CD45RA antibody | MIL13

Mouse anti Pig CD45RA:RPE

Product Type
Monoclonal Antibody
Clone
MIL13
Isotype
IgG1
Specificity
CD45RA

Product Code Applications Pack Size List Price Your Price Qty
MCA1751PE
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Mouse anti Pig CD45RA, clone MIL13, recognizes an epitope contained in the portion of porcine CD45 encoded by exon A, CD45RA (Lunney et al. 2007).

Mouse anti pig CD45RA, clone MIL13 recognizes both the 210 kDa RA CD45 isoform and the 226 kDa RAC isoform (Zuckermann et al. 2001). Clone MIL13 does not recognize the CD45RC or CD45RO isoforms.

Target Species
Pig
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Immunogen
Cells isolated from porcine mesenteric lymph node
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader
List Price Your Price
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Description Mouse IgG1 Negative Control:RPE

References for CD45RA antibody

  1. Bozić F et al. (2002) Recruitment of intestinal CD45RA+ and CD45RC+ cells induced by a candidate oral vaccine against porcine post-weaning colibacillosis.
    Vet Immunol Immunopathol. 86 (3-4): 137-46.
  2. Terzic, S. et al. (2002) Immunophenotyping of leukocyte subsets in peripheral blood and palatine tonsils of prefattening pigs.
    Vet Res Commun. 26: 273-83.
  3. Pakkanen, T.M. et al. (2000) Periadventitial lacZ gene transfer to pig carotid arteries using a biodegradable collagen collar or a wrap of collagen sheet with adenoviruses and plasmid-liposome complexes.
    J Gene Med. 2: 52-60.
  4. Schierack, P. et al. (2009) Effects of Bacillus cereus var. toyoi on immune parameters of pregnant sows.
    Vet Immunol Immunopathol. 127: 26-37.
  5. Suzuki, S. et al. (2016) Generation and characterization of RAG2 knockout pigs as animal model for severe combined immunodeficiency.
    Vet Immunol Immunopathol. 178: 37-49.
  6. López, E. et al. (2019) Identification of very early inflammatory markers in a porcine myocardial infarction model.
    BMC Vet Res. 15 (1): 91.
  7. Li, K.et al. (2019) Generation of porcine monoclonal antibodies based on single cell technologies.
    Vet Immunol Immunopathol. 215: 109913.
  8. Forner, R. et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.
    PLoS One. 16 (5): e0249366.
  9. View The Latest Product References
  10. Ogihara, K. et al. (2022) A porcine lymphoma-derived cell line co-expressing IgM, IgG and IgA.
    J Vet Med Sci. Apr 11 [Epub ahead of print].

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

RRID
AB_323626
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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