CD117 antibody | 2B8/BM

Mouse anti Pig CD117:Alexa Fluor® 488

Product Type
Monoclonal Antibody
Clone
2B8/BM
Isotype
IgG1
Specificity
CD117

Product Code Applications Pack Size List Price Your Price Qty
MCA2598A488
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Pig CD117, clone 2B8/BM is specific for porcine CD117, also known as c-kit, a 155 kDa type I transmembrane protein with protein tyrosine kinase activity, which plays an important role in early hematopoiesis (Perez et al. 2007).

Hematopoietic stem cells (HSC) of bone marrow have multilineage differentiation potential and an extensive capacity for self-renewal. The majority of adult bone marrow hematopoietic progenitor cells are CD117+ and have been used successfully in xenograft transplantation models for long term survival of grafts, without symptoms of graft-versus-host disease (GVHD).

Mouse anti pig CD117, clone 2B8/BM recognizes CD117 on a small subset of porcine bone marrow progenitor cells and therefore provides an alternative tool from the previously used c-kit ligand stem cell factor, for the isolation and enrichment of porcine stem cells.

Target Species
Pig
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Immunogen
Porcine bone marrow cells (BMC).
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mouse were fused with cells of the Sp2/0 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®488 495 519
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 488 MCA928A488 F 100 Tests/1ml loader
List Price Your Price
loader
Description Mouse IgG1 Negative Control:Alexa Fluor® 488

References for CD117 antibody

  1. Wehman, B. et al. (2017) Cardiac Progenitor Cells Enhance Neonatal Right Ventricular Function After Pulmonary Artery Banding.
    Ann Thorac Surg. 104 (6): 2045-53.
  2. Pérez C et al. (2007) Characterisation of porcine bone marrow progenitor cells identified by the anti-c-kit (CD117) monoclonal antibody 2B8/BM.
    J Immunol Methods. 321 (1-2): 70-9.
  3. Hatzistergos, K.E. et al. (2010) Bone marrow mesenchymal stem cells stimulate cardiac stem cell proliferation and differentiation.
    Circ Res. 107: 913-22.
  4. Escalona, Z. et al. (2014) Molecular characterization and expression of porcine Siglec-5.
    Dev Comp Immunol. 44 (1): 206-16.
  5. Wehman, B. et al. (2016) Mesenchymal Stem Cells Preserve Neonatal Right Ventricular Function In A Porcine Model Of Pressure Overload.
    Am J Physiol Heart Circ Physiol. Apr 22:ajpheart.00955.2015. [Epub ahead of print]
  6. Wehman, B. et al. (2016) Intracoronary Stem Cell Delivery to the Right Ventricle: A Preclinical Study.
    Semin Thorac Cardiovasc Surg. 28 (4): 817-24.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Synonyms
C-KIT
RRID
AB_1125233
UniProt
Q2HWD6
Entrez Gene
KIT
GO Terms
GO:0016021 integral to membrane
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005524 ATP binding
View more products with CD117 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Pig Products