgroup of the Third International Swine CD Workshop.
    Vet Immunol Immunopathol. 80 (1-2): 165-74.
  5. Terzic, S. et al. (2002) Immunophenotyping of leukocyte subsets in peripheral blood and palatine tonsils of prefattening pigs.
    Vet Res Commun. 26: 273-83.
  6. Bozic, F. et al. (2002) Recruitment of intestinal CD45RA+ and CD45RC+ cells induced by a candidate oral vaccine against porcine post-weaning colibacillosis.
    Vet Immunol Immunopathol. 86: 137-46.
  7. Žubčić, D. (2014) Identification, localization and quantification of porcine intestinal immune cell subsets during the first seven weeks of postnatal ontogeny
    Veterinarski Archiv 84(3): 265-78.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.