Mouse anti Pig CD45RC, clone MIL5
recognizes a restricted CD45 epitope encoded by the CD45C exon. Binding to the 210 kDa RC isoform of porcine CD45, clone MIL5 recognizes an alternative epitope to that bound by clone MIL15
, also against porcine CD45RC. These two clones also differ in their ability to stain B cells in tissue sections (Zuckerman, F. A. et al. 2001
). Increased expression of CD45RC was observed during the maturation of porcine gamma delta thymocytes (Sinkora, et al. 2005
- Target Species
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Porcine PBMCs
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3X.63.Ag8 myeloma cell line
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD45RC antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
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Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Product Specific References
References for CD45RC antibody
Zuckermann, F.A. et al. (1998) Definition of the specificity of monoclonal antibodies against porcine CD45 and CD45R: report from the CD45/CD45R and CD44 subgroup of the Second International Swine CD Workshop.
Vet Immunol Immunopathol. 60 (3-4): 367-87.
Schnitzlein, W.M. & Zuckermann, F.A. (1998) Determination of the specificity of CD45 and CD45R monoclonal antibodies through the use of transfected hamster cells producing individual porcine CD45 isoforms.
Vet Immunol Immunopathol. 60 (3-4): 389-401.
Haverson, K. et al. (1999) T-cell populations in the pig intestinal lamina propria: memory cells with unusual phenotypic characteristics.
Immunology 96: 66-73.
Zuckermann, F.A. et al. (2001) Characterization of monoclonal antibodies assigned to the CD45 subgroup of the Third International Swine CD Workshop.
Vet Immunol Immunopathol. 80 (1-2): 165-74.
Terzic, S. et al. (2002) Immunophenotyping of leukocyte subsets in peripheral blood and palatine tonsils of prefattening pigs.
Vet Res Commun. 26: 273-83.
Bozic, F. et al. (2002) Recruitment of intestinal CD45RA+ and CD45RC+ cells induced by a candidate oral vaccine against porcine post-weaning colibacillosis.
Vet Immunol Immunopathol. 86: 137-46.
Žubčić, D. (2014) Identification, localization and quantification of porcine intestinal immune cell subsets during the first seven weeks of postnatal ontogeny
Veterinarski Archiv 84(3): 265-78.
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
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